DNA Fingerprinting Unit

Karnāl, India

DNA Fingerprinting Unit

Karnāl, India
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Alghafri R.,Dubai Police General Head Quarters | Zupanic Pajnic I.,University of Ljubljana | Zupanc T.,University of Ljubljana | Balazic J.,University of Ljubljana | Shrivastava P.,DNA Fingerprinting Unit
International Journal of Legal Medicine | Year: 2017

Rapidly mutating Y-chromosomal short tandem repeats (RM Y-STRs) were identified to improve differentiation of unrelated males and also to enable separating closely and distantly related males in human identity testing in forensic and other applications. RM-Yplex assay was developed as a single multiplex that is capable of simultaneously amplifying all currently known RM Y-STRs, and reproducibility and sensitivity testing were performed on reference samples. Additional analyses are necessary to test its suitability for analysing compromised forensic samples. For this purpose, we applied the RM-Yplex assay to approximately 70-year-old skeletons that were used as a model for poorly preserved, challenging forensic samples. We analysed 57 male skeletal remains (bones and teeth) from 55 skeletons excavated from the Second World War (WWII) mass graves in Slovenia. The RM-Yplex typing was successful in all 57 samples; there were 56% full profiles obtained, and in partial profiles, up to 7 locus drop-outs were observed and they appeared correlated with low DNA quantities and degradation of DNA obtained from WWII bone and tooth samples. The longest loci, DYS403S1b, DYS547, DYS627 and DYS526b, were the most often dropped-out RM Y-STRs. In spite of high frequency of drop-out events, the RM-Yplex typing was successful in all WWII samples, showing the possibility of successful amplification of at least half of the RM Y-STRs even from the most compromised samples analysed. © 2017 Springer-Verlag Berlin Heidelberg


Shrivastava A.,M.R.Research | Gupta M.K.,Jiwaji University | Singhal P.K.,M.R.Research | Shrivastava P.,DNA Fingerprinting Unit
Current Bioactive Compounds | Year: 2016

Background: Emergence and reemergence of infectious diseases are major problems in public health and global economics. Due to selective pressure and widespread use of antifungal drugs, there have been increasing reports of antifungal resistance. Thus, new therapeutic drugs and/or approaches are needed to improve the management of these diseases and to overcome these problems. Our Bacillus and Brevibacillus sp. possess non – peptide group of compounds that may be good candidates for use as antifungal agents. Methods: 7 strains of Bacillus and Brevibacillus sp. were isolated from different habitats of Jabalpur, central India and identified by 16S rRNA sequencing. These strains were tested for antifungal activity against the test pathogens, viz. Alternaria solani (MTCC 2101), Aspergillus niger (MTCC 404), Candida albicans (MTCC 1637), Curvularia lunata (MTCC 2030) and Fusarium solani (MTCC 3004). Aqueous extracts of each antifungal strain, showing antifungal activity, were tested for the presence of non – peptide compounds by GC-MS. Results: The 7 antifungal strains were identified as Bacillus anthracis B48, Bacillus cereus B46, Bacillus tequilensis B37 and B47, and Brevibacillus brevis B63, B86 and B87. Br. brevis B87 exhibited the maximum activity against all the pathogens and B. tequilensis B37 recorded the minimum. GC-MS profile of the extract of all the strains revealed a diverse array of non-peptide (prosthetic groups) compounds, but ketones and pyrroles were present in the highest concentrations. The non-peptide groups 3,6-Diisobutyl-2,5- piperazinedione and Hexahydropyrrolo[1,2-a]pyrazine-1,4-dione, present in higher concentration in Br. brevis B87, might be responsible for its maximum antifungal activity as compared to the other isolates. Conclusion: The ketones and pyrroles from our strains may have great antifungal potential and need to be exploited further in the management of fungal pathogens and diseases of humans, plants and animals. © 2016 Bentham Science Publishers.


