Wu J.-B.,Guangzhou University |
Wu J.-B.,Dongguan Hospital of Traditional Chinese Medicine |
Ye X.-H.,Dongguan Hospital of Traditional Chinese Medicine |
Xian S.-X.,Guangzhou University |
Dong M.-G.,Dongguan Hospital of Traditional Chinese Medicine
Journal of Xi'an Jiaotong University (Medical Sciences) | Year: 2017
Objective: To investigate the effects of Xiefei Lishui recipe on left ventricle remodeling in rats with heart failure. Methods: Heart failure rat model was induced by intraperitoneal injections of doxorubicin. Rats were randomly divided into sham operation group (sham group), model group, traditional Chinese medicine group (TCM group), captopril group, and digoxin group. Distilled water, TCM [22 g/(kg·d)], captopril [19 mg/(kg·d)], and digoxin [32 μg/(kg·d)] were administered by gastrogavage in rats in different groups for 35 days, respectively. Indices of ventricle remodeling and cardiac function, plasma levels of B-type natriuretic peptide (BNP), rennin (REN), angiotensin II(AngII) and aldosterone (ALD) were measured. Cardiomyocyte apoptosis index and collagen volume fraction (CVF) were analyzed. We also assayed myocardial mRNA expressions of MMP-2/9 and TIMP-½, and their tissue inhibiting factors TIMP-1 and TIMP-2. Results: Compared with those in sham group, in model group cardiac function was significantly decreased, which could be significantly increased by TCM or captopril or digoxin, indices of cardiac remodeling were significantly increased, which could be significantly decreased by TCM or captopril (P<0.01 or P<0.05). Plasma levels of BNP, REN, AngII and ALD, cardiomyocyte apoptosis index and CVF in model group were significantly increased, could be significantly decreased by TCM or captopril (P<0.01 or P<0.05). Myocardial mRNA expressions of MMP-2, MMP-9, TIMP-1 and TIMP-2 in model group were significantly upregulated compared with those in sham group, which could be significantly downregulated by TCM (P<0.01 or P<0.05). Conclusion: Xiefei Lishui recipe can attenuate left ventricle remodeling and improve cardiac function in rats with heart failure, which may be related to downregulating myocardial mRNA expressions of MMP-2, MMP-9, TIMP-1 and TIMP-2 in the left ventricle as well as inhibiting cardiomyocyte apoptosis and myocardial fibrosis. © 2017, Editorial Board of Journal of Xi'an Jiaotong University (Medical Sciences). All right reserved.
Xian S.-X.,Guangzhou University |
Yang Z.-Q.,Guangzhou University |
Ren P.-H.,Guangzhou University |
Ye X.-H.,Dongguan Hospital of Traditional Chinese Medicine |
And 5 more authors.
Chinese Journal of Integrative Medicine | Year: 2015
Objectives: To investigate the safety and efficacy of Yangxinkang Tablets (养心康片) in patients with chronic heart failure (CHF) and syndrome of qi and yin deficiency, blood stasis, and water retention. Methods: In a double-blinded, randomized, placebo-controlled, multicenter clinical trail, 228 patients with CHF New York Heart Association (NYHA) class II or III in stage C were assigned by randomized block method to two groups in a 1:1 ratio to undergo either conventional Western treatment or conventional treatment plus Yangxinkang Tablets for 4 weeks. The outcome measure were effect of cardiac function, Chinese medicine (CM) syndromes, scores of symptoms, signs, and quality of life measured by Minnesota Living with heart failure questionnaire (MLHFQ) before and after the treatment. Results: Totally 112 patients were analyzed in the treatment group and 109 in the control group. They were comparable in NYHA functional class, basic parameters and primary diseases before treatment. Cardiac function and CM syndromes were greatly ameliorated in both groups after treatment. Total effective rates of cardiac function and CM syndrome in the treatment group were significantly higher than those in the control group (P<0.05). Total symptom score and sign score in the treatment group decreased significantly after treatment (P<0.01), which were significantly lower than those in the control group (P<0.05). There were statistically significant differences in post-treatment scores of gasp, cough with phlegm, pulmonary rales and jugular vein engorgement between the two groups (P<0.05 or P<0.01). Three MLHFQ scores decreased significantly in both groups after treatment (P<0.01). Post-treatment total scale score and physical subscale score in the treatment group and the reduction of them showed statistically significant differences (P<0.05) as compared with the control group. There was no significant difference between the two groups in emotional subscale score and the reduction after treatment (P>0.05). There was no obvious adverse reaction in either group noted during the study. Conclusions: Yangxinkang Tablets were safe and efficacious in improving cardiac function, CM syndromes, symptoms, signs, and quality of life in patients with CHF class II or III in stage C on the base of conventional treatment. © 2015, Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg.
