Dr. Ram Manohar Lohia Avadh University

Faizabad, India

Avadh University or Awadh University known as Ram Manohar Lohia Avadh University is one of the premier universities in India. It is on NH96 in Faizabad city of Uttar Pradesh province. It was established in 1975 by the government of Uttar Pradesh. Wikipedia.

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Maurya P.K.,Dr. Ram Manohar Lohia Avadh University
Clinical Medicine and Research | Year: 2016

Subacute sclerosing panencephalitis is a fatal infectious disease of childhood caused by persistence of the measles virus in the brain. The effect of human immunodeficiency virus (HIV) co-infection on subacute sclerosing panencephalitis remains elusive and rare. We report a child who developed subacute sclerosing panencephalitis following a short latency period and a rapidly progressive course with HIV co-infection. © 2016 Marshfield Clinic Health System.

Sharma S.,Indian Institute of Toxicology Research | Singh R.L.,Dr. Ram Manohar Lohia Avadh University | Kakkar P.,Indian Institute of Toxicology Research
Food and Chemical Toxicology | Year: 2011

Oxidative stress is an important factor in drug induced hepatotoxicity and antioxidants from natural sources have potential to ameliorate it. The present study was aimed to investigate cyto-protective potential of probiotic Enterococcus lactis IITRHR1 (ElSN) and Lactobacillus acidophilus MTCC447 (LaSN) lysate against acetaminophen (APAP) induced hepatotoxicity. Cultured rat hepatocytes pretreated with ElSN/LaSN showed higher cell viability under APAP stress. Pre-treatment with ElSN, restored glutathione level and reduced ROS generation significantly which are major biomarkers of oxidative stress. It also reduced NO level, MDA formation and enhanced SOD activity. Pre-treatment with probiotic lysates significantly inhibited the translocation of pro-apoptotic protein (Bax), enhanced anti-apoptotic (Bcl-2) protein levels and prevented release of cyt c to cytosol; suggesting involvement of mitochondrial proteins in protection against APAP induced oxidative cellular damage. Loss in mitochondrial membrane potential due to APAP treatment was prevented in the presence of probiotic lysates. Protective action of ElSN/LaSN pretreatment was further supported by prevention of procaspase-3 activation, DNA fragmentation and chromatin condensation, in turn inhibiting APAP induced apoptotic cell death. The results indicate that probiotic preparations modulate crucial end points of oxidative stress induced apoptosis and may be used for management of drug induced liver injury. © 2010 Elsevier Ltd.

Jamal F.,Dr. Ram Manohar Lohia Avadh University | Pandey P.K.,Dr. Ram Manohar Lohia Avadh University | Singh D.,Dr. Ram Manohar Lohia Avadh University | Khan M.Y.,Babasaheb Bhimrao Ambedkar University
Phytochemistry Reviews | Year: 2013

Plant serine protease inhibitors are defense proteins crafted by nature for inhibiting serine proteases. Use of eco-friendly, sustainable and effective protein molecules which could halt or slow down metabolism of nutrients in pest would be a pragmatic approach in insect pest management of crops. The host-pest complexes that we observe in nature are evolutionary dynamic and inter-depend on other defense mechanisms and interactions of other pests or more generally speaking symbionts with the same host. Insects have co-evolved and adapted simultaneously, which makes it necessary to investigate serine protease inhibitors in non-host plants. Such novel serine protease inhibitors are versatile candidates with vast potential to overcome the host inhibitor-insensitive proteases. In a nutshell exploring and crafting plant serine proteinase inhibitors (PIs) for controlling pests effectively must go on. Non-host PI seems to be a better choice for coevolved insensitive proteases. Transgenic plants expressing wound inducible chimaeric PIs may be an outstanding approach to check wide spectrum of gut proteinases and overcome the phenomenon of resistance development. Thus, this article focuses on an entire array of plant serine protease inhibitors that have been explored in the past decade, their mode of action and biological implications as well as applications. © 2012 Springer Science+Business Media B.V.

Saxena U.,Dr. Ram Manohar Lohia Avadh University | Sauvaget C.,The Detection Group | Sankaranarayanan R.,The Detection Group
Asian Pacific Journal of Cancer Prevention | Year: 2012

Cervical cancer remains the most frequent cancer in women from the developing world. More than 88% of deaths occur in low-income countries, and it is predicted to climb to 91.5% by 2030. Although Pap-based screening programmes have shown to be effective in reducing the disease burden in high-resource countries, implementation and sustention of cytology-based programmes is quite challenging in low-resource settings. The present paper reviews evidence-based alternatives of screening methods, triaging algorithm, treatment of cervical precancerous lesions, and age-group at screening appropriate for low-income countries. Evidence shows that visual inspection methods using diluted acid acetic or Lugol's iodine, and HPV-DNA testing are more sensitive tests than the Pap-smear screening test. Visual inspection allows an immediate result and, when appropriate, may be immediately followed by cryotherapy, the so called "screen-and-treat" approach, achieved in a single visit, by trained nurses and midwives. Examples of cervical cancer prevention programmes in India and selected low-income countries are given.

