Donetsk National Medical University
Donets'k, Ukraine

Donetsk National Medical University of Maxim Gorky is one of the largest medical university of the former USSR. The University is considered as one of the best medical schools in Ukraine. Originally located in Donetsk, it was relocated to Krasnyi Lyman in 2014 due to the war in Donbass. Wikipedia.

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Whitcomb D.C.,University of Pittsburgh | Lehman G.A.,Indiana University | Vasileva G.,MHAT Rousse AD | Malecka-Panas E.,Medical University of Lódz | And 4 more authors.
American Journal of Gastroenterology | Year: 2010

Objectives: Pancreatic-enzyme replacement therapy (PERT) is the standard of care to prevent maldigestion, malnutrition, and excessive weight loss in patients with exocrine pancreatic insufficiency (EPI) due to chronic pancreatitis (CP) or pancreatic surgery (PS). Our objective was to assess the efficacy and safety of a new formulation of pancrelipase (pancreatin) delayed-release 12,000-lipase unit capsules (CREON) in patients with EPI due to CP or PS. Methods: This was a double-blind, randomized, multicountry, placebo-controlled, parallel-group trial enrolling patients 18 years old with confirmed EPI due to CP or PS conducted in clinical research centers or hospitals. After a 5-day placebo run-in period (baseline), patients were randomized to pancrelipase (72,000 lipase units per meal; 36,000 per snack) or placebo for 7 days. All patients received an individually designed diet to provide at least 100 g of fat per day. The primary efficacy measure was the change in coefficient of fat absorption (CFA) from baseline to end of the double-blind period, analyzed using non-parametric analysis of covariance. Secondary outcomes included the coefficient of nitrogen absorption (CNA), clinical symptoms, and safety parameters. Results: In total, 25 patients (median age of 54 years, 76% male) received pancrelipase and 29 patients (median age of 50 years, 69% male) received placebo. The means.d. change from baseline in CFA was significantly greater with pancrelipase vs. placebo: 32.1±18.5% vs. 8.8±12.5% (P<0.0001). Similarly, the means.±d. change from baseline in CNA was greater for pancrelipase vs. placebo: 97.78±2.3% vs. 24.4±101.0% (P=0.0013). Greater improvements from baseline in stool frequency, stool consistency, abdominal pain, and flatulence were observed with pancrelipase vs. placebo. Treatment-emergent adverse events (TEAEs) were reported in five patients (20.0%) in the pancrelipase group and in six (20.7%) in the placebo group; the most common were gastrointestinal (GI) events and metabolism/nutrition disorders. There were no treatment discontinuations due to TEAEs. Conclusions: Pancrelipase delayed-release 12,000-lipase unit capsules were effective in treating fat and nitrogen maldigestion with a TEAE rate similar to that of placebo in patients with EPI due to CP or PS. © 2010 by the American College of Gastroenterology.

Borzenko B.G.,Donetsk National Medical University
Experimental oncology | Year: 2013

A comparative study of enzyme activity features of thymidilate "salvage pathway" synthesis in blood serum and tissues of different age patients with gastric cancer (T(3-4)N(0-x)M(0)) was carried out. To evaluate the diagnostic relevance of thymidilate metabolism enzymes activities and their association with tumor growth. Enzymes activities were determined by the radioisotope method and spectrophotometrically in tumor tissues and blood serum of 74 patients. It was demonstrated that thymidine phosphorylase activity in gastric tumors is lower by 2.6 times as compared to non-neoplastic mucosa of resection margin. This being accompanied by decrease of its activity in the blood serum (from 47.9 ± 2.6 to 14.65 ± 2.4 nmol/min·mg, p < 0.001). An increase of thymidine kinase activity was revealed both in tumor tissues (more than 3.5 times) and in blood serum (from 3.9 ± 0,7 nmol/mg·h, to 6.8 ± 1.0 nmol/mg·h, p < 0.01). Changes in their activity in the postoperative period depended on the type of surgical procedure and tumor eradication. It could be suggested that control of individual dynamics of the enzymes activities in blood serum may be used as informative tool for monitoring of patients and treatment optimization.

Barinov E.F.,Donetsk National Medical University
Kardiologiya | Year: 2016

One of major complications of ischemic heart disease is myocardial infarction, which develops as a result of thrombosis at the site of ruptured atherosclerotic plaque. Platelets activation and aggregation are the key events of this process. The efficiency of aspirin and/or clopidogrel use is limited by residual platelet reactivity what indicates the need to explore its mechanisms. This review covers intracellular signaling systems involved in realization of effects of the main platelet agonists in order to specify new molecules for the target therapy in case of aspirin resistance.

