Lin G.,Sun Yat Sen University |
Wang J.,Sun Yat Sen University |
Lao X.,Sun Yat Sen University |
Li L.,Dongguan ShiLong Peoples Hospital |
And 5 more authors.
Journal of Immunotherapy | Year: 2012
The presence of regulatory T cells in patients who received therapeutic cytokine-induced killer (CIK) cells may inhibit host immunity, leading to failed immunotherapy. In this study, we investigated the impact of using interleukin-6 (IL-6) on the phenotype alteration, proliferation, and cytotoxic activity of CIK cells generated from the peripheral blood mononuclear cells of patients with hepatocellular carcinoma. We found that addition of IL-6 to CIK-cell culture medium decreased the percentage of Treg/CD4 +, Treg/CD3 +T cells in the resultant CIK cells and simultaneously increased the proliferation ability, the expression of CD45RO +CD62L lowCCR7 low effector memory phenotype, and cytotoxicity of the CIK cells against hepatocellular carcinoma in vitro. Our results also showed that the percentage of Th17/CD4 +cells was increased in CIK cells, but the proportion of Th17/CD4 +cells was not affected by the addition of IL-6 to CIK-cell culture medium. Collectively, these data suggest that IL-6 may have the potential to improve antitumor activity of CIK cells in cancer immunotherapy. Copyright © 2012 by Lippincott Williams & Wilkins. Source
Yang Y.-F.,Dongguan ShiLong Peoples Hospital |
Zhang X.-Y.,Endoscopy Center |
Yang M.,Dongguan ShiLong Peoples Hospital |
He Z.-H.,Guangxi Medical University |
And 3 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2014
Aim: Recent research suggests that nucleophosmin (NPM) may be a prognostic marker in colorectal carcinomas (CRC). We here tested its use to predict the survival of CRC patients. Methods: We investigated NPM expression by immunohistochemistry in histologically normal to malignant colorectal tissues and evaluated its association with clinicopathological variables. Overall and disease-free survival after tumor removal were calculated by the Kaplan-Meier method, and differences in survival curves were analyzed by the log-rank test. The Cox proportional hazards model was used for multivariate analysis of prognostic factors. Results: NPM expression was found significantly upregulated in CRC compared to adjacent colorectal tissue, villous adenoma, tubular adenoma and normal colorectal mucosa (p≤0.05 for all). NPM expression was statistically linked to cancer embolus, lymph node metastasis, differentiation grade, and recurrence of CRC. Overall and disease-free survival of NPM-negative CRC patients tended to be better than those for patients with NPM-positive lesions (log-rank statistic, p≤0.05 for all). Multivariate analysis indicated NPM expression as an independent prognostic indicator for CRC patients (p≤0.05 ). Conclusion: Our results suggest that NPM expression can predict the survival of CRC patients. Prognosis of CRC is determined by not only many known prognostic factors but also by NPM expression. Source