Division Prime

Harker Heights, TX, United States

Division Prime

Harker Heights, TX, United States
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Nikolic D.,Senior Electrical Engineer | Tereapii T.,Division Prime | Lee W.Y.,Energy Specialist | Blanksby C.,Senior Renewable Energy Engineer
Energy Procedia | Year: 2016

In its approach to delivering a 100% renewable energy target across 12 islands by 2020, the Cook Islands presents a rare insight into how planning requirements of high penetration renewable island systems vary with scale. To support this ambitious plan the Asian Development Bank and the European Union fund the Cook Islands Renewable Energy Sector Project, which will construct up to six solar photovoltaic (PV) power plants with a total installed capacity of about 3 megawatts-peak coupled with battery to store electricity from solar energy. The first three islands have small, standardized, centralized solutions (solar PV coupled with battery with existing diesel backup). An order of magnitude larger, Aitutaki will be implemented as a centralized solution in two stages, allowing detailed data collection and capacity building. An order of magnitude larger again, Rarotonga requires progressive planning and implementation including distributed generation, advanced control and integration, and sophisticated commercial structures. © 2016 The Authors.

Genes related to key cellular pathways are frequently altered in B cell ALL and are associated with poor survival especially in high-risk (HR) subgroups. We examined gene copy number abnormalities (CNA) in 101 Indian HR B cell ALL patients and their correlation with clinicopathological features by multiplex ligation-dependent probe amplification. Overall, CNA were detected in 59 (59%) cases, with 26, 10 and 23% of cases harboring 1, 2 or +3 CNA. CNA were more prevalent in BCR-ABL1 (60%), pediatric (64%) and high WCC (WBC count) (63%) patients. Frequent genes deletions included CDNK2A/B (26%), IKZF1 (25%), PAX5 (14%), JAK2 (7%), BTG1 (6%), RB1 (5%), EBF1 (4%), ETV6 (4%), while PAR1 region genes were predominantly duplicated (20%). EBF1 deletions selectively associated with adults, IKZF1 deletions occurred frequently in high WCC and BCR-ABL1 cases, while PAR1 region gains significantly associated with MLL-AF4 cases. IKZF1 haploinsufficiency group was predominant, especially in adults (65%), high WCC (60%) patients and BCR-ABL1-negative (78%) patients. Most cases harbored multiple concurrent CNA, with IKZF1 concomitantly occurring with CDNK2A/B, PAX5 and BTG1, while JAK2 occurred with CDNK2A/B and PAX5. Mutually exclusive CNA included ETV6 and IKZF1/RB1, and EBF1 and JAK2. Our results corroborate with global reports, aggregating molecular markers in Indian HR B-ALL cases. Integration of CNA data from rapid methods like MLPA, onto background of existing gold-standard methods detecting significant chromosomal abnormalities, provides a comprehensive genetic profile in B-ALL. © 2017, Springer Science+Business Media New York.

Kopakka N.,Division Prime | Dalvi R.,Division Prime | Shetty D.L.,Division Prime | Das B.R.,Super Religare Laboratories | Mandava S.,Division Prime
International Journal of Gynecology and Obstetrics | Year: 2012

Objective: To assess the frequency of balanced autosomal translocations in patients with primary amenorrhea in an Indian population. Methods: Cytogenetic analysis was carried out among women referred from all parts of India for primary amenorrhea between 2002 and 2010. Clinical history and laboratory findings were taken into consideration to determine the diagnosis. G-banding with trypsin-Giemsa was performed to detect chromosome abnormalities. Results: There were 15 balanced autosomal translocations in 1100 patients. Two novel translocations were identified: 1 with mosaic pattern of X chromosome monosomy and male karyotype, together with balanced autosomal translocation of chromosomes 11 and 20 in both cell lines; and 1 with double Robertsonian translocation of chromosomes 14 and 21. Conclusion: Autosomal genes have a crucial role in reproductive development. More candidate genes need to be recognized for appropriate genetic counseling and clinical management. © 2011 International Federation of Gynecology and Obstetrics.

