Ozbalak M.,Istanbul University |
Tokatli I.,Vkv American Hospital |
Ozdemirli M.,Georgetown University |
Tecimer T.,Acibadem University |
And 5 more authors.
European Journal of Haematology | Year: 2013
Primary effusion lymphoma (PEL) is a human herpesvirus 8 (HHV8) associated lymphoproliferative disease characterized by effusions in body cavities, and lack of tumor mass. Valganciclovir is a treatment option in PEL, however, little is known about its clinical efficacy. Ganciclovir has been reported to be effective in HHV8(+) multicentric Castleman's disease (MCD) by decreasing the plasma HHV8 load, which is an important factor in the induction and persistence of MCD, Kaposi's sarcoma (KS), and PEL. But there is no information about the efficacy of valganciclovir on HHV8 associated lymphoproliferative diseases. Here, we present the first EBV and HIV negative, HHV8 positive PEL case treated with valganciclovir; for whom it initially reduced the viral load leading to a transient partial improvement in the clinical status, but failed to induce a complete and durable remission. © 2013 John Wiley & Sons A/S.
Kaya N.,King Faisal Specialist Hospital And Research Center |
Al-Owain M.,King Faisal Specialist Hospital And Research Center |
Al-Owain M.,Alfaisal University |
Abudheim N.,King Faisal Specialist Hospital And Research Center |
And 10 more authors.
American Journal of Medical Genetics, Part A | Year: 2011
The GM2 gangliosidose, Tay-Sachs and Sandhoff diseases, are a class of lysosomal storage diseases in which relentless neurodegeneration results in devastating neurological disability and premature death. Primary prevention is the most effective intervention since no effective therapy is currently available. An extremely successful model for the prevention of GM2 gangliosidosis in the Ashkenazi Jewish community is largely attributable to the very limited number of founder mutations in that population. Consistent with our previous observation of allelic heterogeneity in consanguineous populations, we show here that these diseases are largely caused by private mutations which present a major obstacle in replicating the Ashkenazi success story. Alternative solutions are proposed which can also be implemented for other autosomal recessive diseases in our population. © 2011 Wiley-Liss, Inc.
Turksoy N.,Simurg Psychiatry and Psychotherapy center |
Bilici R.,Erenkoy state hospital for Psychiatric and Neurological Diseases |
Yalciner A.,Duzen Laboratories |
Ozdemir Y.O.,Erenkoy state hospital for Psychiatric and Neurological Diseases |
And 3 more authors.
Neuropsychiatric Disease and Treatment | Year: 2014
It is known that elevated serum homocysteine, decreased folate, and low vitamin B12 serum levels are associated with poor cognitive function, cognitive decline, and dementia. Current literature shows that some psychiatric disorders, mainly affective and psychotic ones, can be related to the levels of vitamin B12, folate, and homocysteine. These results can be explained by the importance of vitamin B12, folate, and homocysteine in carbon transfer metabolism (methylation), which is required for the production of serotonin as well as for other monoamine neurotransmitters and catecholamines. Earlier studies focused on the relationship between folate deficiency, hyperhomocysteinemia, and depressive disorders. Although depressive and anxiety disorders show a common comorbidity pattern, there are few studies addressing the effect of impaired one-carbon metabolism in anxiety disorders – especially in obsessive–compulsive disorder (OCD). This study aimed to measure the levels of vitamin B12, folate, and homocysteine specifically in order to see if eventual alterations have an etiopathogenetic significance on patients with OCD. Serum vitamin B12, folate, and homocysteine concentrations were measured in 35 patients with OCD and 22 controls. In addition, the Structured Clinical Interview for the Diagnostic and Statistical Manual for Mental Disorders, Fourth Edition, Text Revision, Yale–Brown Obsessive Compulsive Scale, Hamilton Rating Scale for Depression, and Hamilton Rating Scale for Anxiety were conducted for each patient. It was found that vitamin B12 levels were decreased and homocysteine levels were increased in some OCD patients. Homocysteine levels were positively correlated with Yale–Brown compulsion and Yale–Brown total scores. In conclusion, findings of this study suggest that some OCD patients might have vitamin B12 deficiency and higher homocysteine levels. © 2014 Türksoy et al.
Colak D.,King Faisal Specialist Hospital And Research Center |
Chishti M.A.,King Saud University |
Al-Bakheet A.,King Faisal Specialist Hospital And Research Center |
Al-Qahtani A.,King Faisal Specialist Hospital And Research Center |
And 7 more authors.
