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Klosterneuburg, Austria

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DirectSens GmbH | Date: 2015-12-09

Chemicals used in industry, science and photography, well as in agriculture, horticulture and forestry. Scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signaling, checking (supervision), life-saving and teaching apparatus and instruments.


Patent
DirectSens GmbH | Date: 2015-06-04

The present invention relates to cellobiose dehydrogenases (CDH) having glucose oxidation activity at a pH of 7.4 or above, modifications to modify the pH dependency of the enzymes activity, uses for these CDHs, in particular electrode sensors and electrochemical cells.


The present invention relates to a modified cellobiose dehydrogenase (CDH) or its functional flavodehydrogenase domain having glucose oxidation activity and a reduced maltose oxidation activity as compared to the unmodified CDH or its functional flavodehydrogenase domain, nucleic acids encoding said enzyme or domain, electrodes with said enzyme or domain and methods of producing and using the same.


Felice A.K.G.,University of Natural Resources and Life Sciences, Vienna | Sygmund C.,DirectSens GmbH | Harreither W.,DirectSens GmbH | Kittl R.,University of Natural Resources and Life Sciences, Vienna | And 4 more authors.
Journal of Diabetes Science and Technology | Year: 2013

Objective: Electrochemical sensors for glucose monitoring employ diferent signal transduction strategies for electron transfer from the biorecognition element to the electrode surface. We present a biosensor that employs direct electron transfer and evaluate its response to various interfering substances known to affect glucose biosensors. Methods: The enzyme cellobiose dehydrogenase (CDH) was adsorbed on the surface of a carbon working electrode and covalently bound by cross linking. The response of CDH-modified electrodes to glucose and possible interfering compounds was measured by flow-injection analysis, linear sweep, and chronoamperometry. Results: Chronoamperometry showed initial swelling/wetting of the electrode. After stabilization, the signal was stable and a sensitivity of 0.21 μA mM-1 cm-2 was obtained. To investigate the influence of the interfering substances on the biorecognition element, the simplest possible sensor architecture was used. The biosensor showed little (<5% signal deviation) or no response to various reported electroactive or otherwise interfering substances. Conclusions: Direct electron transfer from the biorecognition element to the electrode is a new principle applied to glucose biosensors, which can be operated at a low polarization potential of -100 mV versus silver/silver chloride. The reduction of interferences by electrochemically active substances is an attractive feature of this promising technology for the development of continuous glucose biosensors. © Diabetes Technology Society. Source

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