Kwang Dong Pharmaceutical Co. and C.L. Pharm Corporation | Date: 2012-12-10
A method and apparatus for manufacturing an edible film are disclosed. The method includes a plurality of applying operations of applying solution for forming the edible film onto lower wrapping paper in a thin film shape. The solution-applying operations include a first solution-applying and a second solution-applying operation. Therefore, in the case of an edible medicine film product, particularly, when a tiny dosage of medicine must be loaded onto an edible medicine film, a loss of medicine can be reduced as much as possible. A variety of kinds of films can be manufactured through a multi-solution-applying operation, when different kinds of medicines, for example, those which are respectively absorbed in the mouth mucous membrane, the stomach and the intestine, are required.
Lee S.H.,Kwang Dong Pharmaceutical Co. |
Suh H.J.,Korea University |
Lee H.-S.,Korea University |
Park Y.,Korea University |
And 2 more authors.
Journal of Medicinal Food | Year: 2012
This study examined the effect of fermentation on the ability of antler to act as a stimulator of hematopoietic activity. Hemolytic anemia was induced by phenylhydrazine (PHZ) in female Sprague-Dawley rats. The vehicle or antler extract (nonfermented or fermented) mixed in drinking water was administered from Days 2 to 15 after PHZ injection. On Day 15, red blood cell counts in the fermented antler group (6.33×106/μL) were significantly higher than those in the nonfermented antler group (5.90×10 6/μL) (P<.05), and rats treated with fermented antler extract tended to have higher hemoglobin compared with rats treated with nonfermented antler extract, but not significantly. In addition, rats treated with fermented antler extract had slightly lower serum erythropoietin levels compared with nonfermented antler extract, which were not statistically different from serum erythropoietin levels of nonanemic rats. We conclude therefore that the hematopoietic activity of antler might be increased by the fermentation process. © Copyright 2012, Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition 2012.
PubMed | Catholic University of Daegu, Sungkyunkwan University, Catholic University of Korea, Korea Institute of Science and Technology and Kwang Dong Pharmaceutical Co.
Type: Journal Article | Journal: Drug metabolism and pharmacokinetics | Year: 2015
This study aimed to evaluate the potential of -cedrene as a new anti-obesity drug by characterizing absorption, metabolism and pharmacokinetics in rats. -Cedrene was administered intravenously (10 and 20 mg/kg) and orally (50 and 100 mg/kg) to female and male Sprague-Dawley rats. Blood, tissues, urine, and feces were collected at predetermined times. -Cedrene concentrations were determined by a validated gas chromatography-tandem mass spectrometry (GC-MS/MS). A gas chromatography-mass selective detection (GC-MSD) method was used to identify the major metabolite. After i.v. injection, -cedrene exhibited a rapid clearance (98.4-120.3 ml/min/kg), a large distribution volume (35.9-56.5 l/kg), and a relatively long half-life (4.0-6.4 h). Upon oral administration, it was slowly absorbed (Tmax = 4.4 h) with bioavailability of 48.7-84.8%. No gender differences were found in its pharmacokinetics. Upon oral administration, -cedrene was highly distributed to tissues, with the tissue-to-plasma partition coefficients (Kp) far greater than unity for all tissues. In particular, its distribution to lipid was notably high (Kp = 132.0) compared to other tissues. A mono-hydroxylated metabolite was identified as a preliminary metabolite in rat plasma. These results suggest that -cedrene has the favorable pharmacokinetic characteristics to be further tested as an anti-obesity drug in clinical studies.
