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Kumar A.,Sanjay Gandhi Post Graduate Institute of Medical Sciences | Bala L.,Sanjay Gandhi Post Graduate Institute of Medical Sciences | Kalita J.,Sanjay Gandhi Post Graduate Institute of Medical Sciences | Misra U.K.,Sanjay Gandhi Post Graduate Institute of Medical Sciences | And 3 more authors.
Clinica Chimica Acta | Year: 2010

Background: Amyotrophic lateral sclerosis (ALS), an invariably fatal neurological disorder shows complicated pathogenesis that poses challenges with respect to diagnosis as well as monitoring of disease progression. Methods: We investigated metabolite profiles in the serum of 30 patients with ALS, 10 patients of Hirayama disease, which served as a neurological disease control and 25 healthy controls by using (1) H NMR spectroscopy. Results: Compared to healthy controls, the ALS patients had higher quantities of glutamate (P<0.001), beta-hydroxybutyrate (P<0.001), acetate (P<0.01), acetone (P<0.05), and formate (P<0.001), and lower concentrations of glutamine (P<0.02), histidine (P<0.001) and N-acetyl derivatives. On the other hand, Hirayama disease patients had significantly higher median concentrations of pyruvate (P<0.05), glutamate (P<0.001), formate (P<0.05) and lower median concentrations of N-acetyl derivatives. Furthermore, we also found that serum glutamate showed a positive correlation (P<0.001, r=0.6487) whereas, histidine showed a negative correlation (P<0.001, r=-0.5641) with the duration of the disease in ALS. Conclusions: Such (1) H NMR study of serum may reveal abnormal metabolite patterns, which could have the potential to serve as surrogate markers for monitoring ALS disease progression. © 2009 Elsevier B.V. Source


Pathak A.,Indian Institute of Toxicology Research | Shanker R.,Indian Institute of Toxicology Research | Garg S.K.,Dr Rml Avadh University | Manickam N.,Indian Institute of Toxicology Research
Applied Microbiology and Biotechnology | Year: 2011

We have developed an oligonucleotide microarray for the detection of biodegradative genes and bacterial diversity and tested it in five contaminated ecosystems. The array has 60-mer oligonucleotide probes comprising 14,327 unique probes derived from 1,057 biodegradative genes and 880 probes representing 110 phylogenetic genes from diverse bacterial communities, and we named it as BiodegPhyloChip. The biodegradative genes are involved in the transformation of 133 chemical pollutants. Validation of the microarray for its sensitivity specificity and quantitation were performed using DNA isolated from well-characterized mixed bacterial cultures also having non-target strains, pure degrader strains, and environmental DNA. Application of the developed array using DNA extracted from five different contaminated sites led to the detection of 186 genes, including 26 genes unique to the individual sites. Hybridization of 16S rRNA probes revealed the presence of bacteria similar to well-characterized genera involved in biodegradation of various pollutants. Genes involved in complete degradation pathways for hexachlorocyclohexane (lin), 1,2,4-trichlorobenzene (tcb), naphthalene (nah), phenol (mph), biphenyl (bph), benzene (ben), toluene (tbm), xylene (xyl), phthalate (pht), Salicylate (sal), and resistance to mercury (mer) were detected with highest intensity. The most abundant genes belonged to the enzyme hydroxylases, monooxygenases, and dehydrogenases which were present in all the five samples. Thus, the array developed and validated here shall be useful in assessing not only the biodegradative potential but also the composition of environmentally useful bacteria, simultaneously, from hazardous ecosystems. © 2011 Springer-Verlag. Source


Mishra S.S.,Dr Rml Avadh University | Mishra P.P.,Dr Rml Avadh University
Computers and Mathematics with Applications | Year: 2011

There always exists a significant time lag between production and consumption of the inventory items and in this lag producers face the risk of decision making not only about levels of production and consumption but also deterioration of the items that take place during the lag. In order to provide a scientific foundation to decision making about optimal quantity and price of the items, a (Q,R) model is here studied for the fuzzified deterioration occurring in time lag between production and consumption of items that deals with the traditional cobweb phenomenon and an attractive policy of permissible delay in the payment. A computational algorithm has been developed to solve the problem in order to attain the optimal quantity and its price for the model. The paper also presents the sensitivity analysis and comparative study of this model under crisp and fuzzy environments with the help of illustrative examples to simply gain the better perspectives of the model from the application point of view. © 2010 Elsevier Ltd. All rights reserved. Source


Gupta V.K.,Indian Institute of Technology Roorkee | Gupta V.K.,Dr Rml Avadh University | Atar N.,Dumlupinar University | Yola M.L.,Sinop University | And 6 more authors.
Journal of Colloid and Interface Science | Year: 2013

This study represents a novel template demonstration of a glucose biosensor based on mercaptophenyl boronic acid (MBA) terminated Ag@AuNPs/graphene oxide (Ag@AuNPs-GO) nanomaterials. The nanocomposites were characterized by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD) method. The TEM image shows that Ag@AuNPs in the nanocomposite is in the range of diameters of 10-20. nm. The nanocomposite was used for the determination of glucose through the complexation between boronic acid and diol groups of glucose. Thus, a novel glucose biosensor was further fabricated by immobilizing glucose oxidase (GOD) into MBA terminated Ag@AuNPs-GO nanocomposite film (MBA-Ag@AuNPs-GO). The linearity range of glucose was obtained as 2-6. mM with detection limit of 0.33. mM. The developed biosensor was also applied successfully for the determination of glucose in blood samples. The concentration value of glucose in blood samples was calculated to be 1.97 ± 0.002. mM from measurements repeated for six times. © 2013 Elsevier Inc. Source


Singh J.,Dr Rml Avadh University | Gautam S.,Dr Rml Avadh University | Pant A.B.,Indian Institute of Toxicology Research
Cellular and Molecular Biology | Year: 2012

The survival of Antarctic flora under ozone depletion depends on their ability to acclimate against increasing UV-B radiation by employing photo protective mechanisms either by avoiding or repairing UV-B damage. A fifteen days experiment was designed to study moss (Bryum argenteum) and lichen (Umbilicaria aprina) under natural UV-B exposure and under UV filter frames at the Maitri region of Schirmacher oasis, East Antarctica. Changes in UV absorbing compounds, phenolics, carotenoids and chlorophyll content were studied for continuous fifteen days and significant changes were observed in the UV exposed plants of B. argenteum and U. aprina. The change in the UV absorbing compounds was more significant in B. argenteum (P<0.0001) than U. aprina (P<0.0002). The change in phenolic contents and total carotenoid content was significant (P<0.0001) in both B. argenteum and lichen U. aprina indicating that the increase in UV absorbing compounds, phenolic contents and total carotenoid content act as a protective mechanism against the deleterious effect of UV-B radiations. © 2012. Source

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