Earth System science Organization National Institute of Ocean Technology

Chennai, India

Earth System science Organization National Institute of Ocean Technology

Chennai, India
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Keppayan T.,Anna University | Keppayan T.,Earth System science Organization National Institute of Ocean Technology | Ponnusamy L.,Anna University | Ramalingam K.,Earth System science Organization National Institute of Ocean Technology
Journal of Computational and Theoretical Nanoscience | Year: 2017

This paper describes the real-time development, controller modelling and implementation of the deep-sea environmental conditions mimicking lab system. Deep-sea environmental variables of pressure and temperature are mimicked in the fully automated PC controlled high-pressure system. The conventional PI, Genetic Algorithm (GA) and Fuzzy Logic Control (FLC) methods are used to achieve a better control action with respect to load disturbances and set point changes at elevated pressure. These parameter controllers are implemented in simulation and real-time experiment. The simulation is carried out in the MATLAB environment in which the results shows that FLC is effective in tracking set point and has resulted in a minimum value of the performance indices, peak overshoot and settling time as compared to conventional methods. The controllers are tested with servo and regulatory operations and compared in terms of time domain specification and performance index criterion. In the reactor temperature control loop the maximum overshoot of decentralized control method is 15.3% and it is significantly reduced to zero in FLC decoupled method. The experimental results show that the model accuracy and it match favorably with the simulation results. Copyright © 2017 American Scientific Publishers All rights reserved.


Inbakandan D.,Sathyabama University | Kumar C.,Sathyabama University | Bavanilatha M.,Sathyabama University | Ravindra D.N.,Sathyabama University | And 2 more authors.
Microbial Pathogenesis | Year: 2016

The knowhow followed for synthesis, characterization and application of nanomaterials has become an important branch of nanoscience. The use of marine sponges for the synthesis of metal nanoparticles is still in the budding level of current nanobiotechnology. This paper reports a single step one pot biosynthesis utilizing marine sponge (Haliclona exigua) extract as a reducing agent by means of a conventional ultrasonic bath on the formation and growth of flower like silver nanocolloids. These silver nanocolloids were characterized through UV visible spectroscopy, High Resolution Transmission Electron Microscope, Fourier Transform Infrared spectroscopy and X-ray Diffractometer. Further, antibacterial activity and antiproliferative activity were done against oral biofilm bacteria and oral cancer cell lines for the biosynthesized flower like silver nanocolloids. Water soluble organic amines were responsible for the syntheses of nanomaterials which have a size range from 100 to 120 nm. An average size of 9.1 mm zone of inhibition was recorded with 10.0 μg of silver nanocolloids against oral biofilm bacteria. The estimated half maximal inhibitory concentration value for flower like silver nanocolloids was 0.6 μg/ml for oral cancer cell lines. © 2016 Elsevier Ltd


Meshram P.,Bhabha Atomic Research Center | Dave R.,Bhabha Atomic Research Center | Joshi H.,Bhabha Atomic Research Center | Dharani G.,Earth System science Organization National Institute of Ocean Technology | And 2 more authors.
Chemosphere | Year: 2016

Polysaccharide fouling poses a significant challenge in the widespread application of membrane filtration for water purification. In order to mitigate the problem, a polysaccharide-degrading enzyme alginate lyase (Alg L; EC 4.2.2.3) was successfully immobilized on cellulose acetate ultrafiltration membrane using a dead-end filtration unit. Attenuated total reflectance Fourier transform infrared microscopy confirmed covalent linkage of the Alg L to the membrane. HPLC and Alg L activity studies confirmed that Alg L in immobilized form was enzymatically active. Even after 21 d, Alg L in immobilized form retained 80% of its original activity, compared to its free counterpart, which retained only 20% of its original activity. In fouling experiments using tap water containing 50 mg L−1 alginate, a simple backwash could remove the fouling on Alg L immobilized membrane, but not that on the control membrane. Atomic force microscopic analysis and bright field microscopic images of the fouled test membrane after backwash showed significant removal of fouling, while fouling on the control membrane remained largely intact. The immobilized Alg L remained active even after 10 runs of fouling-backwash cycle. The present antifouling technology using immobilized enzyme is suitable for keeping ultrafiltration membranes clean without the use of toxic chemical biocides. © 2016 Elsevier Ltd