Dorofeyev A.E.,Donetsk National Medical University | Vasilenko I.V.,Donetsk National Medical University | Rassokhina O.A.,Donetsk National Medical University | Kondratiuk R.B.,Donetsk National Medical University
Gastroenterology Research and Practice | Year: 2013

Background. The mucus layer in the gastrointestinal tract plays important role in host innate defense, regulation of secretion, and absorption processes, maintaining colonization resistance, which composes the integrity of protective mucus barrier in the large intestine. Investigations of mucin expression in the colon mucosa can improve the understanding of protective function of mucosal barrier in ulcerative colitis (UC) and Crohn's disease (CD). Materials and Methods. 77 patients with UC and CD were examined. Histological analysis of colon mucosa was done by standard method (haematoxylin-eosin, alcian blue at pH 1.0 and 2.5 to determine sulfated and nonsulfated glycosaminoglycans and glycoproteins, and goblet cells). To characterize the mucus production the PAS-reaction was performed. Immunohistochemistry was performed using monoclonal mouse antibodies raised against MUC2, MUC3, MUC4, and TFF3 (USBiological, USA). Results. The moderate expression of MUC2 and MUC3 (50.0% and 32.1%, P = 0.03) and high expression of MUC4 and TFF3 in the colon mucosa were observed in all patients with CD. The intensive labeling of MUC4 and TFF3 occurred more often (42.9% and 57.1%, P = 0.03) in patients with CD. The level of expression of secretory MUC2 and transmembrane MUC3 and MUC4 in all patients with UC was low, up to its complete absence (59.2% and 53.1% cases, P = 0.05). TFF3 expression had high and medium staining intensity in patients with UC. Conclusions. Different types of mucins synthesis, secretion, and expression were found in patients with UC and CD. The expression of mucin MUC2, MUC3, MUC4, and TFF3 correlated with the activity of disease and the extent of the inflammatory process in the large intestine. The most pronounced alteration of mucins expression was observed in patients with severe UC and CD. © 2013 A. E. Dorofeyev et al.

Ganna S.,Donetsk National Medical University
Revista Brasileira de Reumatologia | Year: 2014

Objectives: The aim of this study was to evaluate the prevalence of anaemia in rheumatoid arthritis (RA). Patients and methods: 89 patients who fulfilled American College of Rheumatology (ACR) criteria for RA were included in this study. The mean disease duration was 10.9±8.8 years. All patients received methotrexate (10.5±5.5 mg/week) in combination with folic acid. Steroid hormones were prescribed to 92% (19.3±3.8 mg/day) of patients. Erythrocyte sedimentation rate (ESR) and levels of hemoglobin, C-reactive protein (CRP), tumour necrosis factor-alpha (TNFa) and interleukin-1 beta (IL-1ß) were evaluated in all patients. The World Health Organization (WHO) criteria for anaemia uses a hemoglobin threshold of <120 g/L for women and <130 g/L for men. Results: Anaemia was observed in 57 (64%) of the patients (1st group), the other patients (2nd group) had normal levels of hemoglobin (135.5±10.7 g/L). Duration and activity of RA were significantly higher (p<0.05) in the 1st group compared with the 2nd. ESR, CRP, TNFa, and IL- 1ß mean levels were significantly increased (p<0.05) in the 1st group when compared with the 2nd group. Negative correlations between hemoglobin level and ESR, CRP, TNFa, and IL- 1ß concentrations were observed. Conclusion: This study showed for the first time in Ukraine that in 46% of patients with RA, anaemia was diagnosed. A reduction of hemoglobin level was associated with a high activity of disease. © 2014 Sociedade Brasileira de Reumatologia.

Gorshkov O.,Donetsk National Medical University
Journal of Statistical Mechanics: Theory and Experiment | Year: 2012

We suggest an algorithm for the estimation of the Hurst exponent that is based on the results of the well-known stabilogram diffusion analysis method of Hurst exponent estimation for one-dimensional fractals. Our algorithm can be applied to Hurst exponent estimation for fractals with two or more dimensions. To assess the efficiency of this algorithm, we compare its calculation results to those of the well-known Hurst exponent estimation detrending moving average analysis algorithm. In this paper, the computation of the Hurst exponent has been performed for two-dimensional domains of various sizes, which were generated by the Cholesky-Levinson factorization algorithm. The surrogate surfaces have Hurst exponents of H = 0.1, 0.5, and 0.9. It has been established that the detrending moving average analysis algorithm is more sensitive to high-frequency components, while the stabilogram diffusion analysis tends to be sensitive to low-frequency components. © 2012 IOP Publishing Ltd and SISSA Medialab srl.