Molecular Cancer | Year: 2010
Background: Hepatocellular carcinoma (HCC) is the third-leading cause of cancer-related deaths worldwide. It is often diagnosed at an advanced stage, and hence typically has a poor prognosis. To identify distinct molecular mechanisms for early HCC we developed a rat model of liver regeneration post-hepatectomy, as well as liver cells undergoing malignant transformation and compared them to normal liver using a microarray approach. Subsequently, we performed cross-species comparative analysis coupled with copy number alterations (CNA) of independent early human HCC microarray studies to facilitate the identification of critical regulatory modules conserved across species.Results: We identified 35 signature genes conserved across species, and shared among different types of early human HCCs. Over 70% of signature genes were cancer-related, and more than 50% of the conserved genes were mapped to human genomic CNA regions. Functional annotation revealed genes already implicated in HCC, as well as novel genes which were not previously reported in liver tumors. A subset of differentially expressed genes was validated using quantitative RT-PCR. Concordance was also confirmed for a significant number of genes and pathways in five independent validation microarray datasets. Our results indicated alterations in a number of cancer related pathways, including p53, p38 MAPK, ERK/MAPK, PI3K/AKT, and TGF-β signaling pathways, and potential critical regulatory role of MYC, ERBB2, HNF4A, and SMAD3 for early HCC transformation.Conclusions: The integrative analysis of transcriptional deregulation, genomic CNA and comparative cross species analysis brings new insights into the molecular profile of early hepatoma formation. This approach may lead to robust biomarkers for the detection of early human HCC. © 2010 Colak et al; licensee BioMed Central Ltd.
Diniz G.,Tepecik Research Hospital |
Tosun Yildirim H.,Research Hospital |
Akinci G.,Research Hospital |
Hazan F.,Research Hospital |
And 4 more authors.
Pediatric Neurology | Year: 2014
Background The sarcoglycan alpha gene, also known as the adhalin gene, is located on chromosome 17q21; mutations in this gene are associated with limb-girdle muscular dystrophy type 2D. We describe two Turkish siblings with findings consistent with limb-girdle muscular dystrophy type 2D. The evaluation excluded a dystrophinopathy, which is the most common form of muscular dystrophy. Patients Both siblings had very high levels of creatinine phosphokinase and negative molecular tests for deletions and duplications of the dystrophin gene. The older boy presented at 8 years of age with an inability to climb steps and an abnormal gait. His younger brother was 5 years old and had similar symptoms. The muscle biopsy evaluation was performed only in the older brother. Results The muscle biopsy showed dystrophic features as well as a deficiency in the expression of two different glycoproteins: the alpha sarcoglycan and the gamma sarcoglycan. Sarcolemmal expressions of dystrophin and other sarcoglycans (beta and delta) were diffusely present. DNA analysis demonstrated the presence of previously unknown homozygous mutations [c.226 C > T (p.L76 F)] in exon 3 in the sarcoglycan alpha genes of both siblings. Similar heterozygous point mutations at the same locus were found in both parents, but the genes of beta, delta, and gamma sarcoglycan were normal in the remaining family members. Conclusions We describe two siblings with limb-girdle muscular dystrophy type 2D with a novel missense mutation. These patients illustrate that the differential diagnosis of muscular dystrophies is impossible with clinical findings alone. Therefore, a muscle biopsy and DNA analysis remain essential methods for diagnosis of muscle diseases. © 2014 Elsevier Inc. All rights reserved.
PubMed | Tepecik Research Hospital, Ege University, Duzen Laboratories and Dr Behcet Uz Childrens Research Hospital
Type: Journal Article | Journal: Balkan journal of medical genetics : BJMG | Year: 2016
Limb-girdle muscular dystrophy type 2E (LGMD-2E) is caused by autosomal recessive defects in the beta sarcoglycan (
Karakaya A.,Ankara University |
Bozkoyunlu G.,Ankara University |
Laleli Y.,Duzen Laboratories |
Takac S.,Ankara University
Desalination and Water Treatment | Year: 2014
Abstract: During the treatment of olive mill wastewater (OMW) by Rhodotorula glutinis, the pH of the medium increases in accordance with cell accumulation and biodegradation. Considering the pH of the medium as an indicator of biodegradation, an operation strategy depending on the pH adjustment of the batch medium with intermittent additions of OMW was developed. The addition of OMW into the biodegradation medium decreased the medium’s pH; fresh substrate was provided to the cells and the cells were kept active for biodegradation up to a critical pH value. This approach also provided the biodegradation of high volumes of undiluted OMW. About five- and twofold enhancements in total phenol removal rate were obtained in batch biodegradation but with interval feedings being carried out by free and immobilized cells, respectively, relative to the corresponding batch-wise operations. © 2013, © 2013 Balaban Desalination Publications. All rights reserved.
