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Lin G.,Sun Yat Sen University | Wang J.,Sun Yat Sen University | Lao X.,Sun Yat Sen University | Li L.,Dongguan ShiLong Peoples Hospital | And 5 more authors.
Journal of Immunotherapy | Year: 2012

The presence of regulatory T cells in patients who received therapeutic cytokine-induced killer (CIK) cells may inhibit host immunity, leading to failed immunotherapy. In this study, we investigated the impact of using interleukin-6 (IL-6) on the phenotype alteration, proliferation, and cytotoxic activity of CIK cells generated from the peripheral blood mononuclear cells of patients with hepatocellular carcinoma. We found that addition of IL-6 to CIK-cell culture medium decreased the percentage of Treg/CD4 +, Treg/CD3 +T cells in the resultant CIK cells and simultaneously increased the proliferation ability, the expression of CD45RO +CD62L lowCCR7 low effector memory phenotype, and cytotoxicity of the CIK cells against hepatocellular carcinoma in vitro. Our results also showed that the percentage of Th17/CD4 +cells was increased in CIK cells, but the proportion of Th17/CD4 +cells was not affected by the addition of IL-6 to CIK-cell culture medium. Collectively, these data suggest that IL-6 may have the potential to improve antitumor activity of CIK cells in cancer immunotherapy. Copyright © 2012 by Lippincott Williams & Wilkins.

Yang Y.-F.,Dongguan ShiLong Peoples Hospital | Zhang X.-Y.,Endoscopy Center | Yang M.,Dongguan ShiLong Peoples Hospital | He Z.-H.,Guangxi Medical University | And 3 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2014

Aim: Recent research suggests that nucleophosmin (NPM) may be a prognostic marker in colorectal carcinomas (CRC). We here tested its use to predict the survival of CRC patients. Methods: We investigated NPM expression by immunohistochemistry in histologically normal to malignant colorectal tissues and evaluated its association with clinicopathological variables. Overall and disease-free survival after tumor removal were calculated by the Kaplan-Meier method, and differences in survival curves were analyzed by the log-rank test. The Cox proportional hazards model was used for multivariate analysis of prognostic factors. Results: NPM expression was found significantly upregulated in CRC compared to adjacent colorectal tissue, villous adenoma, tubular adenoma and normal colorectal mucosa (p≤0.05 for all). NPM expression was statistically linked to cancer embolus, lymph node metastasis, differentiation grade, and recurrence of CRC. Overall and disease-free survival of NPM-negative CRC patients tended to be better than those for patients with NPM-positive lesions (log-rank statistic, p≤0.05 for all). Multivariate analysis indicated NPM expression as an independent prognostic indicator for CRC patients (p≤0.05 ). Conclusion: Our results suggest that NPM expression can predict the survival of CRC patients. Prognosis of CRC is determined by not only many known prognostic factors but also by NPM expression.

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