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Bagriacik E.U.,Gazi University | Yaman M.,Gazi University | Haznedar R.,Gazi University | Sucak G.,Gazi University | Delibasi T.,Diskapi Education and Research Hospital
Journal of Endocrinology | Year: 2012

Bone marrow-derived mesenchymal stem cells are pluripotent cells that are capable of differentiating into a variety of cell types including neuronal cells, osteoblasts, chondrocytes, myocytes, and adipocytes. Despite recent advances in stem cell biology, neuroendocrine relations, particularly TSH interactions remain elusive. In this study, we investigated expression and biological consequence of TSH receptor (TSHR) interactions in mesenchymal stem cells of cultured human bone marrow. To the best of our knowledge, we demonstrated for the first time that human bone marrow-derived mesenchymal stem cells expressed a functional thyrotropin receptor thatwas capable of transducing signals through cAMP. We extended this study to explore possible pathways that could be associated directly or indirectly with the TSHR function in mesenchymal stem cells. Expression of 80 genes was studied by real-time PCRarray profiles.Our investigation indicated involvements of interactions between TSH and its receptor in novel regulatory pathways, which could be the important mediators of self-renewal, maintenance, development, and differentiation in bone marrow-derived mesenchymal stem cells. TSH enhanced differentiation to the chondrogenic cell lineage; however, further work is required to determine whether osteoblastic differentiation is also promoted. Our results presented in this study have opened an era of regulatory events associated with novel neuroendocrine interactions of hypothalamic-pituitary axis in mesenchymal stem cell biology and differentiation. © 2012 Society for Endocrinology. Source


Topal E.,Gazi University | Gucenmez O.A.,Gazi University | Harmanci K.,Diskapi Education and Research Hospital | Arga M.,Gulhane Military Medical Academy and Medical School | And 2 more authors.
Annals of Allergy, Asthma and Immunology | Year: 2014

Background Knowledge of factors that affect relapse will allow close monitoring of patients at risk, resulting in a decreased rate of readmission to the emergency department. Objective To determine risk factors associated with relapse within 7 days after treatment of asthma exacerbations in children. Methods This was a multicenter, prospective study of children with asthma attacks. Patients between the ages of 6 months and 17 years who met the criteria between June 2009 and September 2012 were considered. Results The study included 1177 patients (775 males [65.8%]) with a mean (SD) age of 70.72 (48.24) months. Of them, 199 (16.9%) had a relapse within 1 week after being discharged from the hospital. Factors independently associated with relapse identified by a logistic regression model for the 1,177 study visits were having taken a short-acting inhaled β2-agonist within 6 hours before admission (odds ratio [OR], 2.43; 95% confidence interval [CI], 1.728-3.426; P =.001), presence of retraction on physical examination (OR, 1.76; 95% CI, 1.123-2.774; P =.01), no prescription for high-dose inhaled steroids on release (OR, 2.02; 95% CI, 1.370-3.002; P <.001), and not being given a written instructional plan (OR, 1.55; 95% CI, 1.080-2.226; P =.02). Conclusion Whereas having taken short-acting β2-agonists within 6 hours before admission and the presence of retractions on physical examination increased the risk of relapse after treatment of the acute attack, being given high-dose inhaled steroids and a written instructional plan when being sent home reduced the risk. © 2014 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved. Source


Balci M.M.,Turkiye Yuksek Ihtisas Education and Research Hospital | Arslan U.,Samsun Education and Research Hospital | Kocaoglu I.,Turkiye Yuksek Ihtisas Education and Research Hospital | Kafes H.,Turkiye Yuksek Ihtisas Education and Research Hospital | And 2 more authors.
Acta Cardiologica | Year: 2013