Dheenan P.S.,Earth System science Organization National Institute of Ocean Technology | Jha D.K.,Earth System science Organization National Institute of Ocean Technology | Vinithkumar N.V.,Earth System science Organization National Institute of Ocean Technology | Ponmalar A.A.,Earth System science Organization National Institute of Ocean Technology | And 2 more authors.
Journal of Sea Research | Year: 2014

The purpose of this study was to determine the concentration, distribution of bacteria and physicochemical property of surface seawater in Rangat Bay, Middle Andaman, Andaman Islands (India). The bay experiences tidal variations. Perhaps physicochemical properties of seawater in Rangat Bay were found not to vary significantly. The concentration of faecal streptococci was high (2.2×103CFU/100mL) at creek and harbour area, whereas total coliforms were high (7.0×102CFU/100mL) at mangrove area. Similarly, total heterotrophic bacterial concentration was high (5.92×104CFU/100mL) in mangrove and harbour area. The Vibrio cholerae and Vibrio parahaemolyticus concentration was high (4.2×104CFU/100mL and 9×103CFU/100mL) at open sea. Cluster analysis showed grouping of stations in different tidal periods. The spatial maps clearly depicted the bacterial concentration pattern in the bay. The combined approach of multivariate analysis and spatial mapping techniques was proved to be useful in the current study. © 2013 Elsevier B.V.


PubMed | Sathyabama University, Earth System science Organization National Institute of Ocean Technology and Annamalai University
Type: | Journal: Microbial pathogenesis | Year: 2016

The knowhow followed for synthesis, characterization and application of nanomaterials has become an important branch of nanoscience. The use of marine sponges for the synthesis of metal nanoparticles is still in the budding level of current nanobiotechnology. This paper reports a single step one pot biosynthesis utilizing marine sponge (Haliclona exigua) extract as a reducing agent by means of a conventional ultrasonic bath on the formation and growth of flower like silver nanocolloids. These silver nanocolloids were characterized through UV visible spectroscopy, High Resolution Transmission Electron Microscope, Fourier Transform Infrared spectroscopy and X-ray Diffractometer. Further, antibacterial activity and antiproliferative activity were done against oral biofilm bacteria and oral cancer cell lines for the biosynthesized flower like silver nanocolloids. Water soluble organic amines were responsible for the syntheses of nanomaterials which have a size range from 100 to 120nm. An average size of 9.1mm zone of inhibition was recorded with 10.0g of silver nanocolloids against oral biofilm bacteria. The estimated half maximal inhibitory concentration value for flower like silver nanocolloids was 0.6g/ml for oral cancer cell lines.


PubMed | Earth System science Organization National Institute of Ocean Technology and Bhabha Atomic Research Center
Type: | Journal: Chemosphere | Year: 2016

Polysaccharide fouling poses a significant challenge in the widespread application of membrane filtration for water purification. In order to mitigate the problem, a polysaccharide-degrading enzyme alginate lyase (Alg L; EC 4.2.2.3) was successfully immobilized on cellulose acetate ultrafiltration membrane using a dead-end filtration unit. Attenuated total reflectance Fourier transform infrared microscopy confirmed covalent linkage of the Alg L to the membrane. HPLC and Alg L activity studies confirmed that Alg L in immobilized form was enzymatically active. Even after 21d, Alg L in immobilized form retained 80% of its original activity, compared to its free counterpart, which retained only 20% of its original activity. In fouling experiments using tap water containing 50mgL

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