Dubai Police General Head Quarters

Dubai, United Arab Emirates

Dubai Police General Head Quarters

Dubai, United Arab Emirates
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Alghafri R.,Dubai Police General Head Quarters | Zupanic Pajnic I.,University of Ljubljana | Zupanc T.,University of Ljubljana | Balazic J.,University of Ljubljana | Shrivastava P.,DNA Fingerprinting Unit
International Journal of Legal Medicine | Year: 2017

Rapidly mutating Y-chromosomal short tandem repeats (RM Y-STRs) were identified to improve differentiation of unrelated males and also to enable separating closely and distantly related males in human identity testing in forensic and other applications. RM-Yplex assay was developed as a single multiplex that is capable of simultaneously amplifying all currently known RM Y-STRs, and reproducibility and sensitivity testing were performed on reference samples. Additional analyses are necessary to test its suitability for analysing compromised forensic samples. For this purpose, we applied the RM-Yplex assay to approximately 70-year-old skeletons that were used as a model for poorly preserved, challenging forensic samples. We analysed 57 male skeletal remains (bones and teeth) from 55 skeletons excavated from the Second World War (WWII) mass graves in Slovenia. The RM-Yplex typing was successful in all 57 samples; there were 56% full profiles obtained, and in partial profiles, up to 7 locus drop-outs were observed and they appeared correlated with low DNA quantities and degradation of DNA obtained from WWII bone and tooth samples. The longest loci, DYS403S1b, DYS547, DYS627 and DYS526b, were the most often dropped-out RM Y-STRs. In spite of high frequency of drop-out events, the RM-Yplex typing was successful in all WWII samples, showing the possibility of successful amplification of at least half of the RM Y-STRs even from the most compromised samples analysed. © 2017 Springer-Verlag Berlin Heidelberg


Alghafri R.,Dubai Police General Head Quarters | Alhammadi S.,United Arab Emirates University | Amiri K.,United Arab Emirates University
Forensic Science International: Genetics Supplement Series | Year: 2015

Rapidly Mutating Y-STRs (RM Y-STR) were proven to have significantly higher resolution in worldwide populations when compared to commonly used Y-STR kits. In order to contribute to the research that has been conducted so far, RM Y-STRs were investigated in parallel with Yfiler® kit in 327 male individuals from United Arab Emirates population. Such population is considerably isolated where Yfiler® and Powerplex® Y haplotypes were found to be shared between distantly related as well as non-related male individuals. In the present study, a comparison between Y-STR markers included in Yfiler® kit (DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385, DYS393, DYS391, DYS439, DYS635, DYS392, YGATAH4, DYS437, DYS438, DYS448) with RM Y-STRs (DYF387S1, DYF399S1, DYF403S1a/b, DYF404S1, DYS449, DYS518, DYS526a/b, DYS547, DYS570, DYS576, DYS612, DYS626, and DYS627) is conducted. RM Y-STRs were analysed using previously published methods. Forensic parameters were calculated for each set of markers, including discrimination capacity, haplotype diversity and gene diversity, using Arlequin v3.5 software. © 2015 Elsevier Ireland Ltd.


Alghafri R.,Dubai Police General Head Quarters
Forensic Science International: Genetics Supplement Series | Year: 2015

The high power of discrimination for SE33 locus makes it one of the most informative loci for forensic applications. Considering that locus finding its way to most of the new commercially available autosomal STR multiplexes assays, it is important to evaluate forensic parameters in UAE population before implementing kits including such locus. Here we present a genetic population study for SE33 locus in the United Arab Emirates (UAE) Arab population. 588 randomly collected samples were amplified using optimized in-house singleplex reaction. Forensic parameters including matching probability, power of discrimination, paternity power of exclusion and typical paternity index were calculated using Arlequin v3.5 software. Results demonstrate SE33 competence in UAE population as same as in other populations. In comparison with previously published data of 15 autosomal STR loci for UAE population [1], SE33 locus was found to have the highest power of discrimination and therefore it demonstrates the potential usefulness for forensic applications in caseworks as well as in complex paternity cases. © 2015 Elsevier Ireland Ltd


Alghafri R.,Dubai Police General Head Quarters
Forensic Science International: Genetics Supplement Series | Year: 2015

Multi-allelic Y chromosome short tandem repeats (Y-STRs) have been avoided in forensic applications due to high level of complexity involved in the interpretation of these markers. Because of such reason Y-STR multi-allelic markers have less representation in literature compared to Y-STR single-allelic markers. However these markers were proven to be highly polymorphic previously such as DYS464. In the recent discovery of the 13 rapidly mutating Y-STRs there were four multi-allelic markers including DYF387S1, DYF399S1, DYF403S1 and DYF404S1. In all subsequent studies these markers were always showing the highest diversity across the 13 RM Y-STRs. In this study these markers were investigated individually in 361 male individuals from United Arab Emirates. Each marker was analyzed using previously published primers sets. The potential forensic application value of these markers was evaluated using both conservative (C-type) and expanded (E-type) approaches. © 2015 Elsevier Ireland Ltd.


Alghafri R.,Dubai Police General Head Quarters | Almheiri R.,Dubai Police General Head Quarters | Lootah R.,Dubai Police General Head Quarters
Forensic Science International: Genetics Supplement Series | Year: 2015

The analysis of mini short tandem repeats (miniSTRs) is useful in various contexts of forensic DNA analysis. Main application is the identification of DNA profiles from degraded DNA samples. In the present study, previously identified autosomal miniSTR markers were incorporated in single multiplex assay in order to evaluate the use of such markers in forensic casework samples. Six autosomal miniSTR markers comprise of D12ATA63, D17S974, D9S2157, D2S1776, D10S1435 and D3S4529 were chosen based on previous performance study in addition to the FGA and amelogenine markers. Sensitivity of the assay was tested down to 31.25 pg of DNA. Allele's frequencies were established using 301 samples randomly collected from United Arab Emirates Arab population. Forensic parameters were calculated using Arlequin v3.5 software. © 2015 Elsevier Ireland Ltd.

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