General-Toshevo, Bulgaria
General-Toshevo, Bulgaria

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Ganeva G.,Bulgarian Academy of Science | Landjeva S.,Bulgarian Academy of Science | Belchev I.,Dobrudja Agricultural Institute | Koleva L.,University of Forestry
Cereal Research Communications | Year: 2014

Two segregating populations of doubled haploid (DH) wheat lines derived androgenetically from crosses 'Svilena' (susceptible) × A-38b-4-5-3-3 (highly resistant) and 'Svilena' × WWRN (moderately resistant to moderately susceptible) were characterized for resistance to common bunt. Disease incidence was evaluated after inoculation of seeds with a mixture of Tilletia foetida teliospores in two autumn sown field experiments. Two-gene model of inheritance of resistance in line A-38b-4-5-3-3 was suggested. The transgressive segregation in the latter population was indicative for a quantitative mode of inheritance. The DH lines were assessed for plant height, heading time and important yield components in a three-year field experiment without bunt infection. In both populations, transgressive segregation was observed for all agronomic characteristics. Although the disease incidence was positively correlated with most of the agronomic traits, genotypes combining bunt resistance with good yield potential were isolated from 'Svilena' × A-38b set of lines. These genotypes are valuable for breeding varieties designed for growing in low-input and organic farming systems. The two DH populations are suitable to be used for further studies on the genetic basis of bunt resistance.

Dimova D.,Institute of Agriculture | Mihova G.,Dobrudja Agricultural Institute | Vulcheva D.,Institute of Agriculture | Vulchev D.,Institute of Agriculture | Ivanova I.,Dobrudja Agricultural Institute
Bulgarian Journal of Agricultural Science | Year: 2010

The objective of the present study was to identify feed barley varieties for specific habitats, using polymorphism of grain storage proteins, as well as to construct their hordein formulas. Current biotypes were established in the studied cultivars Veslets, Aheloi 2 and Panagon as well as manifestation frequency. The electrophoretic mobility and molecular weights of the hordein components were found. The results obtained can serve as a basis for future studies on the use of biochemical markers in feed barley breeding and seed production.

Vassileva V.,Bulgarian Academy of Science | Demirevska K.,Bulgarian Academy of Science | Simova-Stoilova L.,Bulgarian Academy of Science | Petrova T.,Dobrudja Agricultural Institute | And 2 more authors.
Journal of Agronomy and Crop Science | Year: 2012

Recurrent drought periods of varying duration often cause extensive crop damage and affect wheat production in Southern Europe. This study compares biochemical and ultrastructural responses of four wheat (Triticum aestivum L.) cultivars to long-term field drought, and their contribution to final grain yield. Gel electrophoresis and immunoblotting analyses combined with transmission electron microscopy and grain yield evaluation were employed to assess drought susceptibility of the wheat cultivars. Two of them behaved as drought-tolerant, the other two presented as drought sensitive. Enhanced degradation of Rubisco large subunit (RLS), Rubisco small subunit (RSS) and Rubisco activase (RA) accompanied by an increased protease activity and reduced levels of heat shock proteins (HSP70) and dehydrins (DHNs) were associated with drought sensitivity. Drought tolerance coincided with relatively stable or increased HSP70 and DHN contents, and unchanged/higher levels of RLS, RSS and RA. Sensitive cultivars were more vulnerable to ultrastructural damages, showing obvious degradation of chloroplast membrane systems and depletion of leaf starch reserves. These drought responses affected yield potential, as tolerant cultivars gave higher yield under intense drought. Thus, our results provide additional insights into the complexity of plant drought responses, identifying multiple interacting traits that may serve as indirect selection criteria for wheat drought tolerance. © 2011 Blackwell Verlag GmbH.

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