Imam J.,DNA Fingerprinting Unit | Reyaz R.,DNA Fingerprinting Unit | Singh R.S.,DNA Fingerprinting Unit | Bapuly A.K.,DNA Fingerprinting Unit | Shrivastava P.,DNA Fingerprinting Unit
International Journal of Legal Medicine | Year: 2017

We analysed 15 autosomal STRs in 200 unrelated individuals (102 males and 98 females) and 17 Y-STRs in 102 unrelated males living in Jharkhand, India, to establish parameters of forensic interest. The examined autosomal STRs revealed high combined power of exclusion (CPE) and combined power of discrimination (CPD) as equal to 0.9999 and greater than 0.99999, respectively. The combined probability of match (CPm) and combined paternity index (CPI) for all 15 autosomal STR loci were found to be 5.15 × 10−18 and 6.83 × 105, respectively. A total of 97 unique haplotypes were obtained, of which 93 were observed only once. The haplotype diversity, discrimination capacity and matching probability for 17 Y-STR loci were 0.999, 0.951 and 1.09 × 10−2, respectively. The highest gene diversity values at the single copy locus DYS635 and multi-copy locus DYS385 a/b were 0.785 and 0.823, respectively. © 2017 Springer-Verlag Berlin Heidelberg


Shrivastava P.,DNA Fingerprinting Unit | Jain T.,DNA Fingerprinting Unit | Jain T.,Jiwaji University | Trivedi V.B.,DNA Fingerprinting Unit
International Journal of Legal Medicine | Year: 2016

An analysis of 15 autosomal short tandem repeat (STR) loci and 17 Y-STR loci was performed in 123 unrelated members of the Oraon tribal community of Central India. The combined power of discrimination (CPD) and combined power of exclusion (CPE) were greater than 0.99999 and 0.999989, respectively, for autosomal STRs. In addition, a total of 58 distinct Y-STR haplotypes were observed out of which 54 Y-STR haplotypes were observed only once. The haplotype diversity and discrimination capacity for 17 Y-STR loci was 0.997 and 0.906, respectively. © 2016 Springer-Verlag Berlin Heidelberg


Kataria R.S.,DNA Fingerprinting Unit | Tait Jr. R.G.,Iowa State University | Kumar D.,DNA Fingerprinting Unit | Ortega M.A.,University of Puerto Rico at Mayaguez | And 2 more authors.
Immunogenetics | Year: 2011

Toll-like receptor 4 (TLR4) is a receptor protein that binds pathogen ligands, which are mainly associated with Gram-negative bacteria. The objective of this study was to investigate the association of nucleotide polymorphisms in TLR4 with infectious bovine keratoconjunctivitis (IBK), or pinkeye, incidence in American Angus cattle. Animals with previously calculated breeding values for IBK susceptibility were used to identify two SNPs in TLR4; Int1 (A/G) in intron1 (-26 Ex2 position) and Ex3 (C/T) in exon3 (1,678 position). To investigate the possible role of these SNPs in IBK susceptibility, the disease incidence information was collected on 370 calves raised in Iowa at two time points-June or August (disease season) and October (at weaning) and genotyped using PCR-RFLP protocols. In statistical models including year, pasture management group, and SNP, the Int1 SNP had a significant effect on IBK infection rates both in-season (P∈<∈0.05) and at weaning (P∈<∈0.01), whereas the Ex3 SNP was not significant (P∈>∈0.79) at either time point. Furthermore, the Int1 SNP alone could account for 2.1% of phenotypic variation in IBK infection during the disease season and 3.0% of phenotypic variation in IBK infection at the time of weaning. These data indicate that there is a relationship between Int1 genotype and the rate of IBK infection in American Angus cattle. © 2010 Springer-Verlag.


Shrivastava P.,DNA Fingerprinting Unit | Jain T.,DNA Fingerprinting Unit | Trivedi V.B.,DNA Fingerprinting Unit
Annals of Human Biology | Year: 2016

Aim To estimate population parameters based on allele frequencies obtained for 15 polymorphic autosomal STR loci investigated in caste and tribal populations of central India (n = 419). Methods Multiplexed PCR amplifications of the 15 Autosomal STR Loci were performed and amplified products were genotyped using multi-capillary electrophoresis on an ABI 3100 genetic analyser. Parameters of population genetics and forensic interest based on the allele frequencies were calculated. Genetic affinity of the studied populations among themselves and with previously reported populations of India was also analysed using distance-based NJ tree and using PCA plot. Results All the 15 STR loci were highly informative and discriminating, with CPD of 0.999 99. Except for Brahmins and Rajput, all other studied populations were in Hardy–Weinberg equilibrium (HWE). The only tribe (Gond) population studied showed significant variation with the other four caste populations (Brahmin, Yadav, Rajput and Muslim) studied and formed a cluster with other previously reported tribal populations of India. Nei’s genetic distance based clustering pattern of the NJ tree and the PCA plot showed the same pattern of genetic relationship, i.e. caste and tribal populations formed a distinct cluster. Conclusions With respect to the distribution of alleles at each STR locus, the studied loci were found to be substantially polymorphic in all the studied populations, indicating good informativeness of all 15 STR markers. The population data generated in this study are useful for forensic, anthropological and demographic studies. © 2016 Informa UK Limited, trading as Taylor & Francis Group