Objective The objective of this study was to evaluate right ventricular systolic and diastolic functions with the use of conventional and tissue Doppler echocardiography in patients with slow coronary flow (SCF). Methods and results Patients who were detected to have SCF but otherwise normal epicardial coronary arteries between October 2010 and July 2011 were included in our study. The control group was selected from the patients with normal coronary arteries but no SCF. All patients underwent echocardiography to evaluate left and right cardiac functions with conventional methods and tissue Doppler imaging. The study consisted of 86 patients [59 (68.6%) males, mean age: 54 ± 10 years) with SCF. Sixty-six subjects [42 (63.6%) males, mean age: 55 ± 8 years] with normal coronary arteries without SCF constituted the control group. Tissue Doppler findings of left ventricular systolic and diastolic functions were significantly disturbed in the SCF group (myocardial performance index: 0.37 ± 0.02 vs. 0.28 ± 0.02, P < 0.001; E/A ratio: 0.9 ± 0.1 vs. 1.1 ± 0.1, P < 0.001). However, when the right ventricular functions were considered, no significant difference was observed between the 2 groups (myocardial performance index: 0.25 ± 0.10 vs. 0.25 ± 0.10, P = 0.9; E/A ratio: 0.50 ± 0.06 vs. 0.50 ± 0.08, P = 0.3; TAPSE: 26.2 ± 2.2 vs. 25.9 ± 2.2, P = 0.6). Conclusions Preserved right ventricular diastolic and systolic functions in contrast to the impaired left ventricular functions in patients with SCF was the main finding of our study. The exact mechanisms of this new finding should be investigated by further studies. Source


Purnak T.,Ankara Numune Education and Research Hospital | Olmez S.,Ankara Numune Education and Research Hospital | Torun S.,Yuksek Ihtisas Education and Research Hospital | Efe C.,Hacettepe University | And 6 more authors.
Clinics and Research in Hepatology and Gastroenterology | Year: 2013

Background and aims: Liver biopsy is the gold standard procedure for documenting liver damage in chronic hepatitis C (CHC), as for many other chronic liver diseases. Mean platelet volume (MPV) is a laboratory marker obtained from complete blood count (CBC) analysers in routine clinical practice. The goal of the present study was to evaluate whether MPV would be useful in predicting liver histologic severity in CHC. Patients and methods: A total of 59 patients with CHC and 25 control subjects were recruited into the present study. There were 26 men and 33 women in the CHC group and 12 men and 13 women in the control group. MPV was recorded at the time of admission. The clinical characteristics of CHC patients, including demographics, laboratory and liver biopsy findings, were reviewed. Results: A statistically significant increase in MPV values was observed in CHC patients (8.54±0.63 fL) compared to healthy controls (7.65±0.42 fL) (P<0.001). Moreover, MPV values were significantly higher among patients with advanced fibrosis as compared to those with mild fibrosis (8.99±0.57 fL vs8.19±0.50 fL P<0.001). Receiver operator characteristic (ROC) curve analysis suggested that the optimum cut-off point for MPV value in advanced fibrosis was 8.75 fL. (Sensitivity: 80.8%, specificity: 81.8%, positive predictive value [PPV] 77.8%, negative predictive value [NPV] 84.4%, accuracy 81.3%, AUC: 0.98 P<0.001). Conclusion: The current study showed that MPV is increased in CHC with advanced fibrosis. Calculation of MPV along with the use of other markers may give further information about liver fibrosis severity in CHC. © 2012 Elsevier Masson SAS. Source


Coban S.,Diskapi Education and Research Hospital | Idilman R.,Ankara University | Erden E.,Ankara University | Tuzun A.,Ankara University
Hepato-Gastroenterology | Year: 2011

Background/Aims: Serum gamma-glutamyl-transpeptidase (GGT) levels often increase in chronic hepatitis C. We aimed to identify whether GGT levels can predict sustained virological response (SVR) in patients with chronic hepatitis C and to investigate other potential predictive factors associated with SVR in patients with chronic hepatitis C treated with pegylated interferon and ribavirin at a single center. Methodology: We evaluated 112 consecutive patients with histologically proven chronic hepatitis C who were treated with pegylated interferon and ribavirin. As potential predictors of SVR to combination therapy, we analyzed age, gender, body mass index, pretreatment GGT and alanine transaminase levels, diabetes mellitus, receiving of anti-viral therapy before beginning combination therapy, viral load, and liver histology by use of a multivariate logistic regression model. Results: SVR to combination therapy was seen in 57.2% of the patients. Variables associated with lower rates of sustained response were liver steatosis (p=0.026), diabetes mellitus (p=0.027), receiving anti-viral therapy before beginning combination therapy (p=0.016), higher GGT levels before therapy (>50IU/mL, p<0.001), and advanced fibrosis stage (p=0.017). On logistic regression analysis, the only independent predictor of SVR was the GGT level before therapy (p=0.003). Conclusions: Low serum levels of GGT before treatment are associated with higher rates of SVR in patients with chronic hepatitis C treated with pegylated interferon and ribavirin. © H.G.E. Update Medical Publishing S.A. Source

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