Choi H.-S.,Seoul Womens University |
Park E.D.,Kwang Dong Pharmaceutical Co. |
Park Y.,Korea University |
Suh H.J.,Korea University
Journal of Photochemistry and Photobiology B: Biology | Year: 2015
The aim of this study is to evaluate the effect of spent coffee ground (SCG) ethanol extract on UVB-induced skin aging in hairless mice. An ethanol extract of SCG (ESCG) was prepared using the residue remaining after extraction of oil from roasted SCG. High performance liquid chromatography (HPLC) analysis showed that the content of caffeine (41.58 ± 0.54 μg/mg) was higher than that of chlorogenic acid isomers (~ 9.17 μg/mg) in ESCG. ESCG significantly decreased the UVB-induced intracellular reactive oxygen species in HaCaT cells. UVB-induced wrinkle formation in mice dorsal skin was effectively reduced by ESCG administration; high dose of ESCG (5 g/L) caused the reduction of wrinkle area by 30% compared with UVB-treated control (UVBC). This result correlated with the ESCG-mediated decrease in epidermis thickness (25%). In addition, ESCG administration significantly reduced transdermal water loss (20%) and erythema formation (35%) derived from UVB exposure. Collagen type I (COL-1) level in dorsal skin was effectively recovered by ESCG administration. These results were supported by down-regulation of collagen-degrading matrix metalloproteinase 2 (MMP2) and 9 (MMP9) expressions. Our results indicate that ESCG protects mouse skin from UVB-induced photoaging by suppressing the expression of matrix metalloproteinases. Our study suggests that ESCG may be anti-photoaging agent. © 2015 Published by Elsevier B.V.
PubMed | Korea University, Seoul Womens University and Kwang Dong Pharmaceutical Co.
Type: | Journal: Journal of photochemistry and photobiology. B, Biology | Year: 2015
The aim of this study is to evaluate the effect of spent coffee ground (SCG) ethanol extract on UVB-induced skin aging in hairless mice. An ethanol extract of SCG (ESCG) was prepared using the residue remaining after extraction of oil from roasted SCG. High performance liquid chromatography (HPLC) analysis showed that the content of caffeine (41.58 0.54 g/mg) was higher than that of chlorogenic acid isomers (~9.17 g/mg) in ESCG. ESCG significantly decreased the UVB-induced intracellular reactive oxygen species in HaCaT cells. UVB-induced wrinkle formation in mice dorsal skin was effectively reduced by ESCG administration; high dose of ESCG (5 g/L) caused the reduction of wrinkle area by 30% compared with UVB-treated control (UVBC). This result correlated with the ESCG-mediated decrease in epidermis thickness (25%). In addition, ESCG administration significantly reduced transdermal water loss (20%) and erythema formation (35%) derived from UVB exposure. Collagen type I (COL-1) level in dorsal skin was effectively recovered by ESCG administration. These results were supported by down-regulation of collagen-degrading matrix metalloproteinase 2 (MMP2) and 9 (MMP9) expressions. Our results indicate that ESCG protects mouse skin from UVB-induced photoaging by suppressing the expression of matrix metalloproteinases. Our study suggests that ESCG may be anti-photoaging agent.
Consumption of high-dose vitamin C (1250 mg per day) enhances functional and structural properties of serum lipoprotein to improve anti-oxidant, anti-atherosclerotic, and anti-aging effects via regulation of anti-inflammatory microRNA
Kim S.-M.,Yeungnam University |
Lim S.-M.,Yeungnam University |
Yoo J.-A.,Yeungnam University |
Woo M.-J.,Kwang Dong Pharmaceutical Co. |
Cho K.-H.,Yeungnam University
Food and Function | Year: 2015
Background Although the health effects of vitamin C are well known, its physiological effect on serum lipoproteins and microRNA still remain to be investigated, especially daily consumption of a high dosage. Objectives To investigate the physiological effect of vitamin C on serum lipoprotein metabolism in terms of its anti-oxidant and anti-glycation activities, and gene expression via microRNA regulation. Methods We analyzed blood parameters and lipoprotein parameters in young subjects (n = 46, 22 ± 2 years old) including smokers who consumed a high dose of vitamin C (1250 mg) daily for 8 weeks. Results Antioxidant activity of serum was enhanced with the elevation of Vit C content in plasma during 8 weeks consumption. In the LDL fraction, the apo-B48 band disappeared at 8 weeks post-consumption in all subjects. In the HDL fraction, apoA-I expression was enhanced by 20% at 8 weeks, especially in male smokers. In the lipoprotein fraction, all subjects showed significantly reduced contents of advanced glycated end products and reactive oxygen species (ROS). Triglyceride (TG) contents in each LDL and HDL fraction were significantly reduced in all groups following the Vit C consumption, suggesting that the lipoprotein was changed to be more anti-inflammatory and atherogenic properties. Phagocytosis of LDL, which was purified from each individual, into macrophages was significantly reduced at 8-weeks post-consumption of vitamin C. Anti-inflammatory and anti-senescence effects of HDL from all subjects were enhanced after the 8-weeks consumption. The expression level of microRNA 155 in HDL3 was reduced by 49% and 75% in non-smokers and smokers, respectively. Conclusion The daily consumption of a high dose of vitamin C for 8 weeks resulted in enhanced anti-senescence and anti-atherosclerotic effects via an improvement of lipoprotein parameters and microRNA expression through anti-oxidation and anti-glycation, especially in smokers. © The Royal Society of Chemistry.
