Dandawate P.R.,ISTRA |
Vyas A.,ISTRA |
Vyas A.,Dr Dy Patil Biotechnology And Bioinformatics Institute |
Ahmad A.,Wayne State University |
And 8 more authors.
Pharmaceutical Research | Year: 2012
Purpose: Several formulations have been proposed to improve the systemic delivery of novel cancer therapeutic compounds, including cyclodextrin derivatives.We aimed to synthesize and characterize of CDF-β-cyclodextrin inclusion complex (1:2) (CDFCD). Methods: The compound was characterized by Fourier transform infrared, differential scanning calorimetry, powder X-ray diffraction studies, H1 & C13 NMR studies and scanning electron microscopic analysis. Its activity was tested against multiple cancer cell lines, and in vivo bioavailability was checked. Results: CDF-β-cyclodextrin was found to lower IC50 value by half when tested against multiple cancer cell lines. It preferentially accumulated in the pancreas, where levels of CDF-β-cyclodextrin in mice were 10 times higher than in serum, following intravenous administration of an aqueous CDF-β-cyclodextrin preparation. Conclusions: Novel curcumin analog CDF preferentially accumulates in the pancreas, leading to its potent anticancer activity against pancreatic cancer cells. Synthesis of such CDF-β-cyclodextrin self-assembly is an effective strategy to enhance its bioavailability and tissue distribution, warranting further evaluation for CDF delivery in clinical settings for treatment of human malignancies. © Springer Science+Business Media, LLC 2012.
Namde H.,Dr Dy Patil Biotechnology And Bioinformatics Institute |
Wani M.,Dr Dy Patil Biotechnology And Bioinformatics Institute
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2014
Tecoma stans is a tropical flowering plant which is used in herbal medicine treatment for diabetes, digestive problems etc. The present investigation was carried out to study the effect of different plant growth regulators on callus induction from Tecoma leaves and to compare the antioxidant activity from the leaves and in vitro generated callus. Qualitative analysis of phytochemicals from the leaves of T. stans were done using different solvents. Callus culture were initiated from leaf explants on Murashige and Skoog medium supplemented with different concentrations and combinations of hormones like 2,4-D, NAA, BAP and Kinetin for rapid initiation of callus and biomass production. To study the antioxidant activity the effect of leaf extract on 2, 2-diphenyl-1-picrylhdrazyl (DPPH) free radical was measured. The best efficiency of callusing was observed when 2,4-D and BAP in 2mg/l concentration each were used which was followed by 2,4-D and Kinetin. Methanolic and ethanolic leaf extract indicated higher antioxidant activity than the callus extract. In vitro studies and production of biomass and extraction of bioactive compounds may contribute in conservation management of naturally grown plants.
Jahagirdar D.,Dr Dy Patil Biotechnology And Bioinformatics Institute |
Purohit S.,Dr Dy Patil Biotechnology And Bioinformatics Institute |
Jain A.,Dr Dy Patil Biotechnology And Bioinformatics Institute |
Sharma N.K.,Dr Dy Patil Biotechnology And Bioinformatics Institute
Frontiers in Oncology | Year: 2016
Breast cancer is a leading type of cancer among women in India as well as worldwide. According to the WHO 2015 report, it has been anticipated that there would be a twofold rise in the death due to breast cancer among women. The heterogeneous property of breast carcinoma has been suggested to be linked with dedicated set of communication and signaling pathway with their surroundings, which culminate into progression and development of the cancer. Among the plethora of communication tools in the hand of breast carcinoma cells is the recently appreciated exocytosis of the tightly packed short non-coding RNA molecules, predominantly the microRNAs (miRNAs). Recent studies suggest that miRNAs may work as courier messengers to participate in endocrine and paracrine signaling to facilitate information transfer between breast carcinoma and their neighboring cells. Evidence suggests that breast tumor cells communicate via packaged miRNAs in the tumor-released microvesicles, which enrich the tumor microenvironment. There is a strong view that dissecting out the mechanistic and regulatory aspects of miRNA export and role may uncover many prospects for overcoming the signaling defects and thereby controlling aberrant cell division. The detection of circulating miRNAs associated with breast carcinoma can also be used as biomarkers for early diagnosis. This review article is an attempt to provide updated knowledge on implications of short RNAs and their transport in the breast cancer pathophysiology. © 2016 Jahagirdar, Purohit, Jain and Sharma.
