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Gutierrez M.C.,University of Seville | Steinbuchel A.,University of Munster | Tindall B.J.,DSMZ Deutsche Sammlung fur Mikroorganismen und Zellkulturen GmbH | Rehm B.H.A.,Massey University
International Journal of Systematic and Evolutionary Microbiology | Year: 2010

Strain 56T was isolated from a hypersaline soil in Aswan (Egypt). Cells were pleomorphic rods. The organism was neutrophilic, motile and required at least 1.7 M (10% w/v) NaCl, but not MgCl2, for growth; optimal growth occurred at ≥3.8 M (≥22.5 %) NaCl. The strain was thermotolerant with an optimum temperature for growth of 40 6C, although growth was possible up to 55 °C. The G+C content of the DNA of the novel strain was 67.1 mol%. 16S rRNA gene sequence analysis revealed that strain 56T was a member of the phyletic group defined by the family Halobacteriaceae, showing the highest similarity to Halopiger xanaduensis SH-6T (99 %) and the next highest similarity of 94% to other members of the family Halobacteriaceae. DNA-DNA hybridization revealed 27% relatedness between strain 56T and Hpg. xanaduensis SH-6T. Polarlipid analysis revealed the presence of the bis-sulfated glycolipid S2-DGD-1 as the sole glycolipid and the absence of the glycerol diether analogue phosphatidylglycerosulfate. Both C20 · 20 and C 20 · 25 core lipids were present. Strain 56T accumulated large amounts of polyhydroxybutyrate and also secreted an exopolymer. Physiological and biochemical differences suggested that Hpg. xanaduanesis and strain 56T were sufficiently different to be separated into two distinct species. It is suggested that strain 56T represents a novel species of the genus Halopiger, for which the name Halopiger aswanensis sp. nov. is proposed. The type strain is strain 56T (=DSM 13151T=JCM 11628T). © 2010 IUMS. Source


Margesin R.,University of Innsbruck | Schumann P.,DSMZ Deutsche Sammlung fur Mikroorganismen und Zellkulturen GmbH | Zhang D.-C.,University of Innsbruck | Redzic M.,University of Innsbruck | And 3 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2012

A Gram-stain-positive, aerobic, non-motile, psychrophilic bacterium, designated strain Cr6-08T, was isolated from alpine glacier cryoconite. Growth of strain Cr6-08T occurred at 1-25 6C. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain Cr6-08T is most closely related to members of the genus Arthrobacter. Strain Cr6-08T possessed chemotaxonomic properties consistent with those of the genus Arthrobacter, such as peptidoglycan type A3a (L-Lys-L-Ala4), MK-9(H2) as major menaquinone and anteiso- and iso-branched compounds (anteiso-C15: 0 and iso-C15: 0) as major cellular fatty acids. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown glycolipid and three unknown polar lipids. The genomic DNA G+C content of strain Cr6-08T was 57.3 mol%. On the basis of phenotypic and chemotaxonomic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strain Cr6-08T is considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter cryoconiti sp. nov. is proposed. The type strain is Cr6-08T (5DSM 23324T 5LMG 26052T 5CGMCC 1.10698T). © 2012 IUMS. Source


Zhang D.-C.,University of Innsbruck | Schumann P.,DSMZ Deutsche Sammlung fur Mikroorganismen und Zellkulturen GmbH | Redzic M.,University of Innsbruck | Zhou Y.-G.,CAS Institute of Microbiology | And 3 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2012

A Gram-positive, non-motile, rod-shaped, psychrophilic actinomycete, designated strain Cr7-14T, was isolated from alpine glacier cryoconite. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Cr7-14T was related to members of the genus Nocardioides and shared highest 16S rRNA gene sequence similarities with the type strains of Nocardioides furvisabuli (98.6%), Nocardioides ganghwensis (98.2%), Nocardioides oleivorans (98.1%) and Nocardioides exalbidus (97.6%). The predominant cellular fatty acids of strain Cr7-14T were C17: 1v8c (39.5%) and iso-C16: 0 (32.4%). The major menaquinone was MK-8(H4). The diagnostic diamino acid in the cell-wall peptidoglycan was LL-2,6-diaminopimelic acid. The predominant cell-wall sugars were galactose and rhamnose. The polar lipid pattern contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, four unknown glycolipids and two unknown polar lipids. The genomic DNA G+C content was 71.9 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, a novel species, Nocardioides alpinus sp. nov., is proposed, with Cr7-14T (=DSM 23325T=LMG 26053T=CGMCC 1.10697T) as the type strain. © 2012 IUMS. Source


Zhang D.-C.,University of Innsbruck | Schumann P.,DSMZ Deutsche Sammlung fur Mikroorganismen und Zellkulturen GmbH | Liu H.-C.,CAS Institute of Microbiology | Xin Y.-H.,CAS Institute of Microbiology | And 3 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2010

An aerobic, Gram-reaction-positive, non-motile, psychrophilic bacterium, designated strain S6-3T, was isolated from alpine soil. Cells exhibited a rod-coccus growth cycle and produced a yellow pigment. Growth occurred at 1-25°C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S6-3T was related to members of the genus Arthrobacter, sharing highest sequence similarities with the type strains of Arthrobacter psychrolactophilus (97.9 %) and Arthrobacter stackebrandtii (97.6 %). Strain S6-3T had MK-9(H2) as the major menaquinone and anteiso-C15 : 0 as the major fatty acid. The cell-wall peptidoglycan was of type A3α L-Lys-L-Thr-Ala3. The predominant cell-wall sugars were galactose and rhamnose. The genomic DNA G+C content of strain S6-3T was 61.9 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strain S6-3T is considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter alpinus sp. nov. is proposed. The type strain is S6-3 T (=DSM 22274T =CGMCC 1.8950T). © 2010 IUMS. Source


Zhang D.-C.,University of Innsbruck | Schumann P.,DSMZ Deutsche Sammlung fur Mikroorganismen und Zellkulturen GmbH | Liu H.-C.,CAS Institute of Microbiology | Xin Y.-H.,CAS Institute of Microbiology | And 3 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2010

A Gram-stain-positive, aerobic bacterium, designated strain BZ41 T, was isolated from hydrocarbon-contaminated soil. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ41T was related to members of the genus Agromyces and showed highest similarity with the type strain of Agromyces ramosus (96.8 %). The morphological, biochemical and chemotaxonomic characteristics of the new isolate were consistent with the description of the genus Agromyces. The cell-wall peptidoglycan of strain BZ41T was of type B2γ and contained the amino acids 2,4-diaminobutyric acid, alanine, glycine and glutamic acid in an approximate molar ratio of 1.8:0.7:1.1:1.0. The predominant cell-wall sugars were galactose, glucose, mannose and rhamnose. Strain BZ41T had MK-12 and MK-11 as major menaquinones and contained anteiso-C15:0 and anteiso-C 17:0 as major fatty acids. The genomic DNA G+C content of strain BZ41T was 69.7 mol%. On the basis of phenotypic characteristics and genotypic analysis, strain BZ41T is considered to represent a novel species of the genus Agromyces, for which the name Agromyces bauzanensis sp. nov. is proposed. The type strain is BZ41T (=DSM 22275T = CGMCC 1.8984T). © 2010 IUMS. Source

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