Won Y.-H.,Kwang Dong Pharmaceutical Co. |
Park M.-S.,Duksung Womens University
Archives of Pharmacal Research | Year: 2010
A new series of 3-allylthio-6-(mono or disubstituted) aminopyridazines was synthesized by reacting 3-allylthio-6-chloropyridazine with several amines to develop new anticancer agents. These new compounds showed antiproliferative activities against lung cancer (A549), hepatoblastoma (Hep3b), prostate cancer (PC3), colon cancer (SW480) and cervical cancer (HeLa) cells in MTT assays, and could be promising candidates for chemotherapy of carcinomas. Compound 5 (3-allylthio-6-homopiperidinylaminopyridazine) showed higher potencies than 5-FU for inhibiting the growth of these cell lines. This suggests the potential anticancer activity of compound 5. © 2010 The Pharmaceutical Society of Korea and Springer Netherlands.
PubMed | Kwang Dong Pharmaceutical Co. and Kyung Hee University
Type: | Journal: Evidence-based complementary and alternative medicine : eCAM | Year: 2015
Kyung-Ok-Ko (KOK), a well-known traditional Korean medicinal formula, has long been used to invigorate the essential qi. This use of KOK may be associated with reproductive ability as a more modern concept. The protective effect of KOK was evaluated against deterioration of testicular function induced by heat exposure in male mice. Male fertility was disrupted by scrotal heat stress at 43C for 5 weeks. KOK (0.25, 0.50, and 2.00g/kg/day) was administered orally at 3h after the stress. To evaluate the protective effect of KOK, body weight, testicular weight, sperm count, sperm motility, and histopathological changes in the testes were evaluated. KOK-treated mice significantly recovered their general health, as evidenced by body weight. KOK-treated mice also showed significantly higher testes weights, sperm counts, and sperm motility than did the heat stress group. KOK-treated mice significantly recovered the morphological appearance of the seminiferous tubules and seminiferous epithelium. Furthermore, KOK-treated mice significantly increased antioxidant enzyme activities and reduced the protein expressions of apoptosis in the testes. KOK significantly protects against heat-induced damage to testicular function in male mice by inhibiting oxidative stress and apoptosis, indicating that KOK may be an effective agent for treatment of heat-induced male infertility.
PubMed | Kwang dong Pharmaceutical Co. and Kyung Hee University
Type: Journal Article | Journal: Phytotherapy research : PTR | Year: 2016
Hovenia dulcis Thunb. (HDT) was known to have anti-fatigue, anti-diabetes, neuroprotective, and hepatoprotective effects. In the present study, the anti-fatty liver mechanism of HDT was elucidated in oleic acid (OA)-treated Hep G2 cells and acute hyperlipidemia mouse model using Triton WR-1339. Here, HDT activated p-AMP-activated protein kinase (p-AMPK), proliferator activated receptor-, carnitine palmitoyltransferase and also inhibited the expression of lipogenesis and cholesterol synthesis proteins, such as 3-hydroxy-3-methylglutaryl-CoA reductase, sterol regulatory element binding protein-1c, SREBP-2, and fatty acid synthase in OA-treated Hep G2 cells. Conversely, AMPK inhibitor compound C blocked the anti-fatty liver effect of HDT to induce AMPK phosphorylation and decrease 3-hydroxy-3-methylglutaryl-CoA reductase and lipid accumulation by oil red O staining in OA-treated Hep G2 cells. Additionally, HDT pretreatment protected against the increase of serum total cholesterol, triglyceride, low-density lipoprotein cholesterol and phospholipid in an acute hyperlipidemia mouse model with enhancement of glutathione reductase, glutathione peroxidase, superoxide dismutase, and catalase activities. Taken together, HDT inhibits OA-induced hepatic lipid accumulation via activation of AMPK and proliferator activated receptor-/carnitine palmitoyltransferase signaling and enhancement of antioxidant activity as a potent candidate for nonalcoholic fatty liver disease and hyperlipidemia. Copyright 2016 John Wiley & Sons, Ltd.
