Time filter

Source Type

Fengtai, China

Wu F.-X.,Shanghai University | Wu F.-X.,University of Pennsylvania | He Y.,Dongfang Hospital | Di H.-T.,Shanghai University | And 4 more authors.
PLoS ONE | Year: 2016

An optimal therapeutics to manage opioid withdrawal syndrome is desired for opioid addiction treatment. Down-regulation of endogenous endomorphin-2 (EM2) level in the central nervous system after continuous morphine exposure was observed, which suggested that increase of EM2 could be an alternative novel method for opioid dependence. As a short peptide, the short half-life of EM2 limits its clinical usage through conventional administration. In the present study, we engineered an EM2 gene using a signal peptide of mouse growth factor for an out-secretory expression of EM2 and an adenovirus as a vector, which ultimately sustained the release of EM-2. After administration of the adenovirus in central nervous system, a sustained increase of EM2 level in the cerebral spinal fluid (CSF) was observed along with a reduction of morphine withdrawal syndrome. These findings suggest that the engineered EM2 gene delivered to the central nervous system could be a novel therapeutics for withdrawal syndrome in opioid dependent subjects. © 2016 Wu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Source

Yu G.-Q.,Dongfang Hospital | Yuan W.-J.,Shanghai JiaoTong University | Cui R.-L.,Shanghai University | Fu P.,Shanghai University
Chinese Medical Journal | Year: 2010

Background: The plasma concentration of very low density lipoprotein (VLDL) is negatively correlated to renal function in glomerular diseases. Effects of VLDL on renal function have been partially attributed to the proliferation of mesangial cells. This study examined the potential role of the p42/44 mitogen activated protein kinase (MAPK) in mesangial cell proliferation induced by VLDL. Methods: Mesangial cells were treated with VLDL at different concentrations or for different time. The cell cycle of the mesangial cells was analyzed by XTT assay and flow-cytometry; MAPK activity was also assayed. In some experiments, cells were treated with VLDL together with or without 0.1 μmol/L PD 98059. Results: Ten to 500 μg/ml VLDL stimulated the proliferation of mesangial cells cultured in vitro in a concentration-dependent manner. The effect was associated with an increase in p42/44 MAPK activity. Increased proliferation of mesangial cells by VLDL was significantly attenuated by PD98059, a specific p42/44 MAPK inhibitor. Conclusion: These results indicate that the p42/44 MAPK pathway is an important regulator of mesangial cell proliferation and of renal functions. Source

Wu H.,Tongji Hospital | Wu H.,Albert Ludwigs University of Freiburg | Zhao X.,Dongfang Hospital | Fritzsche K.,Albert Ludwigs University of Freiburg | And 5 more authors.
Psychology, Health and Medicine | Year: 2014

In western countries, negative illness perceptions are associated with poor health status and affect health outcomes in primary care populations. The aim of this study is to examine the relationship between illness perception and mental and physical health status in general hospital outpatients in China. This multicentre, cross-sectional study analysed a total of 281 consecutive patients from four general hospital outpatient departments of internal medicine and traditional Chinese medicine in Beijing and Kunming. The patients answered questionnaires concerning illness perception (Brief-IPQ), somatic symptom severity (Patient Health Questionnaire-15), illness behaviour (Scale for the Assessment of Illness Behaviour), emotional distress (Hospital Anxiety and Depression Scale) and health-related quality of life (Twelve-Item Short Form Health Survey). Negative illness perception, especially negative emotional reactions, perceived illness consequences, encumbering illness concerns, and strong illness identity were significantly associated with high emotional distress, impairing illness consequences, and a low mental and physical quality of life. Using a multiple linear regression model, five strongest correlates of negative illness perception were high anxiety, seeking diagnosis verification, low mental and physical quality of life and high somatic symptom severity. The variance explained by this model was 35%. Chinese general hospital outpatients showed associations between negative illness perceptions and poor mental and physical health status that were similar to those of primary care patients in western countries. The main difference was that no association with perceived illness control was found in Chinese patients. Chinese physicians should be sensitised to their patients negative illness perceptions and should focus on helping patients cope with uncertainty and anxiety by providing an understandable illness model and increasing control beliefs. © 2013 Taylor & Francis. Source

Pan W.-R.,Xuzhou Medical College | Zeng F.-Q.,Xuzhou Medical College | Wang D.,Xuzhou Medical College | Qiu Z.-Q.,Dongfang Hospital
Lymphatic Research and Biology | Year: 2015

Purpose: To make a lymphatic specimen of the dorsum of the hand for educational and clinical purposes. Methods: A total of four hands from two unembalmed human cadavers were used. Under a surgical microscope, 6% hydrogen peroxide was employed to detect the lymphatic vessels commencing from fingers. A 30-gauge needle was inserted into vessels and injected with a barium sulphate mixture. Each specimen was radiographed, photographed, and dissected to demonstrate the lymphatic vessels on the dorsum of the hand. After being embalmed in 4% formalin for 3 months, specimen was then sealed in a plexiglass container for display. Results: An average of sixteen lymph collecting vessels were found in the subcutaneous tissue of the dorsum of the hand. The diameter of the vessels varied from 0.2 mm to 0.6 mm. Vessels were the continuation of lymph vessels arising from fingers. They travelled meanderingly in the subcutaneous tissue and traversed over or under the veins when they met. Conclusion: Actual and accurate lymphatic distributions of the dorsum of the hand have been described and displayed. The information upgrades our anatomical knowledge and the results will be of benefit for the lymphatic education and clinical application. © Mary Ann Liebert, Inc. 2015. Source

Feng X.G.,Dongfang Hospital | Xu X.J.,Dongfang Hospital | Ye S.,Shanghai JiaoTong University | Lin Y.Y.,Dongfang Hospital | And 4 more authors.
Scandinavian Journal of Rheumatology | Year: 2011

Objective: The aim of the present study was to investigate the presence of anti-Chlamydia pneumoniae (Cp) antibodies in patients with ankylosing spondylitis (AS) to determine whether there is an association with AS disease activity. Methods: Seventy-nine AS outpatients and 73 normal controls were enrolled in this casecontrol study. Serum anti-Cp immunoglobulins (CpIg) were detected by enzyme-linked immunosorbent assay (ELISA). Antibodies to EpsteinBarr virus (EBV), cytomegalovirus (CMV), and Chlamydia trachomatis (Ct) were also measured. Clinical and experimental data were collected, and the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) was determined. Patients with positive Cp IgM or Cp IgA were considered to have had a recent Cp infection. Results: Cp IgG was detected in the majority of AS patients and also controls (88.8% vs. 91.8%, respectively). The seroprevalence of Cp IgA and Cp IgM was significantly higher in AS patients than in the controls (51.9% vs. 31.5%, p = 0.010 for Cp IgA; 79.7% vs. 20.5%, p < 0.0001 for Cp IgM). Seropositivity of Cp IgM was associated with elevation of the disease activity index, including erythrocyte sedimentation rate (ESR; p = 0.021), C-reactive protein (CRP; p = 0.007) and the BASDAI (p = 0.009). Persistent positive Cp IgM was associated with active disease, while seroreversion of Cp IgM was associated with a reduction in these disease activity indices. There was no correlation between Cp IgM or Cp IgA and symptomatic upper respiratory infections or other clinical manifestations. Conclusions: Recent Cp infections occur frequently in AS patients and Cp IgM antibody is correlated with active disease. These findings indicate that Cp infections may be a triggering factor for active AS. © 2011 Informa Healthcare on behalf of The Scandinavian Rheumatology Research Foundation. Source

Discover hidden collaborations