PubMed | Yuebei Peoples Hospital, Guangzhou Hospital of Traditional Chinese Medicine, Shenzhen Hospital of Traditional Chinese Medicine, Guangzhou University and 5 more.
Type: | Journal: Journal of ethnopharmacology | Year: 2016
Shenmai injection (SMI) is a traditional Chinese herbal medicine extracted from Panax ginseng (Panax ginseng C.A. Mey, steamed and dry) and Ophiopogon japonicus (Ophiopogon japonicus (L.f.) Ker-Gawl, root). It has been widely used for the treatment of chronic heart failure (CHF) in China. However, the evidence supporting its effects remains unclear due to lack of high quality trials. The aim of this study was to investigate the efficacy and safety of SMI in CHF patients with coronary artery disease (CAD).This double-blind, multicenter study randomized 240 eligible patients equally to receive SMI or placebo (100ml/day) in addition to standard medicines for the treatment of CHF. The primary endpoint was the New York Heart Association (NYHA) functional classification. The secondary endpoints were 6-min walking distance (6MWD), short-form 36 (SF-36) hearth survey score, traditional Chinese medicines (TCM) syndrome score, left ventricular ejection fractions (LVEF) and B-type natriuretic peptide (BNP) level.During treatment of 1 week, the NYHA functional classification was gradually improved in both groups, but the SMI group demonstrated a significantly greater improvement compared with the placebo group (p=0.001). Moreover, the improvement in patients received SMI was superior to those in control group with respect to 6MWD, SF-36 score and TCM syndrome score. Treatment with SMI within 1 week was well tolerated with no apparent safety concerns.The integrative treatment with standard medicines plus SMI can further improve NYHA functional classification for patients with CHF and CAD. Therefore, SMI could be recommended in the combination therapy for CHF accompanied with CAD.
Mao Z.,Sun Yat Sen University |
Chen X.-B.,Dongguan Hospital of Traditional Chinese Medicine |
Zhong Y.-M.,Sun Yat Sen University |
Guo X.-X.,Sun Yat Sen University |
Liu X.,Sun Yat Sen University
Ophthalmic Research | Year: 2016
Background: The aim of this study is to provide a quantitative evaluation of the blood-aqueous barrier (BAB) in patients with ocular blunt trauma and evaluate its association with intraocular pressure (IOP) elevation. Methods: This is a prospective case-control study, and the following 3 groups were included: elevated IOP (45 patients with an elevated IOP secondary to ocular blunt trauma), normal IOP (27 patients with a normal IOP after ocular blunt trauma), and healthy controls. The main outcome measures were IOP and BAB function evaluated using a laser flare-cell meter (LFCM). Results: Patients had significantly higher flare intensities and cell counts than the normal controls (both p < 0.001), and the elevated-IOP group displayed even higher LFCM readings than the normal-IOP group. Aqueous flare and cell readings were positively correlated with IOP (r = 0.529 and 0.590, respectively, p < 0.001). LFCM readings in the elevated-IOP group were still significantly high even on postraumatic day 120 following anti-inflammatory treatment. Conclusion: BAB dysfunction occurred following ocular blunt trauma. Eyes with an elevated IOP displayed a more seriously disturbed BAB and a longer recovery course. Examination with a LFCM provides insight into the pathophysiology of IOP elevation and assists in making decisions concerning anti-inflammatory treatment during follow-up. © 2016 S. Karger AG, Basel. Copyright: All rights reserved.
