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Damante C.A.,Discipline of Periodontics | Ducati P.,Discipline of Periodontics | Ferreira R.,Discipline of Periodontics | Salmeron S.,Discipline of Periodontics | And 5 more authors.
Photodiagnosis and Photodynamic Therapy | Year: 2015

Background: Antimicrobial photodynamic therapy (aPDT) in Dentistry has important effects as bacterial destruction in areas with periodontal disease. Some dyes applied in aPDT could present low pH and, consequently, result in tooth demineralization. This study evaluated demineralization produced by aPDT with toluidine blue O (TBO) at low pH and analyzed adhesion/proliferation of human gingival fibroblasts (HGF). Methods: In the 1st phase, bovine enamel and root dentin fragments received 2 treatments: PDT4 group (TBO-100μg/ml-pH 4-60s) plus laser (660nm, 45J/cm2, 1.08J, 30mW, 30s, spot 0.024cm2, 1.25W/cm2, sweeping, non-contact) and CA group (citric acid plus tetracycline-pH 1-180s). Surface hardness loss and tooth wear were statistically analyzed (Student's t test, ANOVA/Tukey, p <0.05). In the 2nd phase, human dentin fragments were divided in C (control group-scaling and root planing), PDT4 and CA. HGF (104, 5th passage) were cultured on these fragments for 24, 48 and 72h and counted in scanning electron microscopy photographs. Number of HGF was analyzed using repeated-measures ANOVA and Tukey (p <0.05). Results: Percentage of surface hardness loss was similar in dentin for PDT4 (71.5%) and CA (76.1%) (p >. 0.05) and higher in enamel for CA (68.0%) compared to PDT4 (34.1%) (p <. 0.05). In respect to wear, no difference was found between PDT4 (dentin: 12.58. μm, enamel: 12.19. μm respectively) and CA (dentin: 11.74. μm and enamel: 11.03. μm) (p >. 0.05). Number of HGF was higher after 72. h in CA group (2.66, p <. 0.05) compared to PDT4 (2.2) and C (1.33). Conclusion: PDT4 is not as aggressive as CA for enamel. However, dentin demineralized promoted by PDT4 does not stimulate HGF adhesion and proliferation as CA. © 2015 Elsevier B.V. Source

Sant'Ana A.C.P.,Discipline of Periodontics | De Rezende M.L.R.,Discipline of Periodontics | Greghi S.L.A.,Discipline of Periodontics | Damante C.A.,Discipline of Periodontics
Lasers in Medical Science | Year: 2014

The acceleration of bone regeneration by low-intensity laser irradiation may hold potential benefits in clinical therapy in orthopedics and dentistry. The purpose of this study is to compare the effects of light-emitting diode (LED) and laser on pre-osteoblast MC3T3 proliferation and differentiation. Cells were irradiated with red, infrared, and LED (3 and 5 J/cm2). Lasers had a power density of 1 W/cm2 and irradiation time of 2 and 5 s. LED had a power density of 60 mW/cm2 and irradiation time of 50 and 83 s. Control group did not receive irradiation. Cell growth was assessed by a colorimetric test (MTT) (24, 48, 72, and 96 h), and cell differentiation was evaluated by alkaline phosphatase (ALP) quantification after growth in osteogenic medium (72 and 96 h and 7 and 14 days). At 24 h, the cell growth was enhanced 3.6 times by LED (5 J/cm2), 6.8 times by red laser (3 J/cm2), and 10.1 times by red laser (5 J/cm2) in relation to control group (p < 0.05). At the other periods, there was no influence of irradiation on cell growth (p > 0.05). The production of ALP was not influenced by irradiation at any period of time (p > 0.05). Low-intensity laser and LED have similar effects on stimulation of cell growth, but no effect on cell differentiation. © 2012 Springer-Verlag London. Source

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