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Grant
Agency: Cordis | Branch: FP7 | Program: CP | Phase: ENV.2013.6.2-2 | Award Amount: 16.30M | Year: 2013

SOLUTIONS will deliver a conceptual framework for the evidence-based development of environmental and water policies. This will integrate innovative chemical and effect-based monitoring tools with a full set of exposure, effect and risk models and assessment options. Uniquely, SOLUTIONS taps (i) expertise of leading European scientists of major FP6/FP7 projects on chemicals in the water cycle, (ii) access to the infrastructure necessary to investigate the large basins of Danube and Rhine as well as relevant Mediterranean basins as case studies, and (iii) innovative approaches for stakeholder dialogue and support. In particular, International River Commissions, EC working groups and water works associations will be directly supported with consistent guidance for the early detection, identification, prioritization, and abatement of chemicals in the water cycle. A user-friendly tool providing access to a set of predictive models will support stakeholders to improve management decisions, benefiting from the wealth of data generated from monitoring and chemical registration. SOLUTIONS will give a specific focus on concepts and tools for the impact and risk assessment of complex mixtures of emerging pollutants, their metabolites and transformation products. Analytical and effect-based screening tools will be applied together with ecological assessment tools for the identification of toxicants and their impacts. Beyond state-of-the-art monitoring and management tools will be elaborated allowing risk identification for aquatic ecosystems and human health. The SOLUTIONS approach will provide transparent and evidence-based lists of River Basin Specific Pollutants for the case study basins and support the review of the list of WFD priority pollutants.


Dahlberg C.J.,Metagenics | Harris G.,Dynamic Extractions Inc. | Urban J.,Metagenics | Tripp M.L.,Metagenics | And 2 more authors.
Journal of Separation Science | Year: 2012

Commercially available hops (Humulus lupulus L.) bitter acid extracts contain a mixture of three major congeners (co-, n-, and ad-) in addition to cis/trans diastereomers for each congener. Individual isomerized α-acids were obtained by the consecutive application of two separate countercurrent chromatography methods. First, individual isomerized α-acid congeners as a mixture of cis/trans diastereomers were obtained using a solvent system consisting of hexane and aqueous buffer. The second purification, capable of separating cis/trans diastereomers, was accomplished using a quaternary solvent system; an alternative procedure using β-cyclodextrin followed by countercurrent chromatography was also investigated. The NaBH 4 reduction of the purified isomerized α-acid compounds followed by countercurrent chromatography purification resulted in individual ρ iso α-acids (>95%). Similarly, catalytic hydrogenation of the purified isomerized α-acid compounds followed by countercurrent chromatography purification produced individual tetrahydro isomerized α-acids (>95%). Reported herein is a widely applicable approach that focuses on three critical variables - solvent system composition, pH, and buffer-to-sample ratio - that enable the efficient purification of individual bitter acids (≥95%) from commercially available hops extracts. © 2012 Wiley-VCH Verlag GmbH & Co. KGaA.


Grant
Agency: Cordis | Branch: FP7 | Program: CP-TP | Phase: KBBE.2013.3.2-02 | Award Amount: 10.08M | Year: 2013

The D-Factory aims to set a world benchmark for a sustainable biorefinery based on biomass from halophilic microalgae. Representing the largest (100s ha) of current commercial cultivation technologies for any microalga, Dunaliella microalgal biomass production uses raceways and lakes, and will be expanded with biorefinery concepts by drawing in European innovations in key biomass processing technologies: supercritical CO2; high performance counter-current chromatography; and the use of membranes, to produce carotenes and other bioactive compounds, emulsifiers and polymers. Combining this force with world-renowned expertise in the biochemistry of Dunaliella (Ben-Amotz) we will tailor the productivities of strains sourced by the Marine Biological Association for biorefinery requirements and add to the mix, experience in constructing and using the two most advanced systems for cultivating microalgae: a series of photobioreactors developed by A4F Portugal - currently scaled-up to the largest size in the world, 1.100 m3, and open raceways by NBT Israel - 10 ha in operation for \ 30 years. Novel harvesting technology will be developed based on spiral plate technology and ultramembrane filtration. Within 36 months we will be ready to showcase a sustainable D-Factory demonstration in Europe. Designs, flowsheets and integrated schemes along with sustainability assessments (technological, environmental, economic and social) will produce benchmarks for a wide range of products and paths. These will be used in the D-Factory business case developed by Hafren Investments to raise investment for the first prototype D-Factory in Europe. The D-Factory demonstration is scheduled to be operational in 48 months. It will reach stakeholders across the globe via an Innovation Platform and will serve as a robust manifestation for the business case for global investment in algae biorefineries and in large-scale production of microalgae using photobioreactors, algal raceways and lakes.


DeAmicis C.,Dow AgroSciences | Edwards N.A.,Dynamic Extractions Ltd. | Giles M.B.,Dynamic Extractions Ltd. | Harris G.H.,Dynamic Extractions Inc. | And 3 more authors.
Journal of Chromatography A | Year: 2011

Reversed phase HPLC (RP-HPLC) and high performance countercurrent chromatography (HPCCC) were compared for the pilot scale purification of two semi-synthetic spinosyns, spinetoram-J and spinetoram-L, the major components of the commercial insecticide spinetoram. Two, independently performed, 1. kg, purification campaigns were compared. Each method resulted in the isolation of both components at a purity of >97% and yields for spinetoram-J and spinetoram-L of >93% and ≥63% of theoretical, respectively. The HPCCC process produced a 2-fold higher throughput and consumed approximately 70% less solvent than preparative scale RP-HPLC, the volume of product containing fractions from HPCCC amounted to 7% of that produced by HPLC and so required much less post-run processing. © 2011 Elsevier B.V.