Garg S.K.,Dr. Ram Manohar Lohia Avadh University | Tripathi M.,Dr. Ram Manohar Lohia Avadh University
Reviews of Environmental Contamination and Toxicology | Year: 2011

The potential hazards associated with industrial effluents, coupled with increasing awareness of environmental problems, have prompted many countries to limit the indiscriminate discharge of untreated wastewaters. The pulp and paper industry has been among the most significant of industrial polluters of the waterways, and therefore has been one of the industries of concern. © 2011 Springer Science+Business Media, LLC.

Garg S.K.,Dr. Ram Manohar Lohia Avadh University | Tripathi M.,Dr. Ram Manohar Lohia Avadh University | Srinath T.,Indian Institute of Science
Reviews of Environmental Contamination and Toxicology | Year: 2012

Pollution of the environment by toxic metals results largely from industrial activities, although sources such as agriculture and sewage disposal also contribute to some extent (Nriagu and Pacyna 1989). Toxic metallic species, once mobilized into the environment, tend to persist, circulate, and eventually accumulate at different trophic levels in members of the food chain. Ultimately, metal pollutants pose a serious threat to the environment, and affect plants, animals, and humans (Olson and Foster 1956; Reidske 1956; Sauter et al. 1976; Levis and Bianchi 1982; Mance 1987; Xing and Okrent 1993). Metal pollutants eventually affect ecosystem function, and impose an economic and public health burden. The problems associated with wastewater disposal in developing countries can generally be attributed to lack of adequate treatment/management policies, coupled with ineffective legislation on the part of entrusted governmental agencies (The Environmental Protection Act 1991; Oboh and Aluyor 2008). Environmental awareness has grown among consumers and industrialists in recent decades, and more recently has culminated in legal constraints being imposed on emissions; such constraints have increasingly become more strict necessitating cost-effective emission control (Gadd and White 1993). © 2012 Springer Science+Business Media, LLC.

Pandey P.K.,Dr. Ram Manohar Lohia Avadh University | Jamal F.,Dr. Ram Manohar Lohia Avadh University
Pesticide Biochemistry and Physiology | Year: 2014

A trypsin inhibitor purified from the seeds of Tamarindus indica by Sephadex G-75, DEAE-Sepharose and Trypsin-Sepharose CL-4B columns was studied for its antifeedant, larvicidal, pupicidal and growth inhibitory activities against Helicoverpa armigera larvae. Tamarindus trypsin inhibitor (TTI) exhibited inhibitory activity towards total gut proteolytic enzymes of H. armigera (~87%) and bovine trypsin (~84%). Lethal doses which caused mortality and weight reduction by 50% were 1% w/w and 0.50% w/w, respectively. IC50 of TTI against Helicoverpa midgut proteases and bovine trypsin were ~2.10 μg/ml and 1.68 μg/ml respectively. In larval feeding studies the 21 kDa Kunitz-type protein was found to retard growth and development, prolonged the larval-pupal development durations along with adversely affecting the fertility and fecundity of H. armigera. In artificial diet at 0.5% w/w TTI, the efficiency of conversion of ingested food as well as of digested food, relative growth rate, growth index declined whereas approximate digestibility, metabolic cost, relative consumption rate, consumption index and total developmental period enhanced for H. armigera larvae. These results suggest that TTI has toxic and adverse effect on the developmental physiology of H. armigera and could be useful in controlling the pest H. armigera. © 2014 Elsevier Inc..

Singh S.K.,Dr. Ram Manohar Lohia Avadh University | Tripathi V.R.,Dr. Ram Manohar Lohia Avadh University | Garg S.K.,Dr. Ram Manohar Lohia Avadh University
Process Biochemistry | Year: 2012