Zang X.-H.,Southern Medical University |
Zha Z.-G.,Jinan University |
Feng Y.-H.,Dongguan Hospital of Traditional Chinese Medicine |
Gao L.-H.,Southern Medical University |
Sun H.,Southern Medical University
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010
BACKGROUND: With further understanding of deep venous thrombosis (DVT) following total hip replacement, reduction and prevention of DVT has become hot topic in clinical studies. The reports of DVT formation factors remain controversial due to small samples, little statistical significance, confusion of basic experimental and clinical results and lacks of science. OBJECTIVE: To explore the causes and factors for the early DVT following total hip replacement and summarize measures to prevent and treat early DVT to reduce incidence of complications. METHODS: A total of 1 780 cases of primary total hip replacement operation were analyzed retrospectively. The statistical indexes included sex, age, body mass, other system disease, previous hip joint operation, anesthesia, operative time, prosthetic fixation, blood transfusion, postoperative functional exercise, antithrombotics, and complication. Standardized database was built and analyzed by SPSS (version 13). Regression analysis was performed using Binary Logistic Regression. RESULTS AND CONCLUSION: Of 1 780 cases, 136 had DVT. Age, other system diseases, anesthesia, prosthetic fixation, blood transfusion, postoperative functional exercise and antithrombotics were correlated with early DVT (P < 0.05). Old age, hypertension or diabetes, general anesthesia, fixation of bone cement, whole blood transfusion were the risk factors for early DVT following total hip replacement, while postoperative functional exercise and antithrombotics were the protective factors for DVT. The incidence rate of early complications can be reduced by the methods such as dealing with perioperative treatment carefully, effectively controlling the chronic diseases, efficient evaluation before surgery, precise manipulation, and the postoperative prophylactic treatment and nursing.
PubMed | Dongguan Hospital of Traditional Chinese Medicine, Southern Medical University and Shantou Central Hospital
Type: | Journal: Chinese journal of integrative medicine | Year: 2017
To investigate the effect of salvianolic acid B (Sal B) on oxidized low-density lipoprotein (ox-LDL)-induced human umbilical vein endothelial cells (HUVECs) apoptosis and the possible mechanism.HUVECs were divided into 6 groups, including control group, ox-LDL group, vitamin C group (positive control), and 5, 10 and 20 g/mL Sal B groups. Cell viability of HUVECs was determined by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The anti-apoptotic effect of Sal B was tested by Hoechst 33258 staining and Annexin V/propidium iodide flflow cytometry analysis. Apoptosis-related genes (p53, Bcl-2 and Bax) expression and caspase-3 activity were also determined. Oxidative stress markers malondialdehyde (MDA) and superoxide dismutase (SOD) were determined by the corresponding kits.In HUVECs, ox-LDL signifificantly reduced cell viability and induced apoptosis (P<0.05 or P<0.01), however, Sal B diminished the effects of ox-LDL in a dose-dependent manner (P<0.05). Moreover, 10 and 20 g/mL Sal B reduced the expression levels of p53, increased the Bcl-2/Bax ratio and inhibited the caspase-3 activity in ox-LDL-treated HUVECs (P<0.05). In addition, 5, 10 and 20 g/mL Sal B signifificantly enhanced the activity of SOD, while decreased the level of MDA in the HUVECs which treated with ox-LDL (P<0.05).Sal B exhibited anti-apoptotic effects in ox-LDL-induced endothelial cell injury by suppressing oxidative stress, p53, and caspase-3.
