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Dubai, United Arab Emirates

Alghafri R.,Dubai Police General Head Quarters | Alhammadi S.,United Arab Emirates University | Amiri K.,United Arab Emirates University
Forensic Science International: Genetics Supplement Series | Year: 2015

Rapidly Mutating Y-STRs (RM Y-STR) were proven to have significantly higher resolution in worldwide populations when compared to commonly used Y-STR kits. In order to contribute to the research that has been conducted so far, RM Y-STRs were investigated in parallel with Yfiler® kit in 327 male individuals from United Arab Emirates population. Such population is considerably isolated where Yfiler® and Powerplex® Y haplotypes were found to be shared between distantly related as well as non-related male individuals. In the present study, a comparison between Y-STR markers included in Yfiler® kit (DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385, DYS393, DYS391, DYS439, DYS635, DYS392, YGATAH4, DYS437, DYS438, DYS448) with RM Y-STRs (DYF387S1, DYF399S1, DYF403S1a/b, DYF404S1, DYS449, DYS518, DYS526a/b, DYS547, DYS570, DYS576, DYS612, DYS626, and DYS627) is conducted. RM Y-STRs were analysed using previously published methods. Forensic parameters were calculated for each set of markers, including discrimination capacity, haplotype diversity and gene diversity, using Arlequin v3.5 software. © 2015 Elsevier Ireland Ltd. Source


Alghafri R.,Dubai Police General Head Quarters
Forensic Science International: Genetics Supplement Series | Year: 2015

Multi-allelic Y chromosome short tandem repeats (Y-STRs) have been avoided in forensic applications due to high level of complexity involved in the interpretation of these markers. Because of such reason Y-STR multi-allelic markers have less representation in literature compared to Y-STR single-allelic markers. However these markers were proven to be highly polymorphic previously such as DYS464. In the recent discovery of the 13 rapidly mutating Y-STRs there were four multi-allelic markers including DYF387S1, DYF399S1, DYF403S1 and DYF404S1. In all subsequent studies these markers were always showing the highest diversity across the 13 RM Y-STRs. In this study these markers were investigated individually in 361 male individuals from United Arab Emirates. Each marker was analyzed using previously published primers sets. The potential forensic application value of these markers was evaluated using both conservative (C-type) and expanded (E-type) approaches. © 2015 Elsevier Ireland Ltd. Source


Alghafri R.,Dubai Police General Head Quarters | Almheiri R.,Dubai Police General Head Quarters | Lootah R.,Dubai Police General Head Quarters
Forensic Science International: Genetics Supplement Series | Year: 2015

The analysis of mini short tandem repeats (miniSTRs) is useful in various contexts of forensic DNA analysis. Main application is the identification of DNA profiles from degraded DNA samples. In the present study, previously identified autosomal miniSTR markers were incorporated in single multiplex assay in order to evaluate the use of such markers in forensic casework samples. Six autosomal miniSTR markers comprise of D12ATA63, D17S974, D9S2157, D2S1776, D10S1435 and D3S4529 were chosen based on previous performance study in addition to the FGA and amelogenine markers. Sensitivity of the assay was tested down to 31.25 pg of DNA. Allele's frequencies were established using 301 samples randomly collected from United Arab Emirates Arab population. Forensic parameters were calculated using Arlequin v3.5 software. © 2015 Elsevier Ireland Ltd. Source

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