Dolphin PG Institute of Biomedical and Natural science

Dehradun, India

Dolphin PG Institute of Biomedical and Natural science

Dehradun, India
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Arora S.,University of Petroleum and Energy Studies | Kumar D.,Dolphin PG Institute of Biomedical and Natural science | Shiba,Dolphin PG Institute of Biomedical and Natural science
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2013

Phytochemical, antimicrobial, and antioxidant investigation on the leaves and flower of Ipomoea cairica was traced in present investigation. Leaves and flowers of the plants were extracted with Methanol. Methanol Extracts of both leaves and flower of Ipomoea cairica were tested quantitatively for phytoconsituents. Methanol extract for both leaves and flower showed the presence of phytoconstituent Alkaloids, carbohydrates, tannins, phenolic compounds, proteins and amino acid, terpinoids and sterols and saponins. These extracts were further tested for their antimicrobial activity against the bacterial strains of Eschirechia coli (22 mm & 11 mm), Klebsella pneumonia (11 mm & 10 mm), Salmonella typhi (13 mm & 11 mm), Bacillus subtilus (10 mm & 15 mm), Staphylococcus aurius (08 mm & 13 mm) and the fungal strains of Aspergillus niger (16 mm & 14 mm), Penicillum chrysogenum (20 mm & 18 mm), Sacchomyces cerevisiae (25 mm & 21 mm), Candida albicans(24 mm & 18 mm) of both methanol extract of leaves and flowers respectively. The results have shown that methanol extract of the leaves and flower of Ipomoea cairica showed very good activity against all the bacterial and fungus strains in comparison with chloramphenicol and ketoconazole. Methanol extracts of leaves and flowers showed remarkable antioxidant activity 82. 58% and 81. 44% at 500 μg/ml respectively.


Kumar V.,Uttaranchal University | Nanda M.,Dolphin PG Institute of Biomedical and Natural science | Verma M.,Uttaranchal University | Verma M.,Indian Institute of Technology Roorkee
Bioresource Technology | Year: 2017

In order to increase microalgal biomass productivity efficient cultivation and harvesting methods are needed against the available traditional methods. The present study focuses on the same by harvesting microalgae using agar gel. Agar medium containing bold's basal medium (BBM) undergoes a thermoreversible gel transition. As compared to the traditional protocols, this gel is used to cultivate microalgae without even affecting the total productivity. To develop the gel for microalgae cultivation, agar was boiled in BBM. Then the agar was cooled to 35 °C and microalgae culture was added to it. After seeding the microalgae the temperature of the agar was further decreased by 10 °C to induce gelation. Instead of isolated cells microalgae were grown in clusters within the agar gel. Microalgal clusters gravimetrically settle at the bottom within 2 h. In this method agar can be reused. © 2017 Elsevier Ltd


Kumar R.,Hemwati Nandan Bahuguna Garhwal University | Kumar A.,Dolphin PG Institute of Biomedical and Natural science | Singh D.,Hemwati Nandan Bahuguna Garhwal University
New Biotechnology | Year: 2011

In an effort to optimize the medium components, the statistical methodology was applied to achieve the optimum lipase production under shake flask conditions. The study was conducted in three steps on newly isolated Bacillus pumilus RK 31. In the first step, 12 different variables viz., Glucose, Olive oil, Yeast extract, Peptone, Tween 80, KH2PO4, MgSO4, NaNO3, CaCl2, Temperature, pH and Inoculum size were used to identify the most significant variables affecting lipase production using Plackett-Burman statistical design. Variance analysis showed that Olive oil, Tween 80 and KH2PO4 played significant role in lipase production. In the second step, the values of the above-identified three variables were optimized by central composite design using three-level-three-factor approach. The optimum values of Olive oil, Tween 80 and KH2PO4 were found to be 10.0ml/l, 5.0ml/l and 8.0g/l, respectively. KH2PO4 was found to be responsible for maximum lipase production of 5.59IU/ml, experimental and 5.03IU/ml, predicted. In the third step, the optimum predicted values of the three factors and lipase production were verified by experimental approach. The amount of lipase produced in the designated medium was in agreement with that of predicted values by statistical method. © 2010 Elsevier B.V.


