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Hu L.,Eastern Hepatobiliary Hospital affiliated to the Second Military Medicine College | Xue F.,Eastern Hepatobiliary Hospital affiliated to the Second Military Medicine College | Shao M.,Shanghai JiaoTong University | Deng A.,Shanghai University | Wei G.,Eastern Hepatobiliary Hospital affiliated to the Second Military Medicine College
BioScience Trends | Year: 2013

Using a meta-analysis on gene expression data of hepatocellular carcinomas (HCC) from the Oncomine database, we identified that the Notch3 gene ranked as the highest up-regulated Notch pathway member in HCC tissues compared with normal liver tissues. We further detected the expression of Notch3 in 95 cases of HCC samples by immunohistochemistry, and evaluated its clinicopathological and prognostic significance. We confirmed that Notch3 is overexpressed in HCC tissues compared with normal liver tissues, and a high expression level of Notch3 was significantly associated with bigger tumor size (p = 0.014), multiple tumors (p = 0.026), late tumor-node-metastasis (TNM) stage (p < 0.01) and shorter over all survival (p = 0.002). Furthermore, high Notch3 expression emerged as a significant independent prognostic factor in multivariate analysis. In conclusion, we identified a positive association between Notch3 expression with more aggressive traits and shorter survival in HCC. Our findings suggest that Notch3 might be an important regulator in the development of HCC, and a potential therapeutic target for patients with HCC. Source


Hu L.,Eastern Hepatobiliary Hospital affiliated to the Second Military Medicine College | Xue F.,Eastern Hepatobiliary Hospital affiliated to the Second Military Medicine College | Li Y.,Eastern Hepatobiliary Hospital affiliated to the Second Military Medicine College | Shao M.,Shanghai JiaoTong University | And 2 more authors.
Cell Biochemistry and Biophysics | Year: 2014

Although HBV, liver function and tumor characteristics were proven as hepatocellular carcinoma (HCC) prognosis-related, no large-scale and long-term follow-up studies have ever given robust evidence about prognosis predictive effect and contribution to different stage of postoperation. In this study, we evaluated the influence of above index on overall survival (OS) and disease-free survival (DFS) and other clinical data in a rather large population and long-term follow-up. Our study consisted of 1,326 HCC patients who underwent radical resection from 1996 to 2010. Epidemiology, clinical and prognosis data were analyzed. Risk factors of OS and DFS were explored. Cumulative survival comparison between groups was performed with log-rank. Multivariate analysis for independent prognostic factors was determined by Cox proportional hazards model. HBsAg status was a universal factor of HCC recurrence, while preoperational albumin (ALB) and portal vein tumor thrombus (PVTT) affected survival during the whole lifetime. Early stage recurrence was associated with capsule intact [OR (95 %) = 1.54,1.12-2.12, p = 0.009], preoperational alpha-fetoprotein (AFP), TNM and BCLC stages were the most important prognosis factors of recurrence in the early 5 years and PVTT affected the rest time. Survival was mainly associated with tumor characteristic and ALB. Short-time survival was affected with age and AFP, while BCLC was related with the long-time survival. We confirmed that during different periods after resection, factors affecting prognosis did not remain unchanged. Liver function and tumor characteristic affected DFS and OS the whole time, especially the early recurrence. However, HBV infection situation was associated with later recurrence. PVTT showed an opposite effect between early and later recurrence. © 2014 Springer Science+Business Media New York. Source

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