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Barash M.,Forensic DNA and Biology Laboratory | Barash M.,Bond University | Reshef A.,Forensic DNA and Biology Laboratory | Voskoboinik L.,Forensic DNA and Biology Laboratory | And 3 more authors.
Journal of Forensic Sciences | Year: 2012

A sexual assault case resulted in a pregnancy, which was subsequently aborted. The alleged father of the fetus was unknown. Maternal and fetal types were obtained using the 11-locus AmpFℓSTR® SGM Plus® kit. The national DNA database was searched for the paternal obligatory alleles and detected two suspects who could not be excluded as father of the male fetus. Additional typing using the AmpFℓSTR® Minifiler™ kit, containing three additional autosomal loci, was not sufficient to exclude either suspect. Subsequent typing using the PowerPlex® 16, containing four additional loci, and Y-Filer™ kits resulted in excluding one suspect. Searching a database for paternal obligatory alleles can be fruitful, but is fraught with possible false positive results so that finding a match must be taken as only preliminary evidence. © 2012 American Academy of Forensic Sciences. Source


Oz C.,Forensic Biology Laboratory | Einot N.,Forensic Biology Laboratory | Dell'Ariccia-Carmon A.,DNA Database Laboratory | Berlyne S.,Forensic Biology Laboratory | Gafny R.,Forensic Biology Laboratory
Forensic Science International: Genetics | Year: 2013

In an effort to promote European cross-border cooperation in fighting crime and international terrorism the Treaty of Prüm was drafted and accepted within the European forensic community. This move led to the commercial development of new multiplex kits which introduced five new STR loci and promised better performance. Recently the Israel Police DNA Casework and Database laboratories adopted the PowerPlex® ESI kit for routine use in our laboratories. Presented in this paper are examples of three types of ambiguous results encountered during the implementation of the PowerPlex® ESI kit into routine work. These ambiguous products presented themselves in the form of (1) extreme variants outside of loci borders, (2) failure to amplify sister allele pairs or expression of null alleles and (3) episodes of loss of separation of adjacent microvariants primarily in mixture samples. The re-analysis of all these samples using the PowerPlex® ESX kit successfully and rapidly clarified all three categories of anomalies. Spotlighting such events to the forensic community, especially regarding the novel loci introduced in these next generation kits, can aid in raising the analyst's awareness to their future appearances and prevent possible erroneous conclusions. In addition, providing timely DNA results to investigating teams is of great importance and operational forensic laboratories do not have at their immediate disposal methods such as sequencing to elucidate such manifestations. We suggest that the complementary use of the PowerPlex® ESI and PowerPlex® ESX can provide a benefit for clarification purposes in routine casework. © 2013 Elsevier Ireland Ltd. Source


Zaken N.,DNA Database Laboratory | Motro U.,Hebrew University of Jerusalem | Berdugo R.,DNA Database Laboratory | Sapir L.E.,DNA Database Laboratory | Zamir A.,DNA Database Laboratory
Forensic Science International: Genetics | Year: 2013

This report demonstrates the limits of DNA identification when siblings are involved. The Israeli DNA database routinely amplifies suspects samples using the PowerPlex® ESI16 system (Promega). While uploading a series of suspects into the database software, we found an unusual high number of shared alleles between two suspects 31 out of 32 alleles. Verification of their demographic data identified them as brothers. After confirmation of their paternity affiliation using the AmpFlSTR®YFiler™ (Applied Biosystems), we used two other multiplexes kits to improve the differentiation rate. The PowerPlex® ESX17 System (Promega) added one locus, SE33, who exhibits four different alleles. The second kit, the AmpFlSTR®MiniFiler™ (Applied Biosystems) added three more loci. Only one allele difference was found. In order to increase the discrimination power between related and unrelated individuals, we recommend that the DNA laboratories consider using a larger multiplex typing kit in cases like the one informed here. © 2013 Elsevier Ireland Ltd. All rights reserved. Source


Raziel A.,DNA Database Laboratory | Oz C.,DNA Database Laboratory | Carmon A.D.,DNA Database Laboratory | Ilsar R.,Agentek 1987 Ltd. | Zamir A.,DNA Database Laboratory
Forensic Science International: Genetics | Year: 2012

During the course of routine database sample analysis in the Israel Police DNA database, an off-ladder D3S1358 allele, calculated to be >22.1, extending into the adjacent vWA locus was observed using Applied Biosystems SGM Plus™ kit. To verify the size of this D3S1358 long allele and to ensure it was not part of a trialle pattern in the neighboring locus, the sample was amplified using three of the European new generation STR multiplex kits: NGM TM (Applied Biosystem), Powerplex™ ESX and ESI (Promega). The results of these amplifications determined the variant to be a 22 allele. Subsequent sequencing confirmed this designation and revealed a nucleotide polymorphism. Ten additional SGM Plus™ profiled samples with D3S1358 alleles larger than 19, were re-analyzed using NGM TM and Powerplex™ ESX which also showed discordance in the calculated results between original SGM Plus™ designations and those obtained with the European new generation multiplexes. © 2011 Elsevier Ireland Ltd. All rights reserved. Source


Dell'Ariccia-Carmon A.,DNA Database Laboratory | Raziel A.,DNA Database Laboratory | Oz C.,DNA Casework Laboratory | Berdugo R.,DNA Database Laboratory | Zamir A.,DNA Database Laboratory
Journal of Forensic Sciences | Year: 2014

European forensic laboratories are replacing the STR multiplex kits with the new generation 16/17 STR kits. This study examines the influence of the new generation kits and the new Applied Biosystems 3500xL Genetic Analyzer on the designation of long D2S1338 and D19S433 off-ladder alleles. Different allele calls were obtained using the new NGM™ (Applied Biosystems) and PowerPlex® ESI™ (Promega) kits compared with AmpFℓSTR® SGM Plus™ kit (Applied Biosystems). Sequence analysis was used to determine accurate allele designation. The new multiplex kits and the 3500xL Genetic Analyzer improved accuracy of long allele designations. DNA databases worldwide include countless profiles obtained by previous kits. Discrepancies between the new and former technologies may cause failure to detect hits. Discordance is expected due to primer sequence differences between various kits. An additional discordance, occurring in long alleles, independent of primer sequence is reported in this study. © 2013 American Academy of Forensic Sciences. Source

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