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Buenos Aires, Argentina

Katzmarzyk P.T.,Pennington Biomedical Research Center | Shen W.,Columbia University | Baxter-Jones A.,University of Saskatchewan | Bell J.D.,Imperial College London | And 12 more authors.
Pediatric Obesity | Year: 2012

The 2011 Pennington Biomedical Research Center's Scientific Symposium focused on adiposity in children and adolescents. The symposium was attended by 15 speakers and other invited experts. The specific objectives of the symposium were to (i) integrate the latest published and unpublished findings on the laboratory and clinical assessment of depot-specific adiposity in children and adolescents, (ii) understand the variation in depot-specific adiposity and related health outcomes associated with age, sex, maturation, ethnicity and other factors and (iii) identify opportunities for incorporating new markers of abdominal obesity into clinical practice guidelines for obesity in children and adolescents. This symposium provided an overview of important new advances in the field and identified directions for future research. The long-term goal of the symposium is to aid in the early identification of children and adolescents who are at increased health risk because of obesity and obesity-related conditions. © 2012 The Authors.

Wingeyer S.D.P.,Hemostasis and Thrombosis Laboratory | Graffigna M.N.,Durand Hospital | Belli S.H.,Durand Hospital | Benetucci J.,Hospital of Infectious Diseases F. J. Muniz | De Larranaga G.F.,Hemostasis and Thrombosis Laboratory
Genetic Testing and Molecular Biomarkers | Year: 2012

Aim: Plasminogen activator inhibitor-1 (PAI-1) and tumor necrosis factor-α (TNF-α) are increased in the circulation of obese persons. Because a direct link between PAI-1 and TNF-α in obesity has been observed, they are candidate genes for the development of obesity. We sought to evaluate the relation between the genotypic and allelic frequencies of the-675 4G/5G PAI-1 and-308 G/A TNF-α polymorphisms and their association with the risk for obesity in an Argentinean population. Methods: A group of 110 consecutive obese persons and a group of 111 lean controls were recruited. Polymerase chain reaction was used to determine the frequency of PAI-1 and TNF-α polymorphisms; serum fasting glucose, insulin, and lipid levels were measured by standard methods. Insulin sensitivity was evaluated by using homeostasis model assessment. Results: The-308 TNF-α and-675 4G/5G PAI-1 genotype distribution did not significantly differ between the groups (p=0.544 and p=0.327, respectively). Homeostasis model assessment was the only positive independent determinant of body mass index (R2=0.493; p<0.001). Conclusion: The-675 4G/5G PAI-1 and the-308 TNF-α polymorphism variants tested in this study, individually or combined, were not associated with obesity in an Argentinean population. © 2012 Mary Ann Liebert, Inc.

Lucero D.,University of Buenos Aires | Zago V.,University of Buenos Aires | Lopez G.I.,University of Buenos Aires | Graffigna M.,Durand Hospital | And 10 more authors.
Clinica Chimica Acta | Year: 2011

Background: Hepatic steatosis (HS) is closely associated to metabolic syndrome (MS). Both, VLDL-triglyceride oversecretion and intrahepatic deposits, can take place. We evaluated VLDL characteristics, CETP, hepatic lipase (HL), IDL and small dense LDL (sdLDL), in patients with HS associated to MS. Methods: We studied 3 groups matched by age and sex: 25 MS patients with HS (diagnosed by ultrasonography), 25 MS patients without HS and 25 healthy controls. Main measurements were: lipid profile, free fatty acids, VLDL composition, VLDL size by HPLC, CETP and HL activities, IDL-cholesterol and sdLDL-cholesterol. Results: Patients with HS presented higher triglyceride levels, HOMA-IR and free fatty acids, VLDL mass and VLDL-apoB (p < 0.05). No differences in VLDL composition were observed. MS groups presented higher proportion of large VLDL than controls (p < 0.05). HS group showed higher CETP than controls (p = 0.01) and almost higher than MS without HS (p = 0.06). CETP correlated with VLDL-cholesterol content, r = 0.48, p < 0.005. The increase in sdLDL-cholesterol correlated with CETP (r = 0.47) and HL (r = 0.56), independent of insulin resistance (p < 0.003). Conclusion: Despite intrahepatic fat, patients with HS secreted higher number of VLDL particles. CETP would have a remodeling action on VLDL in circulation, enriching it in cholesterol and also favoring, together with HL, the formation of sdLDL. © 2010 Elsevier B.V.