Zuikov S.A.,Donetsk National Medical University
Advances in Gerontology | Year: 2015

The interrelationship between purine nucleotide exchange and the antioxidant system in blood plasma and erythrocytes with respect to age, as well as the contribution of key enzymes of purine decomposition in the formation of derivatives of reactive oxygen metabolites, were studied. Fifty conditionally healthy individuals aged 40–79 years were examined. An age-related increase in all indicators of the blood plasma prooxidant system was found. In blood plasma, the statistically significant correlation of the studied parameters and age of the examined individuals, as well as the relationship between glutathione peroxidase and adenosine deaminase/xanthine oxidase, adenosine deaminase and xanthine oxidase were observed. A significant increase in the content of products of protein oxidative modification was found in plasma and erythrocytes during aging. A decrease in the activity of key antioxidant enzymes, superoxide dismutase, and glutathione peroxidase with age was observed in blood plasma and erythrocytes. The data obtained are indicative of an imbalance in the antioxidant system in blood plasma and erythrocytes, with age contributing to the development of pathological states as a result of increased oxidative stress, which is greatly manifested in aging. © 2015, Pleiades Publishing, Ltd.

Zuikov S.A.,Donetsk National Medical University
Advances in Gerontology | Year: 2015

The interrelation of purine nucleotides with the antioxidant system in blood plasma and erythrocytes with respect to age, as well as the contribution of key enzymes of the purine decomposition to the formation of reactive oxygen species, were studied. Fifty conditionally healthy people aged 40–79 years (50 persons) were examined. All indicators of the pro-oxidant system of blood plasma were shown to increase with age. Statistically significant interrelations between the studied parameters in the blood plasma and age of the examined persons were found. Relationships between glutathione peroxidase and adenosine deaminase/xanthine oxidase, adenosine deaminase and xanthine oxidase were also demonstrated. A significant increase in the content of products of oxidative modification of proteins in plasma and erythrocytes was observed with aging. It was shown that key enzymes of antioxidant protection, i.e., superoxide dismutase and glutathione peroxidase, were reduced in blood plasma and erythrocytes with age. The results indicate that an imbalance in the antioxidant system occurs with age in blood plasma and erythrocytes, and this process contributes to the development of pathological conditions by increasing oxidative stress, which is especially pronounced with age. © 2015, Pleiades Publishing, Ltd.

Gorshkov O.,Donetsk National Medical University
AIP Conference Proceedings | Year: 2013

We suggest an algorithm for the estimation of the largest Lyapunov exponent for one-dimensional domains. We evaluate the largest Lyapunov exponent for one-dimensional domains, which present the surrogate long-range correlated stochastic time series with Hurst exponent H=0.1, H=0.9, H=0.5. It has been established that for an anticorrelated time series with the Hurst exponent H=0.1, the largest Lyapunov exponent is positive. For a correlated time series with Hurst exponent H=0.9, the largest Lyapunov exponent is negative. Also for a classical random walk with Hurst exponent H=0.5, the largest Lyapunov exponent is close to zero. © 2013 AIP Publishing LLC.

Panova T.I.,Donetsk National Medical University
Neurophysiology | Year: 2013

An overview of the reports published within the recent period (2003-2012) and related to the roles of influences addressed to the cerebral reinforcement (reward) system and also to the roles of gene mutations in the development of alcoholism is presented. The specific role of the effects of alcohol and also of other addictive agents and stimuli on the cerebral dopaminergic neuronal system and importance of changes realized in this case in the structures related to the cerebral reward system, first of all in the nucl. accumbens and cerebral structures connected with this nucleus, are described. Receptor mechanisms of the effects of addictive agents (roles of different types of dopamine and GABA receptors) are analyzed. The similarity of the effects of different addictive compounds on the cerebral reinforcement system is emphasized. The first results of therapeutic using of deep brain stimulation for the treatment of alcoholism and prospects of such approaches have been mentioned. The importance of gene mutations in the formation of predisposition for alcoholism, in particular of mutations influencing the synthesis of alcohol dehydrogenases, aldehyde dehydrogenases, cell adhesion molecules, neurospecific protein kinase C, dopamine and noradrenalin receptors and transporters, and also of some regulatory proteins, is considered. © 2013 Springer Science+Business Media New York.

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