Kadam K.,University of Pune |
Prabhakar P.,Dr Dy Patil Biotechnology And Bioinformatics Institute |
Jayaraman V.K.,University of Pune
Protein and Peptide Letters | Year: 2012
Bacterial lipoproteins play critical roles in various physiological processes including the maintenance of pathogenicity and numbers of them are being considered as potential candidates for generating novel vaccines. In this work, we put forth an algorithm to identify and predict ligand-binding sites in bacterial lipoproteins. The method uses three types of pocket descriptors, namely fpocket descriptors, 3D Zernike descriptors and shell descriptors, and combines them with Support Vector Machine (SVM) method for the classification. The three types of descriptors represent shape-based properties of the pocket as well as its local physio-chemical features. All three types of descriptors, along with their hybrid combinations are evaluated with SVM and to improve classification performance, WEKA-InfoGain feature selection is applied. Results obtained in the study show that the classifier successfully differentiates between ligand-binding and non-binding pockets. For the combination of three types of descriptors, 10 fold cross-validation accuracy of 86.83% is obtained for training while the selected model achieved test Matthews Correlation Coefficient (MCC) of 0.534. Individually or in combination with new and existing methods, our model can be a very useful tool for the prediction of potential ligand-binding sites in bacterial lipoproteins. © 2012 Bentham Science Publishers.
Sharma P.,Dr Dy Patil Biotechnology And Bioinformatics Institute |
Khetmalas M.B.,Dr Dy Patil Biotechnology And Bioinformatics Institute |
Tandon G.D.,Dr Dy Patil Biotechnology And Bioinformatics Institute
International Journal of Pharma and Bio Sciences | Year: 2014
Microalgae are emerging as the sustainable key source of green fuels and neutraceuticals. In the current study, a strain of Chlorella pyrenoidosa was isolated, identified and biochemically analyzed. Studies were undertaken to enhance its biomass and lipid productivities for sustainable biodiesel production. Growth was monitored in BG-11, BBM, Bristol and Fogg's medium as cell count, O.D, PCV and dry cell weight measurements. BG-11was found to be the most suitable medium. Specific growth rate was determined to be 10.64 mg/l/d in 25 days with a lipid productivity of 29.26 mg/l/d. Nitrogen stress was introduced by varying concentration of major nitrogen source in the BG-11 medium. Varied inorganic nitrogen source like urea, ammonium nitrate and ammonium sulphate had a significant effect on biomass and lipid productivities of algae. Ammonium nitrate enhanced the lipid production leading to a three fold increase in its yield. Growth rate was accelerated by 15.3 % with the supply of 5 % carbon dioxide.
Purushe S.,University of Pune |
Prakash D.,University of Pune |
Nawani N.N.,Dr Dy Patil Biotechnology And Bioinformatics Institute |
Dhakephalkar P.,Agharkar Research Institute |
Kapadnis B.,University of Pune
Bioresource Technology | Year: 2012
The present study is focused on dextranase from Streptomyces sp. NK458 with potential to remove dextran formed during sugar manufacture. The dextranase had molecular weight of 130kDa and hydrolyzed 15-25 and 410kDa dextran. Dextranase production was optimized using statistical designs and the enzyme was purified 1.8-fold with 55.5% recovery. It displayed maximum activity at pH 9.0 and 60°C and was stable over a wide range of pH from 5.0 to 10.0. The k m and V max values were 3.05mM and 17.97mmol/ml/h, respectively. Ten units of dextranase could reduce dextran content by 67% in 24h and 56% in 72h from sugarcane juice of cane variety CoS 86032. The enzyme was stable up to 3days at 30°C beyond which its activity decreased and dextran removal could be retained by supplementation of 5U of dextranase. These properties make it a promising biocatalyst for sugar industry. © 2012 Elsevier Ltd.
Grace S.V.,Dr Dy Patil Biotechnology And Bioinformatics Institute |
Murab S.,Indian Institute of Technology Delhi |
Krupadam R.J.,Indian National Environmental Engineering Research Institute
Journal of Hazardous, Toxic, and Radioactive Waste | Year: 2012
A polymer adsorbent specific for 17β-estradiol (E2) was prepared by using a molecular imprinting technique. E2 is a female hormone (i.e., estrogen) and it has been recently reported in drinking water resources. In this paper, the writers present a method for the remediation of E2 in ground water by using a molecularly imprinted polymer (MIP) adsorbent. The molecular modeling software (HyperChem 8.0) was used to select best polymer precursors for polymer preparation on the basis of interaction energy scorings. The scanning electron microscopy (SEM) and transmission electron microscopy (TEM) micrographs revealed the existence of nanopores (0.65 to 0.8 nm in diameter) in the polymer because of imprinting. The Fourier transform infrared (FT-IR) spectra of the MIP and non-imprinted polymer (NIP) further confirmed an H-bond formation between the carboxylic acid group of methacrylic acid (MAA) and E2, which was responsible for the selective adsorption of E2. The adsorption capacity and binding affinity of the MIP were determined by using Langmuir and Freundlich isotherm models. The adsorption capacity of the MIP was 96.75 mg/g this value is twice that of the commercially used activated carbon. The effect of environmental parameters [such as total dissolved solids (TDS) and pH] on the adsorption removal of E2 was studied for water and for groundwater samples collected from industrial areas. In this study, the writer report, for the first time, the reusability of an MIP adsorbent that retains its adsorption capacity for more than eight cycles. High adsorption capacity and resistance to environmental parameters such as TDS and pH demonstrated the merits of MIP over the single use of activated carbon for the remediation of E2-contaminated water. © 2012 American Society of Civil Engineers.
