Kurbasic E.,House |
Sjostrom M.,Lund University |
Krogh M.,Amber Biosciences AB |
Folkesson E.,Lund University |
And 9 more authors.
Clinical Proteomics | Year: 2015
Background: Breast cancer is a very heterogeneous disease and some patients are cured by the surgical removal of the primary tumour whilst other patients suffer from metastasis and spreading of the disease, despite adjuvant therapy. A number of prognostic and treatment predictive factors have been identified such as tumour size, oestrogen (ER) and progesterone (PgR) receptor status, human epidermal growth factor receptor type 2 (HER2) status, histological grade, Ki67 and age. Lymph node involvement is also assessed during surgery to determine if the tumour has spread which requires dissection of the axilla and adjuvant treatment. The prognostic and treatment predictive factors assessing the nature of the tumour are all routinely based on the status of the primary tumour. Results: We have analysed a unique tumour set of fourteen primary breast cancer tumours with matched synchronous axillary lymph node metastases and a set of nine primary tumours with, later developed, matched distant metastases from different sites in the body. We used a pairwise tumour analysis (from the same individual) since the difference between the same tumour-type in different patients was greater. Glycopeptide capture was used in this study to selectively isolate and quantify N-linked glycopeptides from tumours mixtures and the captured glycopeptides were subjected to label-free quantitative tandem mass spectrometry analysis. Differentially expressed proteins between primary tumours and matched lymph node metastasis and distant metastasis were identified. Two of the top hits, ATPIF1 and tubulin β-chain were validated by immunohistochemistry to be differentially regulated. Conclusions: We show that the expression of a large number of glycosylated proteins change between primary tumours and matched lymph node metastases and distant metastases, confirming that cancer cells undergo a molecular transformation during the spread to a secondary site. The proteins are part of important pathways such as cell adhesion, migration pathways and immune response giving insight into molecular changes needed for the tumour to spread. The large difference between primary tumours and lymph node and distant metastases also suggest that treatment should be based on the phenotype of the lymph node and distant metastases. © 2015 Kurbasic et al.
Raft J.,House |
Guerci P.,House |
Guerci P.,University of Lorraine |
Harter V.,Cancer Institute of Lorraine |
And 3 more authors.
Korean Journal of Anesthesiology | Year: 2015
Background: Notification of sugammadex has been supplemented with a section on hemostasis, including a longer clotting time in the first minutes following injection, without any documented clinical consequences. The objective of this observational study was to analyze the effects of sugammadex administration on routine coagulation tests and bleeding in the clinical setting. Methods: After Institutional Review Board approval, a prospective observational study was conducted between January and December 2011. Adult patients scheduled for laparotomies were analyzed in groups according to the type of reversal (without sugammadex versus 2 or 4 mg/kg sugammadex). There were no changes in our current clinical practice. Blood samples drawn from these patients were standardized at the same time and tested using the same daily calibrated machine. The endpoint was a comparison of the activated partial thromboplastin time (aPTT), prothrombin time (PT), hemoglobin (Hb) level and hematocrit (Ht), immediately before sugammadex administration (H0) and 1 h after neuromuscular block reversal (H1). Results: One hundred and forty-two patients in three groups were included as follows: 11 in the “without sugammadex” group, 64 in the “2 mg/kg sugammadex” group and 67 in the “4 mg/kg sugammadex” group. Results did not differ significantly among the groups. Conclusions: In this prospective observational study, the use of 2 and 4 mg/kg sugammadex was not associated with a longer clotting time or decreased hemoglobin concentrations. Future prospective investigations should study patients receiving 16 mg/kg sugammadex and/or with abnormal coagulation tests. © the Korean Society of Anesthesiologists, 2015.
News Article | November 3, 2016
The House of Bread Bakery Cafe Founder, Sheila McCann opened the first House of Bread 20 years ago in San Luis Obispo, California. After practicing law as a public defender, Ms. McCann decided to embark on a more positive career path. She remembered baking bread with her beloved Grandma, and all the wonderful feelings from the aromas, to the sense of accomplishment when tasting the delicious baked goods. House of Bread was designed to give the customers a replication of that “feeling” with an open production site to watch the bakers hand shape the loaves, view the finished products coming out of the oven and tasting a slice from the huge sample board. As markets change and the selection of bread choices increased in the supermarket, House of Bread expanded their product line to include a café component of breakfast and lunch…while still keeping that same “feeling” of showcasing the art of baking wholesome and nutritious bread. To celebrate the 20 year anniversary of the House of Bread Bakery and Café, there will be 20 cent cinnamon rolls for every customer as well as a chance to win one of 20, $20 gift cards. On top of that, we will be making a 20 pounds cinnamon roll. The gooey concoction is based on the same recipe that is used daily to make the popular single serve roll. “The only difference between our popular roll and this monster is that it is 50 times larger, and we give this one away to our customers through out the day.” said Sheila McCann. The House of Bread Bakery makes everything from scratch daily providing families with the most nutritious bakery products available. Customers get to watch and participate in weekly classes on bread making. The recipes have been in the family for decades, but Sheila has always felt that sharing her love of bread making and what she learned making bread with her Grandma years ago on their Montana ranch was giving something back to the community she serves. About House of Bread The House of Bread was founded in 1996, with corporate offices in San Luis Obispo, California. There are currently 8 franchise locations located within the United States. For information on the House of Bread Franchise, call our Franchise Sales Director, John Armatas at 855-844-8377.
News Article | November 1, 2016
SAN JOSE, Calif., Nov. 1, 2016 /PRNewswire/ -- House of Jane, a leading cannabis-infused beverage company in California known for its innovation and introduction of THC in caffeinated coffees and teas, expands its line to now include hemp derived CBD-infused energy supplements. Photo...