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Kovalenko T.N.,Bogomoletz Institute of Physiology | Ushakova G.A.,Dnepropetrovsk National University named by Oles Gonchar | Osadchenko I.,Bogomoletz Institute of Physiology | Skibo G.G.,Bogomoletz Institute of Physiology | Pierzynowski S.G.,Lund University
Journal of Physiology and Pharmacology | Year: 2011

In this study we investigated the potential neuroprotective effect of 2-oxoglutarate (2-OG) on the hippocampus in the transient vessel occlusion ischemia model in the Mongolian gerbil. The morphological and biochemical studies were performed at 7 days after occlusion of carotid arteries. The acute reduction of NeuN-positive neurons in the CA1 pyramidal layer of the hippocampus was accompanied by increased staining intensity for GFAP-positive astrocytes, indicative of glial reaction. The neuron death in the CA1 area coincided with a strong 2.4 fold decrease in the membrane forms of neuronal cell adhesion molecules and elevated levels of astrocyte-specific proteins (soluble GFAP to 2,6 times; filament GFAP to 1,5 times; calcium-binding protein S-100b to 1,6 times). Treatment with 2-oxoglutarate (2.28 g/l drinking water) for between 7 and 21 days attenuated the neuronal death and reactive astrogliosis in this model of experimental ischemia by 20-50%. Our results suggest that 2-OG may prevent the disturbances of neural cells that usually take place during ischemic pathology.

Ushakova G.,Dnepropetrovsk National University Named by Oles Gonchar | Ushakova G.,Lund University | Fomenko O.,Dnepropetrovsk National University Named by Oles Gonchar | Fomenko O.,Lund University | And 2 more authors.
Annales Universitatis Mariae Curie-Sklodowska, Sectio DDD: Pharmacia | Year: 2010

The level of the non-invasive markers of hepatic fibrosis and astroglia damage under chronic hepatitis C (CHC) and 2-oxoglutarate treatment was investigated. The obtained data allow for the suggestion that S-100b level in the blood serum may be used as a marker of hepatic encephalopathy under CHC, the treatment with 2-oxoglutarate can prevent serious bram damage caused by ammonia effect.

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