Lucero D.,University of Buenos Aires | Zago V.,University of Buenos Aires | Lopez G.I.,University of Buenos Aires | Graffigna M.,Durand Hospital | And 9 more authors.
Clinica Chimica Acta | Year: 2011

Background: It is not elucidated if liver fat deposits associated to metabolic syndrome (MS) aggravate the atherogenic state. We evaluated, in MS patients, if the presence of non-alcoholic hepatic steatosis (HS) determines differences in inflammatory markers and VLDL characteristics. Methods: Seventy-five patients with MS were divided into 2 groups depending on the presence or absence of HS, assessed by ultrasound. Lipid profile, free fatty acids (FFA), VLDL composition, adiponectin, tumor necrosis factor-alpha (TNF-α), high sensitivity C-reactive protein (hs-CRP), and soluble adhesion molecules (sVCAM-1 and sICAM-1) were measured. Results: HS patients presented increased triglycerides levels, HOMA-IR and FFA. Patients with HS showed a reduction in adiponectin (p= 0.04) and increase in hs-CRP (p= 0.02), independently of insulin-resistance (IR). FFA correlated positively with TNF-α (p= 0.04) and inversely with adiponectin (p= 0.01). hs-CRP correlated with all inflammatory markers, independently of IR: TNF-α (r= 0.34, p= 0.02), sVCAM-1 (r= 0.29 p= 0.03), sICAM-1 (r= 0.56, p= 0.01), adiponectin (r= -0.34, p= 0.04). HS patients presented higher VLDL mass and number of particles. Adiponectin correlated with VLDL cholesterol content (r= -0.47, p= 0.04), independently of IR. VLDL, once secreted, would suffer from changes, becoming more atherogenic. Conclusions: Simple HS would play an important role increasing cardiovascular risk, independently of IR. hs-CRP may represent a useful biomarker of this condition. © 2010 Elsevier B.V.

Hirschler V.,Durand Hospital | Merono T.,CONICET | Maccallini G.,Durand Hospital | Gomez Rosso L.,CONICET | And 2 more authors.
Archives of Medical Research | Year: 2011

Background and Aims: Cholesteryl ester transfer protein (CETP) has been proposed to be associated with high risk of cardiovascular disease. Increased CETP activity was previously reported in obese adults, although its association with lifestyle behaviors has not been assessed in healthy adolescents. We undertook this study to determine the association between CETP activity and overweight/obesity, insulin resistance markers, components of the metabolic syndrome and lifestyle behaviors in healthy adolescent males. Methods: Data were collected from 164 adolescents from an amateur rugby club. Body mass index (BMI), blood pressure (BP), Tanner stages, lipids, glucose, insulin and CETP activity were measured. Questionnaires for daily intake of breakfast, sweet drinks, milk, and hours of TV watching were completed. Results: About 26% of the adolescents were obese and 23% overweight. The prevalence of metabolic syndrome was 6.7%. CETP activity was higher in obese than in normal and overweight adolescents (174 ± 35, 141 ± 30, and 149 ± 38%/ml/min, respectively; p <0.001). Univariate correlations showed an inverse association between CETP and HDL-C (r = -0.43; p = 0.018) and positive ones with BMI (r = 0.38; p = 0.007), systolic BP (r = 0.20; p <0.01) triglycerides (r = 0.40; p = 0.001), LDL-C (r = 0.46; p <0.001), TV watching >2 h/day (r = 0.17; p 0.02), and milk intake >3 glasses/day (r = 0.16; p = 0.03). Multivariate analysis showed that triglycerides, LDL-C, HDL-C, TV watching >2 h/day, milk intake >3 glasses/day and BMI were significant independent predictors for CETP (R 2 = 0.41). Conclusions: Unhealthy lifestyle habits such as TV watching >2 h daily and milk intake higher than three glasses per day and the increase in BMI were shown to be closely associated with high CETP activity in apparently healthy adolescent males. Future longitudinal studies should be performed to confirm these findings. © 2011 IMSS.

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