Moorthi A.,SRM University |
Saravanan S.,SRM University |
Srinivasan N.,Dr Alm Post Graduate Institute Of Basic Medical Science |
Partridge N.C.,New York University |
And 3 more authors.
Journal of Biomaterials and Tissue Engineering | Year: 2012
Current research work was aimed at evaluating the role of nano bioglass ceramic (nBGC) particles for bone formation. The nBGC particles were synthesized by sol-gel method and they were characterized by TEM, EDS, FT-IR and XRD studies. These particles were found to be non toxic to rat osteoprogenitor cells and were able to stimulate proliferation of the cells. Intracellular ERK signaling pathways as well as cell cycle gene expression (cyclin C) in rat osteoprogenitor cells were stimulated by nBGC particles. Osteoprogenitor or preosteoblastic cells were differentiated and mineralized by nBGC particles and this effect required the presence of osteogenic stimulants. Runx2, a bone specific transcription factor controlling expression of osteoblast differentiation genes was also stimulated by nBGC particles. Hence, our results suggest that nBGC particles promote bone formation by cell proliferation and differentiation, and the effect of nBGC particles on osteoblast differentiation is possibly mediated by Runx2 within the osteogenic environment. © 2012 American Scientific Publishers. All rights reserved.
Ganapathy A.,Jawaharlal Nehru Centre for Advanced Scientific Research |
Pandey N.,Jawaharlal Nehru Centre for Advanced Scientific Research |
Srisailapathy C.R.S.,Dr Alm Post Graduate Institute Of Basic Medical Science |
Jalvi R.,Ali Yavar Jung National Institute for the Hearing Handicapped |
And 10 more authors.
PLoS ONE | Year: 2014
Mutations in the autosomal genes TMPRSS3, TMC1, USHIC, CDH23 and TMIE are known to cause hereditary hearing loss. To study the contribution of these genes to autosomal recessive, non-syndromic hearing loss (ARNSHL) in India, we examined 374 families with the disorder to identify potential mutations. We found four mutations in TMPRSS3, eight in TMC1, ten in USHIC, eight in CDH23 and three in TMIE. Of the 33 potentially pathogenic variants identified in these genes, 23 were new and the remaining have been previously reported. Collectively, mutations in these five genes contribute to about one-tenth of ARNSHL among the families examined. New mutations detected in this study extend the allelic heterogeneity of the genes and provide several additional variants for structure-function correlation studies. These findings have implications for early DNA-based detection of deafness and genetic counseling of affected families in the Indian subcontinent. © 2014 Ganapathy et al.
Savarimuthu W.P.,Anna University |
Gananathan P.,Anna University |
Rao A.P.,Anna University |
Manickam E.,Dr Alm Post Graduate Institute Of Basic Medical Science |
Singaravelu G.,Anna University
Journal of Nanoscience and Nanotechnology | Year: 2015
Targeted drug delivery system using nanoparticles is a promising strategy for efficient Photodynamic therapy (PDT) as they have the potential to overcome the problems of photosensitizer and enhance the effectiveness and specificity of PDT. In this study, Protoporphyrin IX (PpIX) conjugated gold nanoparticles were synthesized using electrostatic and covalent conjugation scheme. Folic acid (FA) was also conjugated suitably to the covalent complex to vectorize the complex. Optical characterizations of the complex prove the formation of the complex. The size of the synthesized nanocomplexes was studied using light scattering measurements. The photo-toxicity of the free PpIX and PpIX-nanoparticle complexes were studied using MTT assay technique against Vero and HeLa cell lines. These In-vitro results of this study indicates that, the nanoparticle complexes are more phototoxic compared to free PpIX, with the covalent complex being the better of the two complexes and the folate-mediated nanocomplex is the superior of the studied complexes. These results ensures that nanoparticle conjugated photosensitizers equipped with FA may be an effective drug delivery mechanism for PDT. Copyright © 2015 American Scientific Publishers All rights reserved.
PubMed | Dr ALM Post Graduate Institute of Basic Medical science
Type: Journal Article | Journal: Indian journal of dermatology, venereology and leprology | Year: 2010
A total of 242 patients with clinically diagnosed tinea cruris were screened and 181 (74.7 %) were found to be positive in culture for dermatophytes. 93.9% of infections were caused by Trichophyton spp., of which 58.4% were Trichophyton rubrum, 5.5% were Epidermophyton floccosum, 3.8% were Trichophyton tonsurans and we had a single isolate of Microsporum gypseum complex. Incidence of tinea cruris was higher in males (95.6%) than in females (4.4%). 45% of the cases were recurrent and 38% of cases were chronic tinea cruris. Three patients had granulomatous lesion. Zoophilic T mentagrophytes was the major aetiologic agent isolated from all the 3 cases of granulomatous tinea cruris.