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Lux G.,TU Munich | Langer A.,TU Munich | Langer A.,Dynamic Biosensors GmbH | Pschenitza M.,TU Munich | And 5 more authors.
Analytical Chemistry | Year: 2015

The toxic nature of polycyclic aromatic hydrocarbons (PAHs), in particular benzo[a]pyrene (B[a]P), neccessitates the monitoring of PAH contamination levels in food and the environment. Here we introduce an indirect immunoassay format using electro-switchable biosurfaces (ESB) for the detection of B[a]P in water. The association of anti-B[a]P antibodies to microelectrodes is analyzed in real-time by measuring changes in the oscillation dynamics of DNA nanolever probes, which are driven to switch their orientations by high-frequency electrical actuation. From the association kinetics, the active concentration of anti-B[a]P, and hence the B[a]P contamination of the sample, can be determined with picomolar sensitivity. The detection limit of the assay improves with measurement time because increasingly accurate analyses of the binding kinetics become possible. It is demonstrated that an exceedance of the permissible 10 ng/L (40 pM) limit for B[a]P is detectable in an unprecedented short assay time (<1 h), using a simple three-step workflow involving minimal sample preparation. The reproducibility was satisfying with standard deviations below 5%. Further, the utility of the assay for practical applications is exemplified by analyzing a river water sample. © 2015 American Chemical Society.


Langer A.,TU Munich | Langer A.,Dynamic Biosensors GmbH | Kaiser W.,TU Munich | Kaiser W.,Dynamic Biosensors GmbH | And 4 more authors.
Journal of Physical Chemistry B | Year: 2014

Self-assembled monolayers of charged polymers are an integral component of many state-of-the-art nanobiosensors. Electrical interactions between charged surfaces and charged biomolecules, adopting the roles of linkers or capture molecules, are not only crucial to the sensor performance but may also be exploited for novel sensing concepts based on electrically actuated interfaces. Here we introduce an analytical model describing the behavior of double-stranded DNA and proteins tethered to externally biased microelectrodes. Continuum electrostatic Poisson-Boltzmann models and the drift-diffusion (Smoluchowski) equation are used to calculate the steady state as well as the dynamic behavior of oligonucleotide rods in DC and AC electric fields. The model predicts the oligonucleotide orientation on the surface and calculates how the increased hydrodynamic drag caused by a protein bound to the DNA's distal end affects the molecular dynamics of the DNA-protein complex. The results of the model are compared to experiments with electrically switchable DNA layers, and very good agreement between theory and experiment is found. The hydrodynamic diameter of the bound protein can be analyzed from experimental data of the slowed motion of the DNA-protein conjugate with angstrom precision. © 2013 American Chemical Society.


Patent
Dynamic Biosensors GmbH | Date: 2014-01-22

The present invention is directed to sequencing of nucleic acids. A method is provided for sequencing based on immobilized nucleic acid on a surface. Advantageously, a long range detection mechanism is used for detecting, whether a nucleotide provided to the substrate of a biochip has been incorporated into the immobilized template nucleic acid. Various different alignment means are provided by the present invention which can be used for facilitating a rigidly locking of the orientation of the DNA complex, which complex comprises the template nucleic acid, the primer and the capture nucleic acid. Various different linker systems may be used to immobilize the DNA complex at a first and a second strand end, such that the desired alignment of the DNA complex is achieved. Also co-adsorbed molecules on the substrate surface can be used for such an aligning measure. Additionally, or alternatively, an electrical field may be applied for repelling the DNA complex from the electrode and for facilitating a vertical DNA complex orientation. Advantageously, label-free nucleotides can be used, if desired.


Patent
Roche Holding AG and Dynamic Biosensors GmbH | Date: 2015-02-03

The present invention relates to a method of identifying a nucleotide at a defined position and determining the sequence of a target polynucleotide using an electro-switchable biosensor, as well as devices comprising an electro-switchable biosensor and uses thereof.


Patent
Dynamic Biosensors GmbH | Date: 2015-09-17

Arrangements are described for evaluating characteristics of target molecules. A biochip is received which includes a substrate to which charged probe molecules are attached. The probe molecules have a marker to allow generating signals indicative of the distance of a portion of the probe molecule from the substrate. The signals are detected and means for an external electric field is generated to which the probe molecules are exposed. A control means acts to: (A) apply an external electric field causing the portion of the probe molecule to approach the substrate, and (B) apply an external electric field causing the portion of the probe molecule to move away from the substrate. The signal is recorded as a function of time during step (A) and/or step (B). Steps (A) and (B) are repeated for a predetermined number of times and the recorded signals are combined.


Dynamic Biosensors GmbH | Entity website

TheswitchBUILD software is an easy-to-use graphical planning tool to design and program experimental workflows. Simply dragndrop individual building blocks such as kinetics, protein diameter, or melting into the measurement queue to create the workflow of your choice ...


PubMed | University of Sussex and Dynamic Biosensors GmbH
Type: | Journal: Nature communications | Year: 2017

The Structural Maintenance of Chromosomes (SMC) complexes: cohesin, condensin and Smc5/6 are involved in the organization of higher-order chromosome structure-which is essential for accurate chromosome duplication and segregation. Each complex is scaffolded by a specific SMC protein dimer (heterodimer in eukaryotes) held together via their hinge domains. Here we show that the Smc5/6-hinge, like those of cohesin and condensin, also forms a toroidal structure but with distinctive subunit interfaces absent from the other SMC complexes; an unusual molecular latch and a functional hub. Defined mutations in these interfaces cause severe phenotypic effects with sensitivity to DNA-damaging agents in fission yeast and reduced viability in human cells. We show that the Smc5/6-hinge complex binds preferentially to ssDNA and that this interaction is affected by both latch and hub mutations, suggesting a key role for these unique features in controlling DNA association by the Smc5/6 complex.


