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Duman M.,Diyarbakir Plant Protection Research Station | Guz N.,Ankara University | Sertkaya E.,Mustafa Kemal University
Biochemical Systematics and Ecology | Year: 2015

In this study, DNA barcoding was used in the identification of potential biological control agents of sunn pest adult parasitoid species, including Eliozeta helluo (F.), Phasia subcoleoptrata (L.), Ectophasia crassipennis (F.) and Elomyia lateralis (Meig). DNA analyses were assessed by sequencing cytochrome c oxidase subunit I (COI) gene. The obtained sequences were analyzed in terms of nucleotide composition, nucleotide pair frequency and haplotype diversity. Genetic divergence among haplotypes was estimated by constructing genetic distance matrix using DNA sequence variations, by Kimura 2-parameter model. Variable sites and average variations of the sequenced 603 base pair long DNA fragment were calculated. All COI barcodes were matched with reference sequences of expected species according to morphological identification. Neighbor-joining tree was drawn based on DNA barcodes and all the specimens clustered in agreement with their taxonomic classification at species level. The evolutionary history inferred using the UPGMA method indicated two distinct mitochondrial haplotype lineages. The genetic variation between sunn pest adult parasitoids will be useful in sunn pest management, regulatory and environmental applications. © 2015 Elsevier Ltd. Source


Turkolmez S.,Diyarbakir Plant Protection Research Station | Ciftci O.,Diyarbakir Plant Protection Research Station | Serce C.U.,Nide University | Dervis S.,Mustafa Kemal University
Canadian Journal of Plant Pathology | Year: 2015

Crown and root rot or decline is an increasing problem on sweet cherry in Mardin, Malatya, Elaziʇ and Diyarbakir provinces in eastern Turkey. A survey was carried out during 2012-2014 in 120 commercial cherry orchards for disease symptoms, which included poor growth with sparse off-colour foliage, reddish-brown discolouration of the crown and roots, wilting and dieback of the canopy, and in many cases, tree death. Fifty-eight Phytophthora isolates obtained during the course of this 3-year survey were examined and identified as Phytophthora palmivora on the basis of morphological characteristics. BLAST analysis of ITS region sequences of rDNA of five isolates revealed 99-100% identity with reference isolates of P. palmivora from GenBank and Phytophthora database. Isolates of P. palmivora were pathogenic on 12-month-old potted Mahaleb cherry (Prunus mahaleb L.) rootstock seedlings that were wound-inoculated on the roots and on the crown. Inoculated plants expressed similar symptoms to those observed in the field and almost all infected plants died within 3 months after transplanting. This study demonstrated that P. palmivora is the causal agent of cherry decline in eastern Turkey. To our knowledge, this is the first report of P. palmivora on this host plant. © 2015 © 2015 The Canadian Phytopathological Society. Source


Turkolmez S.,Diyarbakir Plant Protection Research Station | Ciftci O.,Diyarbakir Plant Protection Research Station | Canihos E.,Plant Protection Research Institute | Serce C.U.,Nigde University | Dervis S.,Mustafa Kemal University
Journal of Phytopathology | Year: 2015

Forty-nine Phytophthora isolates were obtained from roots and crown of apricot trees with symptoms of decline grown in commercial orchards in Malatya, Elaziğ and Diyarbakir provinces, Turkey, in 2011 and 2013. All of the recovered isolates were identified as Phytophthora palmivora on the basis of morphological characteristics. Blast analysis of ITS region sequences of rDNA of 5 isolates revealed 100% identity with a reference isolates of P. palmivora from GenBank. Isolates of P. palmivora were pathogenic on 12-month-old wild apricot rootstock 'Zerdali' plants that were wound inoculated on the roots and on the crown. This study demonstrated that P. palmivora is the cause of the crown and root rot found on apricot in Turkey. To our knowledge, this is the first report of P. palmivora on this host plant. © 2014 Blackwell Verlag GmbH. Source

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