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Anghel I.,Carol Davila University of Medicine and Pharmacy | Anghel I.,Doctor Anghel Medical Center | Grumezescu A.M.,Polytechnic University of Bucharest
Nanoscale Research Letters | Year: 2013

Prosthetic medical device-associated infections are responsible for significant morbidity and mortality rates. Novel improved materials and surfaces exhibiting inappropriate conditions for microbial development are urgently required in the medical environment. This study reveals the benefit of using natural Mentha piperita essential oil, combined with a 5 nm core/shell nanosystem-improved surface exhibiting anti-adherence and antibiofilm properties. This strategy reveals a dual role of the nano-oil system; on one hand, inhibiting bacterial adherence and, on the other hand, exhibiting bactericidal effect, the core/shell nanosystem is acting as a controlled releasing machine for the essential oil. Our results demonstrate that this dual nanobiosystem is very efficient also for inhibiting biofilm formation, being a good candidate for the design of novel material surfaces used for prosthetic devices. © 2013 Anghel and Grumezescu; licensee Springer.


Anghel A.G.,Carol Davila University of Medicine and Pharmacy | Anghel A.G.,Doctor Anghel Medical Center | Grumezescu A.M.,Polytechnic University of Bucharest | Chirea M.,University of Vigo | And 9 more authors.
Molecules | Year: 2014

Cinnamomum verum-functionalized Fe3O4 nanoparticles of 9.4 nm in size were laser transferred by matrix assisted pulsed laser evaporation (MAPLE) technique onto gastrostomy tubes (G- Tubes) for antibacterial activity evaluation toward Gram positive and Gram negative microbial colonization. X-ray diffraction analysis of the nanoparticle powder showed a polycrystalline magnetite structure, whereas infrared mapping confirmed the integrity of C. verum (CV) functional groups after the laser transfer. The specific topography of the deposited films involved a uniform thin coating together with several aggregates of bio-functionalized magnetite particles covering the G- Tubes. Cytotoxicity assays showed an increase of the G- Tube surface biocompatibility after Fe3O4@CV treatment, allowing a normal development of endothelial cells up to five days of incubation. Microbiological assays on nanoparticle-modified G- Tube surfaces have proved an improvement of anti- Adherent properties, significantly reducing both Gram negative and Gram positive bacteria colonization.


Anghel I.,Carol Davila University of Medicine and Pharmacy | Anghel I.,Doctor Anghel Medical Center | Holban A.M.,University of Bucharest | Andronescu E.,Polytechnic University of Bucharest | And 2 more authors.
Biointerphases | Year: 2013

The present study reports the fabrication and characterization of a novel nanostructured phyto-bioactive coated rayon/ polyester wound dressing (WD) surface refractory to Candida albicans adhesion, colonization and biofilm formation, based on functionalized magnetite nanoparticles and Anethum graveolens (AG) and Salvia officinalis (SO) essential oils (EOs). TEM, XRD, TGA, FT-IR were used for the characterization of the fabricated nanobiocoated WDs. Using magnetic nanoparticles for the stabilization and controlled release of EOs, the activity of natural volatile compounds is significantly enhanced and their effect is stable during time. For this reason the nanobiocoated surfaces exhibited a longer term anti-biofilm effect, maintained for at least 72 h. Besides their excellent anti- adherence properties, the proposed solutions exhibit the advantage of using vegetal natural compounds, which are less toxic and easily biodegradable in comparison with synthetic antifungal drugs, representing thus promising approaches for the development of successful ways to control and prevent fungal biofilms associated infections. © 2013 Anghel et al.; licensee Springer.


Anghel I.,Carol Davila University of Medicine and Pharmacy | Anghel I.,Doctor Anghel Medical Center | Holban A.M.,University of Bucharest | Grumezescu A.M.,Polytechnic University of Bucharest | And 6 more authors.
Nanoscale Research Letters | Year: 2012

This paper reports a newly fabricated nanophyto-modified wound dressing with microbicidal and anti-adherence properties. Nanofluid-based magnetite doped with eugenol or limonene was used to fabricate modified wound dressings. Nanostructure coated materials were characterized by TEM, XRD, and FT-IR. For the quantitative measurement of biofilm-embedded microbial cells, a culture-based method for viable cell count was used. The optimized textile dressing samples proved to be more resistant to staphylococcal and pseudomonal colonization and biofilm formation compared to the uncoated controls. The functionalized surfaces for wound dressing seems to be a very useful tool for the prevention of wound microbial contamination on viable tissues. © 2012 Anghel et al.; licensee Springer.


Anghel I.,Carol Davila University of Medicine and Pharmacy | Anghel I.,Doctor Anghel Medical Center | Grumezescu A.M.,Polytechnic University of Bucharest | Holban A.M.,University of Bucharest | And 4 more authors.
International Journal of Molecular Sciences | Year: 2013

Cutaneous wounds are often superinfected during the healing process and this leads to prolonged convalescence and discomfort. Usage of suitable wound dressings is very important for an appropriate wound care leading to a correct healing. The aim of this study was to demonstrate the influence of a nano-coated wound dressing (WD) on Candida albicans colonization rate and biofilm formation. The modified WD was achieved by submerging the dressing pieces into a nanofluid composed of functionalized magnetite nanoparticles and Satureja hortensis (SO) essential oil (EO). Chemical composition of the EO was established by GC-MS. The fabricated nanostructure was characterized by X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), Differential Thermal Analysis (DTA) and Fourier Transform-Infrared Spectroscopy (FT-IR). The analysis of the colonized surfaces using (Scanning Electron Microscopy) SEM revealed that C. albicans adherence and subsequent biofilm development are strongly inhibited on the surface of wound dressing fibers coated with the obtained nanofluid, comparing with regular uncoated materials. The results were also confirmed by the assay of the viable fungal cells embedded in the biofilm. Our data demonstrate that the obtained phytonanocoating improve the resistance of wound dressing surface to C. albicans colonization, which is often an etiological cause of local infections, impairing the appropriate wound healing. © 2013 by the authors; licensee MDPI, Basel, Switzerland.

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