Kwang Dong Pharmaceutical Co. | Date: 2015-04-15
Food for babies; lacteal flour for babies; medicines for sensory organs; oral contraceptive pills; anti-cough drops; protein dietetic supplements; pharmaceutical preparations for treating diabetes; pharmaceutical agents affecting metabolism; veterinary preparations for the treatment of neurodegenerative and inflammatory diseases of domestic animals and livestock; dietetic supplements for animals; pharmaceutical preparations acting on the peripheral nervous system; mineral food-supplements; pharmaceuticals for the treatment of erectile dysfunction; pharmaceutical preparations for urogenital organs; vitamin preparations; crude medicines for the relief of pain; anti-inflammatory and antipyretic preparations; pharmaceutical preparations acting on the digestive organs; cardiovascular agents for medical purposes; ophthalmic preparations; antiallergic agents for medical purposes; nutritive supplement agents for epidermis; disinfectants for sanitary purposes; mouthwashes for medical purposes; dietetic foods, namely, food supplements and nutritional supplements adapted for medical purposes; dietetic beverages, namely, beverage supplements and nutritional supplements adapted for medical purposes; medicinal herb extracts; chemical preparations for the diagnosis of pregnancy; medical nutrients, tonics and alternative preparations for the treatment of osteoporosis; cord blood for medical purposes; medical agents for tumor treatment; medical agents affecting central nervous system; medical preparations for slimming purposes; medicines for dental purposes; contact lens cleaning preparations; pharmaceutical preparations for use in dermatology; chemical contraceptives; antibiotic preparations; antipyretic analgesics; pharmaceutical preparations for treating respiratory diseases; deodorizer for fiber; microorganisms for medical purposes; first aid kits; anti-rheumatism bracelets; court plaster; sanitary pads; sanitary panties; diapers for medical purposes; diapers for babies; adhesive bands for medical purposes; herb teas for medical purposes; herb teas for medicinal purposes; diagnostic preparations for medical purposes. Germinated cereals; flour for food; processed cereals; high-protein cereal bars; cereal preparations, namely, cereal bars and high-protein cereal bars; cereal based snack food; farinaceous foods, namely, farinaceous food pastes for human consumption; ra-myun; cereal bars; hamburger sandwiches; yeast for food; confectionery, namely, candies, chocolate snack foods and chocolate; frozen yogurt confectionery ices; bread; ice cream; chocolates; instant pudding mixes; chewing gum; candies; cakes; cookies; crackers; popcorn; puddings; Korean traditional sweets and cookies; royal jelly for food purposes; natural sweetener; propolis for food purposes; rice cakes; soy sauce; compound chemical seasoning; sauces; aromatic preparations for food, namely, food flavorings and spices; salt for preserving foodstuff; tea; processed tea leaves as tea substitutes; tea substitutes; green tea; iced tea; black tea; prepared coffee; milk chocolates; coffee beverages with milk; cocoa beverages with milk; chocolate-based beverages; coffee; coffee-based beverages; cocoa; cocoa-based beverages; tea-based beverages; edible ice. Beers; water for beverages; mineral water for beverages; spring water for beverages; mineral water; vegetable juice beverages; fruit drinks and fruit juices; fruit juices; non-alcoholic beverages containing fruit juices; frozen fruit beverages; lemonades; syrups for lemonade; pastilles for effervescing fruit-based beverages; powders for effervescing fruit-based beverages; non-alcoholic fruit extracts used in the preparation of beverages; non-alcoholic fruit juice beverages; non-alcoholic beverages, namely, carbonated beverages, soft drinks, energy drinks and syrups; non-alcoholic cocktail mixes; seltzer water; soda water; soda pops; smoothies; isotonic beverages; sujeonggwa; non-alcoholic fruit extracts for beverages; fruit syrups for beverages; fruit powders for beverages; sorbets for beverages; ginseng juices; prepared beverages of vegetable and fruit; soft drinks; aerated water.