Karakaya A.,Ankara University |
Laleli Y.,Duzen Laboratories |
Takac S.,Ankara University
International Biodeterioration and Biodegradation | Year: 2012
Olive mill wastewater (OMW) generated during olive oil production has detrimental effects on environment arising from its high phenolics content. This study has focused on finding operational conditions of a process that biodegrade undiluted, un-pretreated and non-supplemented OMW that is raw OMW by nonconventional yeast Rhodotorula glutinis with a high dephenolization degree. The pH of OMW medium increased in accordance with cell growth and dephenolization. High initial medium pH provided by urea resulted in high phenol removal. Sterilization and centrifugation of OMW before biodegradation have no considerable effects on phenol removal. Better results were obtained at 30 °C and 150 rpm as compared to their lower values. Reduction of 89% in total phenol concentration in raw OMW was obtained in batch-wise operation where pH of the medium increased up to 8.9. The important novelty presented in this report deals with the utilization of raw OMW in biodegradation by R. glutinis. © 2012 Elsevier Ltd.
Yesil A.,Haydarpasa Numune Training and Research Hospital |
Babacan Abanonu G.,Haydarpasa Numune Training and Research Hospital |
Colak Y.,Istanbul Medeniyet University |
Paker N.,Duzen Laboratories |
Gonen C.,Haydarpasa Numune Training and Research Hospital
Gastroenterology Research and Practice | Year: 2013
Background. To investigate the relationship between DR-70 serum levels and dysplastic colon polyps. Materials and Methods. A total of 130 patients with adenomatous polyps detected by colonoscopy and divided into two groups including low versus high grade polyp, along with 50 healthy blood donors were included in the study. Blood samples from each participant were analyzed for serum CEA and DR-70 levels. Results. No statistically significant differences were observed between the two groups in terms of age or gender. The median DR-70 level was 0.5 g/mL in the healthy control group and 1.1 g/mL in group 1b (i.e., the high grade polyp) (P < 0.001). DR-70 was higher in group 1b as compared to group 1a (P < 0.001). However, the median DR-70 values for the low grade polyp group (i.e., group 1a) and the control group were similar (P = 0.067). In order to determine independent predictors of high grade dysplasia, CEA, DR-70, polyp size, and age parameters were subjected to multiple logistical regression analyses via the Enter method; the model was statistically significant (P < 0.001). Conclusions. DR-70, a marker used to measure FDP, which is generated by all major cancers, is a potential marker to identify patients with advanced adenomatous polyps, that is, precursors of colorectal cancer. © 2013 Atakan Yesil et al.
PubMed | Istanbul Medeniyet University, Haydarpasa Numune Training and Research Hospital and Duzen Laboratories
Type: | Journal: Gastroenterology research and practice | Year: 2013
Background. To investigate the relationship between DR-70 serum levels and dysplastic colon polyps. Materials and Methods. A total of 130 patients with adenomatous polyps detected by colonoscopy and divided into two groups including low versus high grade polyp, along with 50 healthy blood donors were included in the study. Blood samples from each participant were analyzed for serum CEA and DR-70 levels. Results. No statistically significant differences were observed between the two groups in terms of age or gender. The median DR-70 level was 0.5 g/mL in the healthy control group and 1.1 g/mL in group 1b (i.e., the high grade polyp) (P < 0.001). DR-70 was higher in group 1b as compared to group 1a (P < 0.001). However, the median DR-70 values for the low grade polyp group (i.e., group 1a) and the control group were similar (P = 0.067). In order to determine independent predictors of high grade dysplasia, CEA, DR-70, polyp size, and age parameters were subjected to multiple logistical regression analyses via the Enter method; the model was statistically significant (P < 0.001). Conclusions. DR-70, a marker used to measure FDP, which is generated by all major cancers, is a potential marker to identify patients with advanced adenomatous polyps, that is, precursors of colorectal cancer.