Dr Hari Singh Gour University

Sagar, India

Dr. Hari Singh Gour University , formerly and more popularly known as Sagar University or University of Saugor, is a Central University in the city of Sagar, the state of Madhya Pradesh or , India. It was formerly named "Sagar University" when founded on 18 July 1946, during the British Raj. In February 1983 the name was changed to that of Sir Hari Singh Gour, the University's Founder, by the State Government. It is the oldest university in Madhya Pradesh. Wikipedia.

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Jain K.,Dr Hari Singh Gour University | Mehra N.K.,Dr Hari Singh Gour University | Jain N.K.,Dr Hari Singh Gour University
Current Opinion in Pharmacology | Year: 2014

Nanopharmacology is a relatively newer branch of pharmacology which investigates interaction of a nanomedicine with living systems at the nanoscale level. Modern medicine is increasingly concerned with various surface modified nanocarriers, such as dendrimers, nanoparticles, carbon based nanomaterials, polymer-drug nanoconjugates, etc., which have immense therapeutic potential by target specific drug delivery, using nanoscaffolding and nanocontainers, owing to the specific physical, chemical and biological properties of these moieties that is related to their nanoscale size range. Nanopharmacology could have potential medical and pharmaceutical benefits via applications of nanotechnology in the delivery of therapeutic and diagnostic agents. Nanomaterials may be expected to find application in the cardiovascular, as well as, renal arena, in the near future. © 2014 Elsevier Ltd.

Gupta U.,Dr Hari Singh Gour University | Jain N.K.,Dr Hari Singh Gour University
Advanced Drug Delivery Reviews | Year: 2010

Development of an effective drug delivery approach for the treatment of HIV/AIDS is a global challenge. The conventional drug delivery approaches including Highly Active Anti Retroviral Therapy (HAART) have increased the life span of the HIV/AIDS patient. However, the eradication of HIV is still not possible with these approaches due to some limitations. Emergence of polymeric and non-polymeric nanotechnological approaches can be opportunistic in this direction. Polymeric carriers like, dendrimers and nanoparticles have been reported for the targeting of anti HIV drugs. The synthetic pathways as well polymeric framework create some hurdles in their successful formulation development as well as in the possible drug delivery approaches. In the present article, we have discussed the general physiological aspects of the infection along with the relevance of non-polymeric nanocarriers like liposomes, solid lipid nanoparticles (SLN), ethosomes, etc. in the treatment of this disastrous disease. © 2009 Elsevier B.V. All rights reserved.

Mehra N.K.,Dr Hari Singh Gour University | Jain N.K.,Dr Hari Singh Gour University
Journal of Drug Targeting | Year: 2013

The aim of the present study was to assess the in vitro and in vivo potential of doxorubicin-loaded, folic acid appended engineered multi-walled carbon nanotubes (DOX/FA-PEG-MWCNTs) for efficient tumor targeting. The loading efficiency was determined to be 92.0 ± 0.92 (DOX/FA-PEG-MWCNTs) in phosphate buffer solution (pH 7.4) ascribed to π-π stacking interaction. The developed nanoconjugates were evaluated for in vitro DOX release, erythrocytes toxicity, ex vivo cytotoxicity and cell uptake studies on MCF-7 (breast cancer cell line). The DOX/FA-PEG-MWCNTs nanoconjugate affords higher efficacy in tumor growth suppression due to its stealth nature and most preferentially taken up by the cultured MCF-7 through caveolae-mediated endocytosis as compared to free DOX. The in vivo studies were performed to determine the pharmacokinetics, biodistribution and antitumor efficacy on tumor bearing female Sprague Dawley rats and improved pharmacokinetics confirm the function of FA-PEG conjugated CNTs. The median survival time for tumor bearing rats treated with DOX/FA-PEG-MWCNTs (30 d) was extended very significantly as compared to free DOX (p < 0.001). The results concluded that developed water-soluble nano-conjugates might emerge as "safe and effective" nano-medicine in cancer treatment by minimizing the side effects with and Generally Regarded as Safe prominence. © 2013 Informa UK Ltd.