Arora S.,Dolphin PG Institute of Biomedical and Natural science | Vijay S.,Dolphin PG Institute of Biomedical and Natural science | Kumar D.,Dolphin PG Institute of Biomedical and Natural science
Journal of Chemical and Pharmaceutical Research | Year: 2011

Modern pharmacopoeia still contain at least 25% drugs derived from plants and many others which are synthetic analogues built on prototype compounds isolated from plants. The work is being pursued on the phytochemical, antimicrobial and antioxidant investigation on the leaves of Spilanthes acmella. Successive extraction of the crushed leaves of the plants has been done with various solvents and these extracts were tested quantitatively. These extracts were further tested for their antimicrobial activity against the bacterial strains of Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa, Micrococcus luteus, Klebsiella pneumonaie and against the fungal strains of Aspergillus niger, Penicillum chrysogenum, Rhizopus arrhigus, Rhizopus stolonifer. The results have shown that the ethyl acetate and methanol extracts can act as a standard drug against the becterial strain of Klebsiella pneumonaie and the water and ethyl acetate extract also showed very good activity against the fungal strains of Rhizopus stolonifer and Rhizopus arrhigus.


Saklani A.,Sardar Bhagwan Singh PG Institute of Biomedical science and Research | Parcha V.,Sardar Bhagwan Singh PG Institute of Biomedical science and Research | Dhulia I.,Sardar Bhagwan Singh PG Institute of Biomedical science and Research | Kumar D.,Dolphin PG Institute of Biomedical and Natural science
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2012

Diabetes mellitus (DM) is a heterogeneous metabolic disorder characterized by common feature of chronic hyperglycemia with disturbance of carbohydrate, fat and protein metabolism and often associated with various complications. Combinations of two or more plants in the treatment of severe diseases have been beneficial. The present study reports the combined effect of Coccinia indica (Wight & Arn) and Salvadora oleoides (Decne) leaf extract on blood glucose level and certain other biochemical parameters in alloxan induced diabetic rats. The Combined Methanolic Extract (CMEt) of the two plants at a dose level of 150 mg/Kg showed significant (p<0.01) reduction in blood glucose level of diabetic rats compared to that of standard drug Glipizide (5 mg/kg body weight). CMEt also showed significant (p<0.01) effect on lipid profile, ALS/AST activity, and serum creatinine and urea levels, there by exhibiting its overall significant antidiabetic potential.


Arora S.,Dolphin PG Institute of Biomedical and Natural science
Journal of Pharmaceutical Sciences and Research | Year: 2011

There is a clinical need for new treatment option as a result of continued increase in the expression of resistance among bacterial and fungal pathogens. A number of compounds currently in development show promise. However, in some cases, there is concern that resistance may develop quickly to new compounds that are based on existing antimicrobial agents. So it is therefore desirable to develop new potent antifungal, antibacterial drugs which produces minimum or no side effect and low cost. In continuation of this studies, some benzimidazole derivatives have been prepared and screened their antimicrobial activities with various strains. Various benzimidazole derivative of o-phenylene diamine, 4,5-dimethyl-1,2-phenylene diamine, 4-chloro-1,2-phenylenediamine, 3,4- diaminobenzophenone and S-methylated 3,4-diaminobenzophenone have been prepared by the reaction of 4-isothiocyanato-4-methyl pentan-2-one. All synthesized derivatives have been screened with the various bacterial and fungal strains viz. Escherichia coli, Bacillus pumilus, Micrococcus luteus, Bacillus cereus, Klebsiella pneumonaie, Aspergillus niger, Aspergillus flavus, Trichosporum flavurusclem and Microsporum gypseum. After the antimicrobial studies, it were found that the S-methylated 3,4-diamino benzophenone derivative (V) acts as a standard drug against bacterial strain Klebsiella pneumonaie and all tested fungal strains, because it showed more inhibition zone than the standard drug Amoxycillin and Ketoconazole respectively.


Kumar L.,Dolphin PG Institute of Biomedical and Natural Science | Kumar L.,Sardar Bhagwan Singh PG Institute of Biomedical science and Research | Singh B.,Sardar Bhagwan Singh PG Institute of Biomedical science and Research | Adhikari D.K.,Indian Institute of Petroleum | And 2 more authors.
Process Biochemistry | Year: 2012

A newly isolated Rhodococcus sp. LKE-028 (MTCC 5562) from soil samples of Gangotri region of Uttarakhand Himalayan produced a thermostable esterase. The enzyme was purified to homogeneity with purification fold 62.8 and specific activity 861.2 U mg -1 proteins along with 26.7% recovery. Molecular mass of the purified enzyme was 38 kDa and values of K m and V max were 525 nM and 1666.7 U mg -1 proteins, respectively. The esterase was active over a broad range of temperature (40-100 °C) and pH (7.0-12.0). The esterase was most active at pH 11.0. The optimum temperature of enzyme activity was 70°C and the enzyme was completely stable after 3 h pre-incubation at 60°C. Metal ions like Ca 2+, Mg 2+ and Co 2+ stimulated enzyme activities. Purified esterase remarkably retained its activity with 10 M NaCl. Enzyme activity was slightly increased in presence of non-polar detergents (Tween 20, Tween 80 and Triton X 100), and compatible with oxidizing agents (H 2O 2) and reducing agents (β-mercaptoethanol). Activities of the enzyme was stimulated in presence of organic solvents like DMSO, benzene, toluene, methanol, ethyl alcohol, acetone, isoamyl alcohol after 10 days long incubation. The enzyme retained over 75% activity in presence of proteinase K. Besides hyperthermostability and halotolerancy the novelty of this enzyme is its resistance against protease. © 2012 Elsevier Ltd.