Tamura S.,Division Prime | Ohta K.,Division Prime | Kanai T.,Idemitsu Kosan Co.
Journal of Applied Polymer Science | Year: 2012

Biaxially oriented polypropylene (BOPP) film accounts for a large amount of polypropylene since it is well suited for food packaging films or industrial films, because of its high performance in terms of mechanical and optical properties. Recently machine speed has been increasing to obtain higher production rate and film thickness has become thinner to reduce the environmental load. To meet the demands, many researchers have been investigating stretchability of PP by connecting the stretching force at the yield point and crystalline structure. Many other studies have been conducted regarding the surface structure of BOPP. Although there were some cases that crater-like film surface roughness was formed on BOPP films, the formation mechanism of craters has not been clarified. In this report, new hypothesis of the crater-like film surface roughness formation mechanism is proposed by observing the transformation of crater from sheet to BOPP film and by investigating the relationship between the stress-strain curve and surface roughness change. © 2011 Wiley Periodicals, Inc.

Tamura S.,Division Prime | Tamura S.,Kanazawa University | Kuramoto I.,Division Prime | Kanai T.,Kanazawa University | Kanai T.,Idemitsu Kosan Co.
Polymer Engineering and Science | Year: 2012

Biaxially oriented polypropylene films are widely used for food packaging and industrial films. Recently, machine speed has been increasing in order to obtain higher production rate, and film thickness has become thinner to reduce the environmental load. The customers' requirements for better production ability and thinner films have been becoming more demanding, but their demands are not always met because of lack of film stretchability in the final product. To meet the demands, research on stretchability has been conducted with the goal of finding the optimum polypropylene molecular structure for developing a new product by analyzing stretching force-strain data using a table tenter, which was thought to be the parameter of stretchability. It was found that low crystallinity and wide molecular weight distribution were effective in improving the stretchability from the table tenter test. By running the test with a sequential and biaxially oriented stretching machine, it was verified that samples produced by the above designed polymer indicated good thickness uniformity, which was considered to be the barometer of stretchability. Furthermore, it was concluded that analyzing the stretching force-strain data obtained from a table tenter is a good method to predict machine speed and film thickness. © 2012 Society of Plastics Engineers.

Ahmad F.,SRL Ltd. | Dalvi R.,Division Prime | Mandava S.,Division Prime | Das B.R.,SRL Ltd.
Pathology, research and practice | Year: 2014

The strong association of diagnostic karyotype with clinical outcome has made cytogenetics one of the most valuable diagnostic and prognostic tools for acute myeloid leukemia (AML) till today. Complex chromosomal findings are reported to be seen in nearly 10-15% of adult AMLs and are generally associated with poor outcome. In the current report, we present the results of hematologic, immunophenotypic, cytogenetic, chromosomal microarray and molecular analyses of a 60-year-old female patient diagnosed with AML-M2. Cytogenetic analysis revealed complex chromosomal findings involving seven different chromosomes. However, cytogenetic analyses were not able to precisely unveil all karyotypic changes, hence chromosomal microarray was used for further characterization. The most interesting observation was identification of a t(7;12) (q11;q22) as part of this complex karyotype. To the best of our knowledge, this is the first report of identification of novel t(7;12) (q11;q22) as part of a complex karyotype in de novo AML-M2. Copyright © 2014 Elsevier GmbH. All rights reserved.

Kokate P.,Division Prime | Sawaimoon S.,Super Religare Laboratories Ltd. | Bhatia S.,Super Religare Laboratories Ltd. | Mandava S.,Division Prime
Genetic Testing and Molecular Biomarkers | Year: 2012