Gupta U.,Dr Hari Singh Gour University
International journal of pharmaceutics | Year: 2010

Dendrimers are considered versatile carriers especially for the treatment of diseases like cancer, AIDS, malaria etc. Cancer is a worldwide threat particularly in developing countries. A breakthrough research in this regard is a prime requirement. In the present study, folic acid was conjugated to fifth generation polypropylene imine (PPI) dendrimers and characterized through IR, NMR ((13)C and (1)H), ESI mass spectroscopy as well as electron microscopic studies. Doxorubicin (DOX), an effective anticancer drug, was used in the present study to develop and explore the anticancer potential of the dendrimer based formulations. DOX was loaded (approximately 26 and 65%) to the PPI dendrimers as well as folate conjugated PPI (PPI-FA) dendrimers, respectively. These ligand conjugated dendrimers displayed very less (approximately 3 and 4%, respectively, for PPI-FA and PPI-FA-DOX) hemolysis. The developed formulation PPI-FA-DOX was stable enough. In vitro drug release of the formulation was found to be faster in the acidic media than at the higher pH. The prepared formulation displayed a higher cell uptake in MCF-7 cancer cell lines as evidenced by fluorescence studies. The results suggested that, in future, folic acid conjugated PPI dendrimers may emerge as a better choice for anticancer drug targeting. 2010 Elsevier B.V. All rights reserved.

Gajbhiye V.,Dr Hari Singh Gour University | Jain N.K.,Dr Hari Singh Gour University
Biomaterials | Year: 2011

Polysorbate 80 (P80) anchored poly(propyleneimine) (PPI) dendritic nanoconjugate was developed and evaluated for targeting anti-cancer drug, docetaxel (DTX) to the brain tumor. In vitro cytotoxicity studies of free DTX, DTX-PPI and DTX-P80-PPI dendrimers were carried out using U87MG human glioblastoma cell line. The in vivo anti-cancer activity in brain tumor bearing rats revealed that DTX loaded P80 conjugated dendrimers reduced the tumor volume extremely significantly (p < 0.0001; more than 50%). The median survival time for brain tumor bearing rats treated with DTX-P80-PPI dendrimers (42 days) was extended very significantly as compared to DTX-PPI (23 days; p < 0.001), receptor blocked group (15 days; p < 0.001) and free DTX (18 days; p < 0.001). Gamma scintigraphy and biodistribution studies further confirmed the targeting efficiency and higher biodistribution of ligand conjugated dendrimer into the brain. The results concluded that the developed nanoconjugate has potential to deliver significantly higher amount of drug to brain tumor for improved therapeutic outcome. © 2011 Elsevier Ltd.

Mehra N.K.,Dr Hari Singh Gour University | Mishra V.,Dr Hari Singh Gour University | Jain N.K.,Dr Hari Singh Gour University
Biomaterials | Year: 2014

Carbon nanotubes (CNTs) have emerged as fascinating materials, exhibiting promising potential in receptor based targeting owing to their unique physicochemical properties (cell membrane penetration, high surface area and drug payload, biocompatibility, easy surface modification, photoluminescence property, and non-immunogenicity etc). The hydrophilicity, a major constrain associated with the first generation of CNTs i.e. pristine CNTs, could be overcome using functionalization techniques. In the last two decades variety of functionalized CNTs ( f-CNTs) i.e. oxidized, amidated, acylated, surfactant and biopolymer-assisted, and biomolecules modified have been developed and utilized as effective, safe, nano sized, and smart systems to deliver a wide range of bioactives in the biological system. The purpose of this review is to examine the various aspects of conjugation and associated conjugation chemistry of various targeting ligands to CNTs for their respective biomedical applications. The various biomolecules have been easily tethered to CNTs surfaces including proteins and amino acid, enzymes, nucleic acid (DNA and siRNA), aptamers, vitamins, monoclonal antibodies, peptides (NGR, RGD and Aniopep-2) and so on, for targeting purposes. © 2013 Elsevier Ltd.

Jain K.,Dr Hari Singh Gour University | Jain N.K.,Dr Hari Singh Gour University
Drug Discovery Today | Year: 2013

Leishmaniasis reveals itself in two forms, cutaneous and visceral, but the later exerts serious complications and may lead to death, if untreated. The availability of limited number of antileishmanial chemotherapeutic agents, the high cost of treatment, growing incidences of resistance to first line drugs as well as severe toxicities associated with the drugs complicate the treatment of visceral leishmaniasis. To overcome these problems, critical investigation of new therapeutic strategies with potential antileishmanial activity and good tolerability are essential. In this review we explore the different facets of novel therapeutic strategies for treatment of visceral leishmaniasis with a purpose to summarize all the possible treatment tactics, which will help scientists working in this arena to implement their research in a systematic manner. © 2013 Elsevier Ltd. All rights reserved.