Jain T.,DNA Fingerprinting Unit | Shrivastava P.,DNA Fingerprinting Unit | Trivedi V.B.,DNA Fingerprinting Unit
International Journal of Legal Medicine | Year: 2016

The aim of this study was to contribute new data on autosomal STR and Y-STR markers of the Majhi tribal community of Chhattisgarh, a state of central India. In order to improve available databases of forensic interest, we analyzed 15 autosomal STR markers in a population sample of 129 unrelated indigenous Majhi tribe and 23 Y-chromosomal STR markers in the 107 males of the sample. The combined power of discrimination (CPD) and combined power of exclusion (CPE) were greater than 0.999999 and 0.999998 respectively for autosomal STRs. In addition, a total of 64 distinct Y-STR haplotypes were observed out of which 31 Y-STR haplotypes were observed only once. The haplotypes diversity and discrimination capacity for 23 Y-STR loci was 0.989 and 0.589, respectively. The highest gene diversity values at the single copy locus DYS570 and the multi-copy locus DYS385 a/b were 0.805 and 0.952, respectively. Average gene diversity over loci was 0.652304 ± 0.325572. © 2016 Springer-Verlag Berlin Heidelberg


Shrivastava P.,DNA Fingerprinting Unit | Jain T.,DNA Fingerprinting Unit | Gupta U.,DNA Fingerprinting Unit | Trivedi V.B.,DNA Fingerprinting Unit
Legal Medicine | Year: 2015

The analysis of 12 X STR loci (DXS10103, DXS8378, DXS7132, DXS10134, DXS10074, DXS10101, DXS10135, DXS7423, DXS10146, DXS10079, HPRTB and DXS10148) belonging to four linkage group was done in 183 (100 males and 83 females) unrelated members of Bhil population. Heterozygosity among the studied 12 X STR loci showed a distribution of from 59.7% to 92.8%. No significant difference was recorded in the allele frequencies of males and females. The loci DXS10135 and DXS10101 were found to be most polymorphic. Haplotype diversity was found to be higher than 0.990 for all the four linkage groups. A total of 86, 69, 71 and 71 haplotypes were observed for linkage group I, II, III and IV, respectively. The results showed departure from Hardy-Weinberg equilibrium with respect to three loci DXS10079, DXS10135 and DXS10101. This is first report on these 12 X STR markers from India. All the loci in the Argus X 12 kit were fairly informative in the Bhil population and the population showed significant genetic variation with all the compared populations from other parts of the world. © 2014 Elsevier Ireland Ltd. All rights reserved.


PubMed | DNA Fingerprinting Unit
Type: Journal Article | Journal: International journal of legal medicine | Year: 2016

The aim of this study was to contribute new data on autosomal STR and Y-STR markers of the Majhi tribal community of Chhattisgarh, a state of central India. In order to improve available databases of forensic interest, we analyzed 15 autosomal STR markers in a population sample of 129 unrelated indigenous Majhi tribe and 23 Y-chromosomal STR markers in the 107 males of the sample. The combined power of discrimination (CPD) and combined power of exclusion (CPE) were greater than 0.999999 and 0.999998 respectively for autosomal STRs. In addition, a total of 64 distinct Y-STR haplotypes were observed out of which 31 Y-STR haplotypes were observed only once. The haplotypes diversity and discrimination capacity for 23 Y-STR loci was 0.989 and 0.589, respectively. The highest gene diversity values at the single copy locus DYS570 and the multi-copy locus DYS385 a/b were 0.805 and 0.952, respectively. Average gene diversity over loci was 0.6523040.325572.


PubMed | DNA Fingerprinting Unit
Type: Journal Article | Journal: International journal of legal medicine | Year: 2016

An analysis of 15 autosomal short tandem repeat (STR) loci and 17 Y-STR loci was performed in 123 unrelated members of the Oraon tribal community of Central India. The combined power of discrimination (CPD) and combined power of exclusion (CPE) were greater than 0.99999 and 0.999989, respectively, for autosomal STRs. In addition, a total of 58 distinct Y-STR haplotypes were observed out of which 54 Y-STR haplotypes were observed only once. The haplotype diversity and discrimination capacity for 17 Y-STR loci was 0.997 and 0.906, respectively.

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