Balabanyan V.,Drugs Technology LLC |
Ul'yanov A.,Drugs Technology LLC |
Bojat V.,Drugs Technology LLC |
Khomenko A.,RAS Research Center Kurchatov Institute |
And 4 more authors.
Russian Journal of Biopharmaceuticals | Year: 2013
A nanoparticulate (105 ± 10 nm) paclitaxel (97-98% entrapment) formulation based on lac-tic-glycolic acids copolymer was obtained via homogenization. Scanning electronic and atomic force microscopy were used for nanoparticles visualization. In vitro experiment results outline a potent cytotoxic effect of nanoparticulate paclitaxel formulation against human T-lymphoblastic leukemia cells, Jurkat WT. In vivo experiments revealed anticancer efficiency of nanoparticulate paclitaxel formulation against P-glycoprotein expressing mammary adenocarcinoma Ca755 in C57BL6 mice.
Tsarkova A.S.,Russian National Research Medical University |
Dubinnyi M.A.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry |
Baranov M.S.,Russian National Research Medical University |
Petushkov V.N.,Russian Academy of Sciences |
And 3 more authors.
Mendeleev Communications | Year: 2015
Total synthesis of AsLn2, a luciferin analogue isolated from the Siberian bioluminescent earthworm F. heliota, was performed from (Z)-5-(2,3-dimethoxy-3-oxoprop-1-en-1-yl)-2-hydroxybenzoic acid in six steps. © 2015 Mendeleev Communications. Published by ELSEVIER B.V.
PubMed | Drugs Technology LLC, Mendeleev University of Chemical Technology and Saint Petersburg State University
Type: | Journal: Journal of pharmacological and toxicological methods | Year: 2017
Capillary zone electrophoresis (CZE) was used for determination of rifabutin (RFB), an anti-tuberculosis antibiotic drug, in various pharmaceutical formulations. Apart from that, simultaneous determination of RFB and human serum albumin (HSA) was performed. Electrophoretic behaviour of RFB was examined at various pH levels. CE conditions: a quartz capillary tube (internal diameter 75mm, effective length 50cm, total length 60cm), the capillary temperature was 25, the voltage applied to the capillary tube was +20kV, the UV detection wavelength was 214nm, hydrodynamic injection of the sample was performed at 30mbar for 5s, tetraborate buffer solution (0.01, 9.2). The obtained results are characterized by high efficiency (number of theoretical plates up to 260,000) and sufficient sensitivity (LOQ starting from 0.02g/ml for RFB). The obtained data are in good accord with both HPLC results (for RFB) and spectrophotometry (for HSA).
Smolenkov A.D.,Moscow State University |
Chernobrovkina A.V.,Moscow State University |
Smirnov R.S.,Moscow State University |
Chernobrovkin M.G.,Drugs Technology LTD |
Shpigun O.A.,Moscow State University
International Journal of Environmental Analytical Chemistry | Year: 2013
A new high-performance liquid chromatography (HPLC) method for the sensitive simultaneous determination of hydrazine (Hy), monomethylhydrazine (MMH) and 1,1-dimethylhydrazine (UDMH) based upon the derivatization of hydrazines with naphthalene-2,3-dialdehyde and the separation of the derivatives on Zorbax Eclipse AAA column in a single chromatographic run under acidic conditions (pH 2.4) was developed. Hydrazine and monomethylhydrazine derivatives were found to be strongly fluorescent at λex = 273 nm, λem = 500 nm. It was shown that UDMH derivative can be detected as non-fluorescent hydrazone at 290 nm by UV-detection. Limits of detection were 0.05 μg · L-1 for Hy and MMH, and 1 μg · L-1 for UDMH for the injection volume of 100 μL. The method was validated for water sample analysis. It proved to be selective, accurate and precise with the supplementary advantage of the simple and rapid sample preparation. © 2013 Copyright Taylor and Francis Group, LLC.
Balaban'Yan V.Yu.,Drugs Technology Ltd. |
Ul'Yanov A.M.,Drugs Technology Ltd. |
Elizarova O.S.,Russian Academy of Medical Sciences |
Solev I.N.,Russian Academy of Medical Sciences |
And 3 more authors.
Russian Journal of General Chemistry | Year: 2013
The research on the nansomal forms s of low-sialylated recombinant human erythropoietin (ls-rhEPO) gave evidence for the efficiency of the nanotechnological approach to targeted delivery of this protein to the brain of experimental animals. Nanosomal ls-rhEPO formulations were found to exhibit neuroprotector activity. The established ability of the nanosomal formulations of ls-rhEPO to enhance BDNF and NGF gene expression in animal brain is to a great extent responsible for the mechanism of the neuroprotector action of this protein. © 2013 Pleiades Publishing, Ltd.