Drugs Technology LTD
Drugs Technology LTD
Ermolenko Y.,Mendeleev University of Chemical Technology |
Anshakova A.,Mendeleev University of Chemical Technology |
Osipova N.,Drugs Technology LLC |
Kamentsev M.,Saint Petersburg State University |
And 3 more authors.
Journal of Pharmacological and Toxicological Methods | Year: 2017
Capillary zone electrophoresis (CZE) was used for determination of rifabutin (RFB), an anti-tuberculosis antibiotic drug, in various pharmaceutical formulations. Apart from that, simultaneous determination of RFB and human serum albumin (HSA) was performed. Electrophoretic behaviour of RFB was examined at various pH levels. CE conditions: a quartz capillary tube (internal diameter 75 mm, effective length 50 cm, total length 60 cm), the capillary temperature was 25 °С, the voltage applied to the capillary tube was + 20 kV, the UV detection wavelength was 214 nm, hydrodynamic injection of the sample was performed at 30 mbar for 5 s, tetraborate buffer solution (0.01 М, рН 9.2). The obtained results are characterized by high efficiency (number of theoretical plates up to 260,000) and sufficient sensitivity (LOQ starting from 0.02 μg/ml for RFB). The obtained data are in good accord with both HPLC results (for RFB) and spectrophotometry (for HSA). © 2017 Elsevier Inc.
Malinovskaya Y.,Drugs Technology LLC |
Melnikov P.,Russian National Research Medical University |
Baklaushev V.,Federal Biomedical Agency of the Russian Federation |
Gabashvili A.,Russian National Research Medical University |
And 8 more authors.
International Journal of Pharmaceutics | Year: 2017
The paramount problem in the therapy of brain tumors is the inability of most drugs to cross the blood–brain barrier. PLGA nanoparticles overcoated with poloxamer 188 could overcome this problem and enabled a high anti-tumoral effect against the very aggressive intracranial 101.8 glioblastoma in rats that closely resembles human grade IV glioblastomas. The basis for the transport of these particles across the blood–brain barrier appears to be adsorption of blood apolipoproteins (ApoE or ApoA-I) on the nanoparticle surface caused by the poloxamer 188-coating, followed by receptor-mediated transcytosis of the nanoparticles. The objective of the present study is the elucidation of the mechanism by which the poloxamer 188-coated nanoparticles then enter the brain tumor cells. Their intracellular fate, therefore, was investigated using the U87 human glioma cell line. The main mechanism of the PLGA nanoparticle internalization by U87 cells was clathrin-mediated endocytosis. Within 1 h free doxorubicin was released from late endosomes and could reach its target site, i.e. the DNA in the nuclei without degradation, whereas the PLGA nanoparticles, which were labeled with Cy5.5, still were observed in the endo-lysosomal compartment. These results demonstrate that the underlying mechanism of action in the brain cells is by diffusive doxorubicin release from the nanoparticles rather than by their intracellular degradation. © 2017 Elsevier B.V.
Balabanyan V.,Drugs Technology LLC |
Ul'yanov A.,Drugs Technology LLC |
Bojat V.,Drugs Technology LLC |
Khomenko A.,RAS Research Center Kurchatov Institute |
And 4 more authors.
Russian Journal of Biopharmaceuticals | Year: 2013
A nanoparticulate (105 ± 10 nm) paclitaxel (97-98% entrapment) formulation based on lac-tic-glycolic acids copolymer was obtained via homogenization. Scanning electronic and atomic force microscopy were used for nanoparticles visualization. In vitro experiment results outline a potent cytotoxic effect of nanoparticulate paclitaxel formulation against human T-lymphoblastic leukemia cells, Jurkat WT. In vivo experiments revealed anticancer efficiency of nanoparticulate paclitaxel formulation against P-glycoprotein expressing mammary adenocarcinoma Ca755 in C57BL6 mice.
Tsarkova A.S.,Russian National Research Medical University |
Dubinnyi M.A.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry |
Baranov M.S.,Russian National Research Medical University |
Petushkov V.N.,Russian Academy of Sciences |
And 3 more authors.
Mendeleev Communications | Year: 2015
Total synthesis of AsLn2, a luciferin analogue isolated from the Siberian bioluminescent earthworm F. heliota, was performed from (Z)-5-(2,3-dimethoxy-3-oxoprop-1-en-1-yl)-2-hydroxybenzoic acid in six steps. © 2015 Mendeleev Communications. Published by ELSEVIER B.V.
PubMed | Drugs Technology LLC, Mendeleev University of Chemical Technology and Saint Petersburg State University
Type: | Journal: Journal of pharmacological and toxicological methods | Year: 2017
Capillary zone electrophoresis (CZE) was used for determination of rifabutin (RFB), an anti-tuberculosis antibiotic drug, in various pharmaceutical formulations. Apart from that, simultaneous determination of RFB and human serum albumin (HSA) was performed. Electrophoretic behaviour of RFB was examined at various pH levels. CE conditions: a quartz capillary tube (internal diameter 75mm, effective length 50cm, total length 60cm), the capillary temperature was 25, the voltage applied to the capillary tube was +20kV, the UV detection wavelength was 214nm, hydrodynamic injection of the sample was performed at 30mbar for 5s, tetraborate buffer solution (0.01, 9.2). The obtained results are characterized by high efficiency (number of theoretical plates up to 260,000) and sufficient sensitivity (LOQ starting from 0.02g/ml for RFB). The obtained data are in good accord with both HPLC results (for RFB) and spectrophotometry (for HSA).
Smolenkov A.D.,Moscow State University |
Chernobrovkina A.V.,Moscow State University |
Smirnov R.S.,Moscow State University |
Chernobrovkin M.G.,Drugs Technology LTD |
Shpigun O.A.,Moscow State University
International Journal of Environmental Analytical Chemistry | Year: 2013
A new high-performance liquid chromatography (HPLC) method for the sensitive simultaneous determination of hydrazine (Hy), monomethylhydrazine (MMH) and 1,1-dimethylhydrazine (UDMH) based upon the derivatization of hydrazines with naphthalene-2,3-dialdehyde and the separation of the derivatives on Zorbax Eclipse AAA column in a single chromatographic run under acidic conditions (pH 2.4) was developed. Hydrazine and monomethylhydrazine derivatives were found to be strongly fluorescent at λex = 273 nm, λem = 500 nm. It was shown that UDMH derivative can be detected as non-fluorescent hydrazone at 290 nm by UV-detection. Limits of detection were 0.05 μg · L-1 for Hy and MMH, and 1 μg · L-1 for UDMH for the injection volume of 100 μL. The method was validated for water sample analysis. It proved to be selective, accurate and precise with the supplementary advantage of the simple and rapid sample preparation. © 2013 Copyright Taylor and Francis Group, LLC.
Balaban'Yan V.Yu.,Drugs Technology Ltd. |
Ul'Yanov A.M.,Drugs Technology Ltd. |
Elizarova O.S.,Russian Academy of Medical Sciences |
Solev I.N.,Russian Academy of Medical Sciences |
And 3 more authors.
Russian Journal of General Chemistry | Year: 2013
The research on the nansomal forms s of low-sialylated recombinant human erythropoietin (ls-rhEPO) gave evidence for the efficiency of the nanotechnological approach to targeted delivery of this protein to the brain of experimental animals. Nanosomal ls-rhEPO formulations were found to exhibit neuroprotector activity. The established ability of the nanosomal formulations of ls-rhEPO to enhance BDNF and NGF gene expression in animal brain is to a great extent responsible for the mechanism of the neuroprotector action of this protein. © 2013 Pleiades Publishing, Ltd.