Grant
Agency: GTR | Branch: Innovate UK | Program: | Phase: Collaborative Research & Development | Award Amount: 89.77K | Year: 2013

The collaboration Dynamic Extractions, Novartis UK and Imperial College supported by the TSB will overcome the last major barrier to the widespread adoption of High Performance Countercurrent Chromatography (HPCCC). This barrier is the time consuming process of developing the correct combination of solvents to separate any given chemical mixture to obtain only the desired chemical. The collaborators will create a fast and effective standard approach to select the best mixture of solvents from a standard set of solvent combinations to target the desired chemical. This will benefit the UKs pharmaceutical companies by allowing more chemicals to be purified leading to the availability of more and better drugs that will improve public health. Also as HPCCC is easily scalable (does not use expensive solid stationary phases and thus uses significantly less quantities of solvents than existing processes) the development of these new drugs will be less expensive and greener.


Roemer T.,Merck Frosst Canada Ltd. | Xu D.,Merck Frosst Canada Ltd. | Xu D.,WuXi AppTec | Singh S.B.,Merck And Co. | And 5 more authors.
Chemistry and Biology | Year: 2011

Starting with the discovery of penicillin, the pharmaceutical industry has relied extensively on natural products (NPs) as an unparalleled source of bioactive small molecules suitable for antibiotic development. However, the discovery of structurally novel and chemically tractable NPs with suitable pharmacological properties as antibiotic leads has waned in recent decades. Today, the repetitive "rediscovery" of previously known NP classes with limited antibiotic lead potential dominates most industrial efforts. This limited productivity, exacerbated by the significant financial and resource requirements of such activities, has led to a broad de-emphasis of NP research by most pharmaceutical companies, including most recently Merck. Here we review our strategies - both technological and philosophical - in addressing current antifungal discovery bottlenecks in target identification and validation and how such efforts may improve NP-based antimicrobial discoveries when aligned with NP screening and dereplication. © 2011 Elsevier Ltd. All rights reserved.


Guzlek H.,Imperial College London | Guzlek H.,Dynamic Extractions Ltd. | Baptista I.I.R.,Imperial College London | Wood P.L.,Dynamic Extractions Ltd. | Livingston A.,Imperial College London
Journal of Chromatography A | Year: 2010

Literature lists a number of counter-current chromatography (CCC) models that can predict the retention time and to a certain extent the peak width of a solute eluting from a CCC column. The approach described in this paper distinguishes itself from previous reports by relating all model parameters directly to column dimensions and experimental settings. Most importantly, this model can predict a chromatogram from scratch without resorting to traditional calibration using empirical values. The model validation with experimental results obtained across a range of CCC instruments demonstrated that the solute retention time, peak width, and peak resolution could be predicted within reasonable accuracy. Additionally, the effect of several process parameters, such as mobile phase flow rate, rotational speed of the column or β-value, showed that the model is robust and applicable to a wide range of CCC instruments. Overall, this model proved to be a useful tool for parameter estimation and, most significantly, separation optimisation. © 2010.


Wood P.L.,Dynamic Extractions Ltd.
Journal of Chromatography A | Year: 2010

I-type, J-type and non-synchronous centrifuges are all coil planet centrifuges. Analysing the motion of I-type and J-type centrifuges has advanced the understanding of how to manufacture and use these centrifuges. This paper analyses the motion of non-synchronous centrifuges producing equations of motion that can be applied to all coil planet centrifuges. This has also produced simple equations to determine the critical β-values for any coil planet centrifuge. This paper also demonstrates that I-type centrifuges also have 2 critical β-values when it was thought that β-value did not influence the understanding of the processes within I-type centrifuges. For the I-type instrument both of these critical values are at bobbin radii approaching infinity. In practice this means all I-types function within one β-value range hence the unilateral distribution and type/effectiveness of the mixing is consistent. Finally the paper shows the influence that the tangential velocity has on the Archimedean screw effect and thus the unilateral distribution of the upper and lower phases in the columns of coil planet centrifuges. This explains why the maximum stationary phase retention in an I-type centrifuge is limited to 50%. © 2009 Elsevier B.V. All rights reserved.


PubMed | Pfizer and Dynamic Extractions Inc.
Type: Journal Article | Journal: Journal of separation science | Year: 2016

A fully automated countercurrent chromatography system has been constructed to rapidly screen the commonly used heptane/ethyl acetate/methanol/water solvent system series and translate the results to preparative scale separations. The system utilizes on-demand preparation of the heptane/ethyl acetate/methanol/water solvent system upper and lower phases. Elution-extrusion countercurrent chromatography was combined with non-dynamic equilibrium injection reducing the screening time for each heptane/ethyl acetate/methanol/water system to 17 min. The result enabled solvent system development to be reduced to under 2 h. The countercurrent chromatography system was interfaced with a mass spectrometer to allow selective detection of target components in crude medicinal chemistry reaction mixtures. Mass-directed preparative countercurrent chromatography purification was demonstrated for the first time using a synthetic tetrazole epoxide derived from a routine medicinal chemistry support workflow.


Patent
DYNAMIC EXTRACTIONS Ltd | Date: 2012-09-28

The invention relates to centrifuge apparatus of a type which is typically, although not necessarily exclusively, for use in counter current chromatography in which substances are caused to partition between two phases in a column typically in the form of a helix or spiral. The apparatus includes leads which connect the inlet and outlet conduits to a column which is moved by the apparatus and in accordance with the invention the leads are constrained within a sheath which includes a lubricant to allow lubrication of the same while the apparatus is in use and thereby increase the longevity of the apparatus. The opposing ends of the leads can also be constrained in terms of movement with, at one end of the leads being fully constrained and the opposing end the leads being constrained in terms of rotational movement.

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