Marti-Centelles V.,Jaume I University | Pandey M.D.,Jaume I University | Pandey M.D.,Dr Hari Singh Gour University | Burguete M.I.,Jaume I University | Luis S.V.,Jaume I University
Chemical Reviews | Year: 2015

The success of a given macrocyclization reaction involves a very delicate balance of many different factors. First, a proper understanding of the basic thermodynamic and kinetic concepts underlying these processes is essential in defining the strategies to obtain the targeted cyclic structures and the experimental elements to be optimized. essential element for achieving high yields in a macrocyclization process is the appropriate selection of the disconnection site. This defines the reaction used for the key step, which will clearly affect the overall process, but also delineates the nature and structure of the immediate linear precursor. The presence of structural elements, including configurational elements, able to induce a favorable folding of this linear precursor in such a way that both reactive ends approach with the proper orientation provides significant enhancements in macrocyclizations. The use of templates of very different natures is a versatile strategy to overcome the limitations of the other macrocyclization strategies. The use of templates has allowed the preparation of a variety of macrocyclic structures in better yields and usually in shorter reaction times, and often allows easier purification protocols.

Kesharwani P.,Dr Hari Singh Gour University | Gajbhiye V.,Dr Hari Singh Gour University | Jain N.K.,Dr Hari Singh Gour University
Biomaterials | Year: 2012

Increasing knowledge about molecular mechanisms of endogenous RNA interference (RNAi) and small interfering RNAs (siRNAs) has been incorporated into innovative nucleic acid medicines for treatment of diseases such as cancers. Although RNAi and siRNA have the potential to become powerful therapeutic drugs, their delivery to the target site represents a major challenge. The design and creation of nanocarriers for the safe and efficient delivery of siRNA towards their potential applications site is one of the challenging and rapidly growing areas of research since they have to overcome the commonly encountered biological barriers. In this review, we discuss the recent nanotechnological strategies for siRNA delivery by using different carriers such as liposomes, dendrimers and carbon nanotubes. © 2012 Elsevier Ltd.

Bagre A.P.,Dr Hari Singh Gour University
International journal of pharmaceutics | Year: 2013

The objective of present research work was to develop alginate coated chitosan core shell nanoparticles (Alg-CS-NPs) for oral delivery of low molecular weight heparin, enoxaparin. Chitosan nanoparticles (CS-NPs) were synthesized by ionic gelation of chitosan using sodium tripolyphosphate. Core shell nanoparticles were prepared by coating CS-NPs with alginate solution under mild agitation. The Alg-CS-NPs were characterized for surface morphology, surface coating, particle size, polydispersity index, zeta potential, drug loading and entrapment efficiency using SEM, Zeta-sizer, FTIR and DSC techniques. Alginate coating increased the size of optimized chitosan nanoparticles from around 213 nm to about 335 nm as measured by dynamic light scattering in zeta sizer and further confirmed by SEM analysis. The performance of optimized enoxaparin loaded Alg-CS-NPs was evaluated by in vitro drug release studies, in vitro permeation study across intestinal epithelium, in vivo venous thrombosis model, particulate uptake by intestinal epithelium using fluorescence microscopy and pharmacokinetic studies in rats. Coating of alginate over the CS-NPs improved the release profile of enoxaparin from the nanoparticles for successful oral delivery. In vitro permeation studies elucidated that more than 75% enoxaparin permeated across the intestinal epithelium with Alg-CS-NPs. The Alg-CS-NPs significantly increased (p<0.05) the oral bioavailability of enoxaparin in comparison to plain enoxaparin solution as revealed by threefold increase in AUC of plasma drug concentration time curve and around 60% reduction in thrombus formation in rat venous thrombosis model. The core shell Alg-CS-NPs showed promising potential for oral delivery and significantly enhanced the in vivo oral absorption of enoxaparin. Copyright © 2013 Elsevier B.V. All rights reserved.

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