A thermoalkaline protease with a molecular weight of 22 kDa was purified from the Bacillus cereus SIU1 strain using a combination of Q-Sepharose and Sephadex G-75 chromatography. The kinetic analyses revealed the K m, V max and k cat to be 1.09 mg ml -1, 0.909 mg ml -1 min -1 and 3.11 s -1, respectively, towards a casein substrate. The protease was most active and stable at pH 9.0 and between a temperature range of 45-55 °C. It was fully stable at 0.0-2.0% and moderately stable at 2.5-10.0% (w/v) sodium chloride. Phenyl methyl sulfonyl fluoride, ethylene diamine tetra acetic acid and ascorbic acid were inhibitory with regard to enzyme activity, whereas cysteine, β-mercaptoethanol, calcium, magnesium, manganese and copper at concentration of 1.0 mM increased enzyme activity. Sodium dodecyl sulfate, Triton X-100, Tween 80, hydrogen peroxide and sodium perborate significantly enhanced protease activity at 0.1 and 1.0% concentrations. In the presence of 0.1 and 1.0% (w/v) detergents, the protease was fairly stable and retained 50-76% activity. Therefore, it may have a possible application in laundry formulations. An initial analysis of the circular dichroism (CD) spectrum in the ultraviolet range revealed that the protease is predominantly a β-pleated structure and a detailed structural composition showed ∼50% β-sheets. The CD-based conformational evaluation of the protease after incubation with modulators, metal ions, detergents and at different pH values, revealed that the change in the β-content directly corresponded to the altered enzyme activity. The protease combined with detergent was able to destain blood stained cloth within 30 min. © 2012 Elsevier Ltd. All rights reserved.

In this study, Bacillus cereus isolate efficiently remediated 57% PCP and 74% Cr6+ simultaneously with uptake rate of 0.65 mg Cr6+ g-1 biomass h-1 at initial 200 mg Cr6+ and 500 mg PCP l-1 concentration under optimized 0.4% glucose, 0.2% NH 4Cl, pH 7.0, 35 °C, 1.0% inoculum during 60 h incubation. Optimization of agitation (100 rpm) and aeration (0.6 vvm) in bioreactor further enhanced PCP dechlorination by ∼5.0% and Cr6+ removal 7.5%. Presence of other heavy metals variedly affected bioremediation of both the toxicants. Maximum and minimum inhibition was exhibited by mercury and lead, respectively. Out of 74% Cr6+ remediated, 90% reduced to Cr 3+, of which 52.8% was associated with cell biomass and 37.2% with culture supernatant. Maximum chromate reductase activity was evident in culture supernatant followed by cytosolic fraction and cell debris. A direct correlation existed between chromate reductase activity and reduced Cr3+ in different cell fractions. Among matrices, alginate was most suitable for biomass immobilization, which enhanced Cr6+ removal by 20.2% compared to free cells at 36 h. Gas chromatography and mass spectrometry detected 2,3,4,6-tetrachlorophenol, 2,4,6-trichlorophenol, 2,6-dichlorophenol and 6-chlorohydroxyquinol as PCP dechlorination products. Our promising strain can be efficiently employed for simultaneous bioremediation of PCP and Cr 6+ under wide environmental conditions. © 2013 Elsevier Ltd. All rights reserved.

Gaur R.,Dr. Ram Manohar Lohia Avadh University | Tiwari S.,Dr. Ram Manohar Lohia Avadh University
BMC Biotechnology | Year: 2015

Background: The rising concerns about the scarcity of fossil fuels, the emission of green house gasses and air pollution by incomplete combustion of fossil fuel have also resulted in an increasing focus on the use of cellulases to perform enzymatic hydrolysis of the lignocellulosic materials for the generation of bioethanol. The aim of this study was to isolate a potential thermo-solvent tolerant cellulase producing bacterium from natural resources, and then applied for purification and characterization. The purified enzyme was to be accessible for the bioethanol production as well as industrial exploitation (discuss in our next study). Results: It is the first instance when thermo-solvent tolerant cellulase producing bacterium was isolated from soil sample. The culture was identified as Bacillus vallismortis RG-07 by 16S rDNA sequence analysis. Bacillus vallismortis RG-07 reported maximum cellulase production from sugarcane baggase (4105 U ml-1) used as agro-waste carbon source. The cellulase enzyme produced by the Bacillus sp. was purified by (NH4)2SO4 precipitation, ion exchange and gel filtration chromatography, with overall recovery of 28.8%. The molecular weight of purified cellulase was 80 kDa as revealed by SDS-PAGE and activity gel analysis. The optimum temperature and pH for enzyme activity was determined as 65°C and 7.0 and it retained 95 and 75% of activity even at 95°C, and 9.0 respectively. The enzyme activity was enhanced in the presence of organic solvents (30%) n-dodecane, iso-octane, n-decane, xylene, toluene, n-haxane, n-butanol, and cyclohexane, after prolonged incubation (7 days). The enzyme activity was also stimulated by Ca2+, mercaptoethanol, Tween-60, and Sodium hypochloride whereas strongly inhibited by Hg. Kinetic analysis of purified enzyme showed the Km and Vmax to be 1.923 mg ml-1 and 769.230 μg ml-1 min-1, respectively. Conclusion: The unique property of solvent-thermostable-alkalophilic, nature proves the potential candidature of this isolate for current mainstream biomass conversion into fuel and other industrial process. © 2015 Gaur and Tiwari.

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