Mai C.-H.,Dongguan Hospital of Traditional Chinese Medicine |
Cheng X.-G.,Beijing Jishuitan Hospital
Chinese Journal of Medical Imaging Technology | Year: 2011
Objective: To observe the image manifestations of secondary aneurysmal bone cyst (SABC). Methods: Totally 26 patients of SABC were retrospectively analyzed. The diagnosis was further evidenced by pathological findings either from open surgery or minimally invasive needle biopsy. Results: All 26 patients underwent X-ray and CT examination, expanded and lytic bony destruction were observed in all 26 cases.Fourteen lesions located at long tubular bone, 9 cases were eccentric and visible cortical bone defect was observed in 5 cases. Twenty-three cases were imaged with MR. Multiple cystic spaces were observed in 19 cases, fluid-fluid levels in 11 cases, internal septations in 9 cases and surrounding bone marrow edema was found in 7 cases. Conclusion: MRI manifestations of SABC were various and complicated, due to the variation of the original diseases. However, most lesions showed collective MRI characters of aneurysmal bone cyst. Accurate pre-operative diagnosis can be facilitated by generalizing the findings from X-ray and CT image.
He J.,Jinan University |
He J.,Dongguan Hospital of Traditional Chinese Medicine |
Dai C.,Jinan University |
Chen J.,Jinan University |
And 9 more authors.
Journal of Pure and Applied Microbiology | Year: 2013
Chlorogenic acid, a natural compound widely found in a great variety of natural products, with its wide range of potentially practical values, has been quite frequently researched in many basic scientific experiments for its biological activities, such as antimicrobial properties, and other physicochemical effects. In this study, we investigated the anti-virus role of chlorogenic acid on the toll-like receptor 7 (TLR7) signaling pathway in diverse immune cells infected with H1N1. Through the course of obtaining peripheral blood mononuclear cell(PBMC) from fresh umbilical cord blood, and stimulations by inducing factors, rhGM-CSF and rhIL-4, separately, we established a co-culture system of distinct immune cells with the medium of the H1N1 virus-infected cells(human respiratory epithelial cell). Then chlorogenic acid was added into the system as an drug intervention. The cell supernatant and immune cells were collected for RT-PCR to determine the expression levels of cytokines related to the TLR7 pathway. The results indicated that chlorogenic acid could ameliorate H1N1 virus-infected cell survival and down-regulate the expression levels of the TLR7 pathway cytokines, thus avoiding too much damage from the over-reactive immune system.
Liang H.-B.,Dongguan Hospital of Traditional Chinese Medicine |
Yu W.-H.,Dongguan Hospital of Traditional Chinese Medicine |
Tan Z.-F.,Dongguan Hospital of Traditional Chinese Medicine |
Li H.-N.,Dongguan Hospital of Traditional Chinese Medicine |
Yuan Z.-M.,Dongguan Hospital of Traditional Chinese Medicine
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010
BACKGROUND: Closed reduction and interlocking intramedullary nailing can protect blood supply of fractured bone, decrease infection and promote bone healing, which is becoming the first choice for treating femoral shaft fracture. However, it is difficult to conduct in closed reduction and keep stabilization of fractured bone. OBJECTIVE: To explore the curative effect of closed reduction and interlocking intramedullary nailing in treating femoral shaft fracture with assist of rubber tourniquet 360° elastic fixation. METHODS: From May 2008 to November 2009, 18 patients (14 males, 4 females; aged 18-65 years) with closed femoral shaft fracture were treated at the Dongguan Hospital of Traditional Chinese Medicine. Body skin was protected by cloth, elastic rubber tourniquet was doubled and 360° wrapped around the fractured bone with the length of 10 cm from up and down. Supracondylar femur was reamed by Kernig-needle; fractured bone was dealt with countertraction and horizontal rotation. Another assistant compressed the fractured bone according to the displacement of fracture, which contributes to bone fracture reduetion and interlocking intramedullary nailing. RESULTS AND CONCLUSION: All 18 patients were followed up postoperatively, no case was found with skim necrosis and damage of nerve and blood vessel. 16 cases were bone union with excellent 15 cases, fine 1 case; 2 cases were still being followed up. Callus was firstly found 1 month after operation and was obviously found in 3e months postoperatively. The result suggested that the treatment of closed reduction and interlocking intramedullary nailing with assist of rubber tourniquet 360° elastic fixation is a simple and applicable approach, which reduces the interruption of blood supply for fractured bone and closed reduction gets good result, which reduces the chance of open reduction.