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Asan, South Korea

Kim Y.-W.,Yonsei University | Kim Y.-W.,RH Healthcare Systems Inc | Chaturvedi P.K.,Catholic University of Korea | Chun S.N.,Dong Sung Bio Pharm Co. | And 2 more authors.
Oncology Reports | Year: 2015

Bee venom (BV) therapy is a type of alternative medical treatment used to treat various diseases in oriental medicine. The mechanisms underlying the effects of BV remain poorly understood. In the present study, we evaluated the antiviral effect of BV on cervical carcinoma cell lines (CaSki, HeLa, C33A and TC-1). BV treatments resulted in a more significant suppression of cell growth in HPV 16-infected cells (CaSki) and a lesser suppression in HPV 18-infected cells (HeLa). However, less suppression was observed in HPV-negative C33A cells. In 10 μ g/ml BV-treated CaSki cells, the mRNA expression and protein levels of HPV16 E6 and E7 were significantly decreased by BV, while HPV18 E6 and E7 mRNA expression levels were not significantly altered by 10 μ g/ml BV-treated HeLa cells. The antitumor effects of BV were in accordance with in vitro data, in restricting tumor growth in vivo and were much more effective on the suppression of tumor growth. Furthermore, the mRNA and protein expression levels of HPV16 E6 and E7 were decreased by BV in TC-1 tumors. These findings demonstrated the antiviral effects of BV in HPV-infected cervical cancer cells and the anticancer effects of BV in HPV16 E6/E7-expressed TC-1 tumors. Collectively, BV plays a differential role in suppressing HPV16-infected cells (CaSki cells) and HPV18-infected cells (HeLa cells) by the downregulation of E6/E7 protein of HPV16/18.


Jeon Y.-M.,Soonchunhyang University | Lee H.-S.,Randnter | Jeong D.,Soonchunhyang University | Oh H.-K.,Chung Nam Technopark Bio Center | And 2 more authors.
Life Sciences | Year: 2015

Aims The present study was designed to evaluate the therapeutic potential of antimicrobial photodynamic therapy (PDT) using chlorin e6 with halogen light against acne bacteria-induced inflammation. Main methods Highly purified chlorin e6 (Ce6), as a second generation photosensitizer, was synthesized from Spirulina chlorophyll. To evaluate the antimicrobial property of Ce6-mediated PDT with halogen light, the broth microdilution method and two-color fluorescence assay were used. The free radicals generated upon irradiating Ce6 with halogen light were measured using 2,7-dichlorofluorescin diacetate. Propionibacterium acnes was intradermally injected into the left ear of the ICR mice, and the anti-inflammatory effect of Ce6-mediated PDT with halogen light was measured by the histological examination. The expressions of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) as well as pro-inflammatory cytokines were also measured by Western blotting. Key findings Chlorin e6-mediated PDT with halogen light (30,000 lx) inactivated various skin bacteria, including P. acnes in a dose-dependent manner. The MIC99 value against P. acnes (KCTC3314) of Ce6 with light was > 0.49 μg/ml, whereas the MIC99 for Ce6 alone was > 31.25 μg/ml. Ce6-mediated PDT suppressed the expression of P. acnes-induced pro-inflammatory cytokines and iNOS, but not COX-2 in a mouse model. Significance This study showed a remarkable therapeutic effect of chlorin e6-mediated PDT with halogen light against P. acnes-induced inflammation. Our results suggest for the first time the potential of Ce6-mediated PDT with halogen light as a more effective and safer alternative treatment to antibiotic therapy against pathogenic infections of the skin. © 2015 Elsevier Inc.


Ryu A.-R.,Soonchunhyang University | Han C.-S.,Dong Sung Bio Pharm Co. | Oh H.-K.,Chung Nam Technopark Bio Center | Lee M.-Y.,Soonchunhyang University
Toxicology and Environmental Health Sciences | Year: 2015

Photodynamic inactivation (PDI) is based on the utilization of a photosensitizer with light source and oxygen, producing reactive oxygen species which inactivate microbes and fungi. In this study, the antimicrobial effect of PDI using chlorin e6 (Ce6) and halogen light was investigated against several microbes and fungus with different properties. The PDI effects were tested against two gram-positive bacteria, S. aureus and B. subtilis, and one gram-negative bacterium E. coli. The acne-causing P. acnes, opportunistic oral and genital pathogen C. albicans and main contributor to tooth decay of S. mutans were also used for PDI test. Notably, Ce6-mediated PDI showed superior in vitro anti-pathogenic effects on P. acnes, S. mutans, and C. albicans in this study. © 2015, Korean Society of Environmental Risk Assessment and Health Science and Springer Science+Business Media Dordrecht.


Kim J.-H.,Soonchunhyang University | Han C.-S.,Dong Sung Bio Pharm Co. | Chun S.-N.,Dong Sung Bio Pharm Co. | Lee M.-Y.,Soonchunhyang University
Toxicology and Environmental Health Sciences | Year: 2014

Photodynamic inactivation (PDI) combines a photosensitizer with light in the presence of oxygen, producing reactive oxygen species which will inactivate pathogens. The most common dermatophyte, named T. mentagrophytes, was known to cause various skin infections in human, such as dermatophytosis. In this study, the antifungal activity of chlorin e6-based PDI with halogen light for photodynamic inactivation against T. mentagrophytes was measured. We report for the first time that the chlorin e6-based PDI exhibited a significant antifungal activity against T. mentagrophytes. The use of chlorin e6 as an antifungal photosensitizer for PDI represents a prominent alternative method for treating fungal infections. © 2014, Korean Society of Environmental Risk Assessment and Health Science and Springer Science+Business Media Dordrecht.

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