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Kyoto, Japan

Doshisha Women's College of Liberal Arts is a private women's college in Kyotanabe, Kyoto, Japan. The predecessor of the school was founded in 1876, and it was chartered as a university in 1949 Wikipedia.

Aoyama M.,Osaka University | Sun-Wada G.-H.,Doshisha Womens College of Liberal Arts | Yamamoto A.,Nagahama Institute of Bio-Science and Technology | Yamamoto M.,Osaka University | And 4 more authors.
Developmental Cell | Year: 2012

The embryonic body plan is established through positive and negative control of various signaling cascades. Late endosomes and lysosomes are thought to terminate signal transduction by compartmentalizing the signaling molecules; however, their roles in embryogenesis remain poorly understood. We showed here that the endocytic pathway participates in the developmental program by regulating the signaling activity. We modified the mouse Vam2 (mVam2) locus encoding a regulator of membrane trafficking. mVam2-deficient cells exhibited abnormally fragmented late endosomal compartments. The mutant cells could terminate signaling after the removal of the growth factors including TGF-β and EGF, except BMP-Smad1/Smad5 signaling. mVam2-deficient embryos exhibited ectopic activation of BMP signaling and disorganization of embryo patterning. We found that mVam2, which interacts with BMP type I receptor, is required for the spatiotemporal modulation of BMP signaling, via sequestration of the receptor complex in the late stages of the endocytic pathway. Source

Sun-Wada G.-H.,Doshisha Womens College of Liberal Arts | Wada Y.,Osaka University
Histology and Histopathology | Year: 2010

The vacuolar-type H+-ATPases (V-ATPases) are a family of multi-subunit ATP-dependent proton pumps involved in diverse cellular processes, including acid/base homeostasis, receptor-mediated endocytosis, processing of proteins and signaling molecules, targeting of lysosomal enzymes, and activation of various degradation enzymes. These fundamental cellular activities are naturally related to higher order physiological functions in multicellular organisms. VATPases are involved in several physiological processes, including renal acidification, bone resorption, and neurotransmitter accumulation. Both forward- and reverse-genetic approaches have revealed that V-ATPase malfunction causes diseases and/or pathophysiological states, demonstrating its diverse roles in normal physiology. Here, we focus on the recent insights into the function of mammalian V-ATPase in highly differentiated cells and tissues. Source

Kabata K.,University of Alberta | Edasawa Y.,Doshisha Womens College of Liberal Arts
Language Learning and Technology | Year: 2011

Patterns of students' language learning were examined through an asynchronous cross-cultural bilingual communication project conducted between Japanese university students learning English and Canadian university students learning Japanese. Previous studies on cross-cultural communication projects have reported positive outcomes in providing learners with opportunities for cultural and language learning. However, very few, if any, investigated whether a meaning-focused communication project like ours provides similar opportunities for language learning, and if so, what kind of language learning takes place. With focus on Canadian students' learning of Japanese, the present study addressed these questions through analyses of students' logs and message texts. The results indicated that students have opportunities for all aspects of language learning, including vocabulary, kanji, grammar, and phrase/sentential expressions. However, it was also found that the amount and type of incidental learning may depend on the students' proficiency level. Explicit corrections are noticed but often not understood unless clear explanations are given. We suggest these kinds of communication projects might further promote incidental learning opportunities by better understanding the proficiency levels of the other's second language, as well as by more and better incorporation of clear error corrections possibly accompanied by metalinguistic explanations. © 2011. Source

Pathogenic bacteria must adapt to host tissues to establish a persistent infection and cause disease. Salmonella enterica serovar Typhimurium remodels its lipopolysaccharide, including the lipid A portion, to adapt to its environment, including host tissues. This remodeling is beneficial for Salmonella because it increases resistance to antimicrobial peptides, produces a robust permeation barrier in the outer membrane, and evades host immune surveillance. Lipid A modifications are regulated at both the transcriptional level and post-translational level. Regulations at the transcriptional level are studied well, and the two-component signal transduction system PhoP-PhoQ plays a central role. Most genes involved in lipid A modifications are under the control of PhoP-PhoQ. In contrast, post-translational regulations are poorly understood, but lipid A modifications appear to affect the enzymes that catalyze other lipid A modifications. Several post-translational regulations, including lipid A deacylation by the outer membrane enzyme PagL, can occur and these complex regulations constitute an emerging notion of the complexity of lipid A modifications. This review aims to describe the role of lipid A modifications in Salmonella pathogenesis and examine how their complexity affects pathogenesis. © 2011 Elsevier Ltd. Source

Sun-Wada G.-H.,Doshisha Womens College of Liberal Arts | Wada Y.,Osaka University
Biochimica et Biophysica Acta - Bioenergetics | Year: 2015

The vacuolar H+-ATPase (V-ATPase) was first identified as an electrogenic proton pump that acidifies the lumen of intra- and extracellular compartments. The acidic pH generated by V-ATPase is important for a wide range of cellular processes as well as acidification-independent processes such as secretion and membrane fusion. In addition to these housekeeping functions, recent studies implicate V-ATPase in the direct regulation and function of signaling pathways. In this review, we describe recent findings on the functions of V-ATPase in growth regulation and tissue physiology. © 2015 Elsevier B.V. All rights reserved. Source

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