Karthikeyan M.,CSIR - National Chemical Laboratory |
Vyas R.,Dr Dy Patil Biotechnology And Bioinformatics Institute
Methods in Molecular Biology | Year: 2012
Efficient storage and retrieval of chemical structures is one of the most important prerequisite for solving any computational-based problem in life sciences. Several resources including research publications, text books, and articles are available on chemical structure representation. Chemical substances that have same molecular formula but several structural formulae, conformations, and skeleton framework/scaffold/functional groups of the molecule convey various characteristics of the molecule. Today with the aid of sophisticated mathematical models and informatics tools, it is possible to design a molecule of interest with specified characteristics based on their applications in pharmaceuticals, agrochemicals, biotechnology, nanomaterials, petrochemicals, and polymers. This chapter discusses both traditional and current state of art representation of chemical structures and their applications in chemical information management, bioactivity- and toxicity-based predictive studies. © 2012 Springer Science+Business Media, LLC.
Dake M.S.,Dr Dy Patil Biotechnology And Bioinformatics Institute |
Beniwal N.,Dr Dy Patil Biotechnology And Bioinformatics Institute
International Journal of Pharma and Bio Sciences | Year: 2014
Aureobasidium pullulans is ubiquitous, polymorphic and black fungus producing a variety of important metabolites including enzymes, polysaccharides and single cell protein. It is considered as a probable source for antagonistic activity against a number of phytopathogenic fungi and possible biocontrol agent of post harvest diseases. The present work aims at the antibacterial compounds produced by Aureobasidium pullulans (NCIM 1049). Extraction of antimicrobial compounds was carried out using solvent extraction and ammonium sulfate precipitation method. Antibacterial activity of resultant extracts was studied using agar disc diffusion method. Extraction using ethyl acetate as well as by salt precipitation method exhibited significant intracellular and extracellular antibacterial activity against Staphylococcus aureus, Escherichia coli and Bacillus subtilis. The antibacterial activity resulting from salt precipitation method showed stability from pH 7-9, thermostability upto 60°C. It was insensitive to SDS, EDTA and enzymes like trypsin, papain and Serratia peptidase.
Misra S.,Medical University of South Carolina |
Ghatak S.,Medical University of South Carolina |
Patil N.,University of Pune |
Dandawate P.,University of Pune |
And 5 more authors.
Bioorganic and Medicinal Chemistry | Year: 2013
Cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) enzyme have been found to play a role in promoting growth in colon cancer cell lines. The di-tert-butyl phenol class of compounds has been found to inhibit both COX-2 and 5-LOX enzymes with proven effectiveness in arresting tumor growth. In the present study, the structural analogs of 2,6 di-tert-butyl-p-benzoquinone (BQ) appended with hydrazide side chain were found to inhibit COX-2 and 5-LOX enzymes at micromolar concentrations. Molecular docking of the compounds into COX-2 and 5-LOX protein cavities indicated strong binding interactions supporting the observed cytototoxicities. The signaling interaction between endogenous hyaluronan and CD44 has been shown to regulate COX-2 activities through ErbB2 receptor tyrosine kinase (RTK) activation. In the present studies it has been observed for the first time, that three of our COX/5-LOX dual inhibitors inhibit proliferation upon hydrazide substitution and prevent the activity of pro-angiogenic factors in HCA-7, HT-29, Apc10.1 cells as well as the hyaluronan synthase-2 (Has2) enzyme over-expressed in colon cancer cells, through inhibition of the hyaluronan/CD44v6 cell survival pathway. Since there is a substantial enhancement in the antiproliferative activities of these compounds upon hydrazide substitution, the present work opens up new opportunities for evolving novel active compounds of BQ series for inhibiting colon cancer. © 2013 Elsevier Ltd. All rights reserved.