Aims: The HER-2/neu proto-oncogene is amplified in 15%-25% of breast cancers. In the current study, we evaluated HER-2/neu status of 396 cases of breast cancer by fluorescence in situ hybridization (FISH), and the results were correlated with immunohistochemistry (IHC) for HER-2/neu protein expression. Results: Overall, HER-2/neu amplification was observed in 38.4% of cases. Concordance between IHC and FISH was 90.4% considering only IHC score 0, 1 (negative), and 3 (positive). However, only 37.3% of the IHC score 2 (equivocal) cases showed HER-2/neu gene amplification. A majority of the discordant cases within the IHC negative (score 0 and 1) and IHC positive (score 3) were high-grade tumors. Polysomy 17 and monosomy 17 was seen in 7.3% of the total cases of each. Furthermore, a majority of FISH positive cases were noted in Intraductal Carcinoma grade III and cases with regional lymph nodal metastasis. Polysomy 17 was seen in 7.9% of the FISH positive cases and in 6.3% of the FISH negative cases. Monosomy 17, however was more preponderant in FISH negative cases. Conclusion: We believe that the FISH test should be considered as the gold standard in the estimation of the HER-2/neu status due to its increased sensitivity and better appreciation of aneusomy 17. © Copyright 2012, Mary Ann Liebert, Inc.

Ahmad F.,Super Religare Laboratories Ltd. | D'Souza W.,Super Religare Laboratories Ltd. | Mandava S.,Super Religare Laboratories Ltd. | Ranjan Das B.,Super Religare Laboratories Ltd. | Ranjan Das B.,Division Prime
Leukemia and Lymphoma | Year: 2011

Mutations of the WT1 gene have been reported as the most common abnormality after NPM1 and FLT3 gene mutations in acute myeloid leukemia (AML), while KIT mutations are predominantly found in core-binding factor (CBF) AMLs. We report for the first time the prevalence and distribution patterns of WT1 and KIT mutations in an Indian population of 150. Overall, 10 (6.7%) and four (2.7%) of the cases had WT1 and KIT mutations, respectively. Of the six mutations observed in exon 7, five were frameshift while the remaining one case showed a substitution mutation. In contrast to exon 7, no frameshift mutation was detected in exon 9, where all mutations were substitution mutations. Interestingly, we observed a novel mutation in exon 8 of the KIT gene resulting from the deletion of nine nucleotides and insertion of three nucleotides affecting the extracellular domain of the KIT receptor, while Asp816Tyr and Asp816His were commonly found in exon 17 of the KIT gene. The WT1 mutation was more prevalent in normal karyotype AML while KIT was associated with t(8;21). With respect to FLT3 and NPM1 mutations, WT1 was more predominant in FLT3 positive cases and less in NPM1 mutation cases, while no KIT mutation was found in FLT3/NPM1 positive cases. © 2011 Informa UK, Ltd.

Ahmad F.,Division Prime | Rajput S.,Division Prime | Mandava S.,Super Religare Laboratories Ltd. | Das B.R.,Division Prime
Genetic Testing and Molecular Biomarkers | Year: 2012

Background and Aim: Mutation in the CAAT/enhancer binding protein-α (CEBPA) gene has been reported as being one of the common genetic abnormalities in acute myeloid leukemia (AML) and is associated with a good clinical outcome. We intend to explore the prevalence of CEBPA mutations and evaluate the efficacy of fragment and sequencing analysis methods for CEBPA mutation detection in Indian AML patients. Materials and Methods: The coding region of the CEBPA gene was screened in 36 normal karyotype AML patients by fragment analysis and direct sequencing. Results: We identified five CEBPA sequence variations in three patient samples (8.3%) by direct sequencing analysis, of which three were novel mutations. These mutations were clustered mostly in the TAD1 and basic region leucine zipper region of the CEBPA protein. Six cases demonstrated a previously reported polymorphism. Two of the three positive cases showed double mutations, and one case had a single mutation. All five mutations were also detected by fragment analysis, indicating a sensitivity of 100% (5/5). No correlation with clinical parameters including age, sex, white blood cell count, hemoglobin, and platelet count between patients with and without mutation was observed. Interestingly, CEBPA mutations were significantly higher in patients with WT1 mutation, while no correlation with FLT3 and NPM1 was observed. Conclusion: We report for the first time the frequency of CEBPA mutation from an Indian patients (8.3%). The identification of novel CEBPA mutations added new insights into the genetic heterogeneity of AML. Our result suggests that the optimal approach for detecting CEBPA mutations in AML can be a combination of fragment analysis and direct sequencing. © Copyright 2012, Mary Ann Liebert, Inc.

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