Leibniz Insitut DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH

Braunschweig, Germany

Leibniz Insitut DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH

Braunschweig, Germany
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Carro L.,University of Salamanca | Pukall R.,Leibniz Insitut DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH | Sproer C.,Leibniz Insitut DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH | Kroppenstedt R.M.,Leibniz Insitut DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH | Trujillo M.E.,University of Salamanca
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2013

A filamentous actinomycete strain designated CR18T was isolated on humic acid agar from the rhizosphere of a Pisum sativum plant collected in Spain. This isolate was observed to grow optimally at 28 C, pH 7.0 and in the presence of 5 % NaCl. Phylogenetic analyses based on the 16S rRNA gene sequence indicated a close relationship with the type strains of Micromonospora chersina and Micromonospora endolithica. A further analysis based on a concatenated DNA sequence stretch of 4,523 bp that included partial sequences of the atpD, gyrB, recA, rpoB and 16S rRNA genes clearly differentiated the new strain from recognized Micromonospora species compared. DNA-DNA hybridization studies further supported the taxonomic position of strain CR18T as a novel genomic species. Chemotaxonomic analyses which included whole cell sugars, polar lipids, fatty acid profiles and menaquinone composition confirmed the affiliation of the new strain to the genus Micromonospora and also highlighted differences at the species level. These studies were finally complemented with an array of physiological tests to help differentiate between the new strain and its phylogenetic neighbours. Consequently, strain CR18T (= CECT 7890T = DSM 45598T) is proposed as the type strain of a novel species, Micromonospora halotolerans sp. nov. © 2013 Springer Science+Business Media Dordrecht.


Carro L.,University of Salamanca | Riesco R.,University of Salamanca | Sproer C.,Leibniz Insitut DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH | Trujillo M.E.,University of Salamanca
Systematic and Applied Microbiology | Year: 2016

Three novel actinobacterial strains, GUI2T, GUI42 and CR21 isolated from nodular tissues and the rhizosphere of a sweet pea plant collected in Cañizal, Spain were identified according to their 16S rRNA gene sequences as new members of the genus Micromonospora. The closest phylogenetic members were found to be Micromonospora saelicesensis (99.2%) "Micromonospora zeae" (99.1%), "Micromonospora jinlongensis" (99%), Micromonospora lupini (98.9%) and Micromonospora zamorensis (98.8%). To resolve their full taxonomic position, four additional genes (atpD, gyrB, recA, rpoB) were partially sequenced and compared to available Micromonospora type strain sequences. DNA-DNA hybridization, BOX-PCR and ARDRA profiles confirmed that these strains represent a novel genomic species. All strains contained meso-diaminopimelic and hydroxy-diaminopimelic acids in their cell wall. Their fatty acid profiles comprised iso-C15:0, iso-C16:0 and anteiso-C15:0 as major components. The polar lipids diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were found in the type strain GUI2T which also contained MK-10(H4) as the major menaquinone. Physiological and biochemical characteristics also differentiated the new isolates. Based on the integration of the above studies, strains GUI2T, GUI42 and CR21 represent a novel Micromonospora species and we propose the name Micromonospora luteifusca sp. nov. The type strain is GUI2T (=CECT 8846T; =DSM 100204T). © 2016 Elsevier GmbH.


PubMed | Leibniz Insitut DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH and University of Salamanca
Type: Journal Article | Journal: Systematic and applied microbiology | Year: 2016

Three novel actinobacterial strains, GUI2(T), GUI42 and CR21 isolated from nodular tissues and the rhizosphere of a sweet pea plant collected in Caizal, Spain were identified according to their 16S rRNA gene sequences as new members of the genus Micromonospora. The closest phylogenetic members were found to be Micromonospora saelicesensis (99.2%) Micromonospora zeae (99.1%), Micromonospora jinlongensis (99%), Micromonospora lupini (98.9%) and Micromonospora zamorensis (98.8%). To resolve their full taxonomic position, four additional genes (atpD, gyrB, recA, rpoB) were partially sequenced and compared to available Micromonospora type strain sequences. DNA-DNA hybridization, BOX-PCR and ARDRA profiles confirmed that these strains represent a novel genomic species. All strains contained meso-diaminopimelic and hydroxy-diaminopimelic acids in their cell wall. Their fatty acid profiles comprised iso-C15:0, iso-C16:0 and anteiso-C15:0 as major components. The polar lipids diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were found in the type strain GUI2(T) which also contained MK-10(H4) as the major menaquinone. Physiological and biochemical characteristics also differentiated the new isolates. Based on the integration of the above studies, strains GUI2(T), GUI42 and CR21 represent a novel Micromonospora species and we propose the name Micromonospora luteifusca sp. nov. The type strain is GUI2(T) (=CECT 8846(T); =DSM 100204(T)).

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