Trademark
Dynamic Biosensors GmbH | Date: 2014-02-06

Biosensors; analysers; testing apparatus not for medical purposes; optical sensors; electro-optical sensors; electronic sensors; photo-electric sensors. Apparatus for use in medical analysis; testing apparatus for medical purposes; immunological analysers; physical analysers for medical use; haematology analysers. Research relating to biotechnology; research in the field of biotechnology; scientific research in the field of medicine.


PubMed | TU Munich and Dynamic Biosensors GmbH
Type: Journal Article | Journal: Analytical chemistry | Year: 2015

The toxic nature of polycyclic aromatic hydrocarbons (PAHs), in particular benzo[a]pyrene (B[a]P), neccessitates the monitoring of PAH contamination levels in food and the environment. Here we introduce an indirect immunoassay format using electro-switchable biosurfaces (ESB) for the detection of B[a]P in water. The association of anti-B[a]P antibodies to microelectrodes is analyzed in real-time by measuring changes in the oscillation dynamics of DNA nanolever probes, which are driven to switch their orientations by high-frequency electrical actuation. From the association kinetics, the active concentration of anti-B[a]P, and hence the B[a]P contamination of the sample, can be determined with picomolar sensitivity. The detection limit of the assay improves with measurement time because increasingly accurate analyses of the binding kinetics become possible. It is demonstrated that an exceedance of the permissible 10 ng/L (40 pM) limit for B[a]P is detectable in an unprecedented short assay time (<1 h), using a simple three-step workflow involving minimal sample preparation. The reproducibility was satisfying with standard deviations below 5%. Further, the utility of the assay for practical applications is exemplified by analyzing a river water sample.


This report studies Biochip Products in Global Market, especially in North America, Europe, China, Japan, Korea and Taiwan, focuses on top manufacturers in global market, with production, price, revenue and market share for each manufacturer, covering  Illumina  Thermo Fisher Scientific  Cellix  GE Healthcare  Randox  BioDot  PerkinElmer  Roche  Horiba  Greiner Bio One International  Korea Materials & Analysis  Capitalbio  Dynamic Biosensors Market Segment by Regions, this report splits Global into several key Regions, with production, consumption, revenue, market share and growth rate of Biochip Products in these regions, from 2011 to 2021 (forecast), like  North America  Europe  China  Japan  Korea  Taiwan  Split by product type, with production, revenue, price, market share and growth rate of each type, can be divided into  Type I  Type II  Type III  Split by application, this report focuses on consumption, market share and growth rate of Biochip Products in each application, can be divided into  Application 1  Application 2  Application 3 Global Biochip Products Market Research Report 2016  1 Biochip Products Market Overview  1.1 Product Overview and Scope of Biochip Products  1.2 Biochip Products Segment by Type  1.2.1 Global Production Market Share of Biochip Products by Type in 2015  1.2.2 Type I  1.2.3 Type II  1.2.4 Type III  1.3 Biochip Products Segment by Application  1.3.1 Biochip Products Consumption Market Share by Application in 2015  1.3.2 Application 1  1.3.3 Application 2  1.3.4 Application 3  1.4 Biochip Products Market by Region  1.4.1 North America Status and Prospect (2011-2021)  1.4.2 Europe Status and Prospect (2011-2021)  1.4.3 China Status and Prospect (2011-2021)  1.4.4 Japan Status and Prospect (2011-2021)  1.4.5 Korea Status and Prospect (2011-2021)  1.4.6 Taiwan Status and Prospect (2011-2021)  1.5 Global Market Size (Value) of Biochip Products (2011-2021) 2 Global Biochip Products Market Competition by Manufacturers  2.1 Global Biochip Products Production and Share by Manufacturers (2015 and 2016)  2.2 Global Biochip Products Revenue and Share by Manufacturers (2015 and 2016)  2.3 Global Biochip Products Average Price by Manufacturers (2015 and 2016)  2.4 Manufacturers Biochip Products Manufacturing Base Distribution, Sales Area and Product Type  2.5 Biochip Products Market Competitive Situation and Trends  2.5.1 Biochip Products Market Concentration Rate  2.5.2 Biochip Products Market Share of Top 3 and Top 5 Manufacturers  2.5.3 Mergers & Acquisitions, Expansion 3 Global Biochip Products Production, Revenue (Value) by Region (2011-2016)  3.1 Global Biochip Products Production by Region (2011-2016)  3.2 Global Biochip Products Production Market Share by Region (2011-2016)  3.3 Global Biochip Products Revenue (Value) and Market Share by Region (2011-2016)  3.4 Global Biochip Products Production, Revenue, Price and Gross Margin (2011-2016)  3.5 North America Biochip Products Production, Revenue, Price and Gross Margin (2011-2016)  3.6 Europe Biochip Products Production, Revenue, Price and Gross Margin (2011-2016)  3.7 China Biochip Products Production, Revenue, Price and Gross Margin (2011-2016)  3.8 Japan Biochip Products Production, Revenue, Price and Gross Margin (2011-2016)  3.9 Korea Biochip Products Production, Revenue, Price and Gross Margin (2011-2016)  3.10 Taiwan Biochip Products Production, Revenue, Price and Gross Margin (2011-2016) For more information or any query mail at [email protected]

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