Vijay S.,Dolphin PG Institute of Biomedical and Natural science | Sati O.P.,Hemwati Nandan Bahuguna Garhwal University
Journal of Materials Science: Materials in Medicine | Year: 2011

Enteric copolymers of acrylic acid and methyl methacrylate (2.5:7.5 and 2:8) were prepared using tetrahydrofuran as solvent and AIBN as free radical initiator for colon targeting. FTIR and 1H NMR spectra of the copolymers showed absence of vinyl bond/protons present in the monomers suggesting successful polymerization. Flurbiprofen sodium microspheres (M1 and M2) made with the copolymers, by oil/oil solvent evaporation, were spherical, anionic (zeta potential -57.8 and -53.7 mV) and contained 5.47 and 5.89% drug. FTIR spectrum of microspheres showed peaks for aromatic C = C stretching and substituted benzene ring, indicating entrapment of flurbiprofen. PXRD revealed crystalline structure of flurbiprofen while copolymer and microspheres were amorphous. DSC thermograms showed a sharp melting endotherm of flurbiprofen sodium at 129.26°C against broad endotherms of copolymers and microspheres. The microspheres released 43 and 36% drug at pH 6.8 in 2 h and 99 and 96% at pH 7.4 in next 3-4 h.The microspheres did not adhere on gastric-mucosa at pH 1.2 but showed mucoadhesion time of 18 min and 9 min on intestinal mucosa at pH 6.8. Thus, the microspheres on oral administration, would release the drug in colon, suggesting the potential of the hemocompatible copolymers for pH dependent colon targeted drug delivery system. © 2010 Springer Science+Business Media, LLC.


Kumar V.,Uttaranchal University | Nanda M.,Dolphin PG Institute of Biomedical and Natural science
Energy Sources, Part A: Recovery, Utilization and Environmental Effects | Year: 2016

The declining reserves of fossil fuels and fossil fuel-related environmental issues, especially greenhouse gas (carbon dioxide, methane) emissions, have posed a great threat and challenge to the sustainability of the world economy, the global environment, and hence the quality of life of human beings. Biomass pyrolysis could help reduce both the world’s dependence on oil and CO2 production. These bio-oils have the potential to cut CO2 emission because they are made up of plants that use CO2 for growth. There is a need to integrate process operation and reactor design to improve the effectiveness of different processes used for biomass to produce multiple products using a combination of technologies. © 2016 Taylor & Francis Group, LLC.


Vijay S.,Dolphin PG Institute of Biomedical and Natural science | Sati O.P.,Hemwati Nandan Bahuguna Garhwal University
Journal of Materials Science: Materials in Medicine | Year: 2010

Acrylic acid (AA)-methyl methacrylate (MMA) based copolymers, in different molar ratios (3:7, 4:6, 5:5, 6:4, and 7:3) were synthesized using tetrahydrofuran as solvent and AIBN as free radical initiator. Increase in acrylic acid concentration promoted pH-dependent swelling of copolymer and copolymer AA:MMA (3:7) was selected due to minimum swelling. ATR/FTIR and 1H NMR spectra of the copolymer showed absence of vinyl bond/protons present in the monomers suggesting successful polymerization. The copolymer was hemocompatible. Flurbiprofen sodium microspheres made with the copolymer, by oil/oil solvent evaporation, were spherical, anionic (zeta potential -59.0 mV) and contained 4.53% drug. ATR spectrum of microspheres showed peaks for aromatic C=C stretching and substituted benzene ring, indicating entrapment of flurbiprofen. XRD analysis revealed crystalline structure of flurbiprofen while copolymer and microspheres were amorphous. DSC thermograms showed a sharp melting endotherm of flurbiprofen sodium at 129.26°C against broad endotherms of copolymer and microspheres having peaks at 82.24 and 86.59°C, respectively. The thermogram of microspheres did not show the melting peak of flurbiprofen. The microspheres exhibited no drug release at pH <6.8 and released 83.4 and 99% drug at pH 6.8 and 7.4 in 3 h. The microspheres did not adhere on gastric mucosa at pH 1.2 but showed mucoadhesion time of 28 min on intestinal mucosa at pH 6.8. Thus, the microspheres on oral administration, would release the drug in distal ileum, suggesting the potential of the hemocompatible copolymer for enteric coating for prolonged drug release. © 2010 Springer Science+Business Media, LLC.

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