Division for Animal Nutrition Animal Production Institute

Irene, South Africa

Division for Animal Nutrition Animal Production Institute

Irene, South Africa

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Nkosi B.D.,Division for Animal Nutrition Animal Production Institute | Nkosi B.D.,University of the Free State | Meeske R.,Outeniqua Research Farm | Langa T.,Division for Animal Nutrition Animal Production Institute | And 5 more authors.
Small Ruminant Research | Year: 2015

The present study aimed to evaluate effects of ensiling potato hash (PHW) with enzyme either alone or in combination with bacterial inoculants on the fermentation characteristics, aerobic stability and nutrient digestion using rams. The PHW was mixed with wheat-bran (B) at 80:20 (% fresh matter) and ensiled in 210L drums for 90 days. The PHWB mixture was treated as: control (untreated), viscozyme, an enzyme containing hemicellulase and pectinase from Aspergillus spp (denoted as Visco), Viscozyme+Lalsil fresh LB (Lactobacillus buchneri NCIMB 40788) (denoted as E+LAB1), and viscozyme+silosolve (containing strains of Lactobacillus plantarum (DSM 16568), Enterococcus faecium (DSM 22502) and Lactobacillus buchneri (DSM 22501) (denoted as E+LAB2). After 90 days of ensiling, PHWB silage samples were collected per treatment and analysed for fermentation characteristics and nutritive values. The PHWB silage was then mixed with ground alfalfa hay at 68:32 (% DM basis) and fed ad libitum to 24 (6 rams/treatment) matured South African Dorper rams (39.7±2.21kg live weight) in a digestibility study. The fibre fractions (aNDF and ADF) and ADL content of PHWB silage were significantly reduced (P<0.05) in the treated silages compared to the control (306g aNDF/kg DM and 124g ADF/kg DM) respectively. Lactic acid content was lowest (58g LA/kg DM; P<0.05) in the control compared to the treated silages. The E+Lab1 treated PHWB silage had lowest (P<0.05) content of CP (<142g CP/kg DM) and significantly greater (P<0.05) content of ammonia-N (>5.3 NH3-N g/kg TN) compared to other treatments. Acetic acid content was lowest (3.4g AA/kg DM; P<0.05) in the Visco treated silage compared to other treatments. This resulted in greater (P<0.05) CO2 production (41g CO2/kg DM) in the Visco treated silage compared to other treatments when exposed to air. However, the addition lactic acid bacteria (LAB) to viscozyme (E+LAB1 and E+LAB2) increased (P<0.05) the contents of LA and AA and improved silage aerobic stability compared to other treatments. Greater (P<0.05) intake of DM (1887g/d) and lower (P<0.05) digestibility of DM were obtained in rams fed on the control silage diet compared to those fed the treated PHWB silage diets. The E+LAB1 and E+LAB2 treatments improved (P<0.05) nutrient digestion, nitrogen (N) retention and energy balance in rams compared to control treatment. © 2015 Elsevier B.V.


Nkosi B.D.,Division for Animal Nutrition Animal Production Institute | Nkosi B.D.,University of the Free State | Meeske R.,Outeniqua Research Farm | Langa T.,Division for Animal Nutrition Animal Production Institute | And 4 more authors.
Small Ruminant Research | Year: 2016

The study evaluated the effects of ensiling forage soybean (Glycine max (L.) Merr.) with or without bacterial inoculants on the fermentation characteristics, aerobic stability and nutrient digestion using rams. Forage soybean (cv. Link LF 6466) that contains 268g dry matter (DM); 70g water-soluble carbohydrates (WSC)/kg DM; 169g crude protein (CP)/kg DM and pH of 7.04 was harvested at R6 stage, chopped to 15mm length and ensiled in 210L drums for 90 days. The forage was treated as follows: (i) control (2L water/t), (ii) Lalsil Dry LB, a heterofermentative LAB inoculant (denoted as LDLB), and (iii) Sil-All 4×4® W.S. a homofermentative LAB inoculant (denoted as Sil). After 90 days of ensiling, forage soybean silage (FSS) samples were collected per treatment and analysed for fermentation characteristics and nutritive values. The FSS was top-dressed with 500g concentrate and fed ad libitum to 18 (6 rams/treatment) matured Damara rams (48.2±2.51kg live weight) in a digestibility study. Sil inoculation improved (P<0.05) the DM content while reducing (P<0.05) the fibre fractions (aNDF and ADF) and ADL content of FSS compared to the other treatments. The LDLB treated FSS had a lower (P<0.05) DM content (258g DM/kg) and lactic acid content (LA) (38g LA/kg DM) while the acetic acid content (AA) (51g AA/kg DM) and ammonia-N (128.7 NH3-N/kg TN) were higher (P<0.05) compared to other treatments. The reduced (P<0.05) AA content (24.6g AA/kg DM) in the Sil treated FSS resulted in a reduced (P<0.05) aerobic stability of FSS as indicated by higher (P<0.05) CO2 production (27.9g CO2/kg DM) and reduced hours of aerobic stability of FSS compared to other treatments. The intake and digestibility of DM, OM and N were improved (P<0.05) in rams fed the Sil inoculated FSS compared to other treatments. In contrast, the digestibility of fibre fractions was reduced (P<0.05) in rams fed the Sil-treated FSS compared to other treatments. © 2015 Elsevier B.V.

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