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Chaduvula P.K.,Indian Agricultural Research Institute | Bhati J.,Indian Agricultural Research Institute | Rai A.,Indian Agricultural Research Institute | Gaikwad K.,National Research Center Plant Biotechnology | And 3 more authors.
Australian Journal of Crop Science | Year: 2015

Sorghum {Sorghum bicolor L. Moench) is the fifth major cereal crop in the world. Sorghum is moderately salt-tolerant crop and different genotypes shows different levels of tolerance. Therefore, the present work emphasizes on reconstruction, validation and annotation of salt stress genes of Sorghum. In order to identify genes responsible for salt stress in Sorghum, 6749 expressed sequence tags (ESTs) were mined from the web recourses. The downloaded ESTs were clustered and assembled into 823 contigs and biological functions were acquired to the most of the contigs through Gene Ontology (GO). 617 contigs were showed homology to Rice, Maize and Barley protein sequences. The remaining contigs were mapped on Sorghum genome and full length gene sequences were obtained by in-silico approach. Total 12 candidate genes were identified as salinity stress responsive genes and amplified in nine indigenous Sorghum genotypes. These candidate genes were further validated by means of molecular techniques in nine Sorghum cultivars. The study claims the possible involvement of the predicted genes in salt stress mechanism and may be useful in molecular breeding programme in Sorghum salinity research.

Hariprasanna K.,ICAR Directorate of Sorghum Research | Agte V.,Agharkar Research Institute | Patil J.V.,ICAR Directorate of Sorghum Research
Indian Journal of Genetics and Plant Breeding | Year: 2014

For formulating an appropriate breeding strategy, genetic control of grain iron (Fe) and zinc (Zn) in sorghum was studied in a half-diallel mating design with nine parents. Both additive and non-additive gene actions were important in the genetic control but dominant gene action was predominant in case of Fe. In case of grain Zn additive gene action was more important with a σ2gca/σ2sca ratio of 0.94. Predictability ratio was 0.14 for grain Fe and 0.65 for Zn. Significant correlation between mean parental performance and GCA effects (r = 0.86 for Zn, r = 0.67 for Fe) indicated that selection of genotypes with high mean Zn and/or Fe levels would be highly effective in selecting for high GCA.The results suggest the scope for heterosis breeding to improve grain Fe, while for improving grain Zn progeny selection in pedigree breeding will be effective. High Fe and Zn levels in both of the parental lines would be required to develop heterotic hybrids with high grain micronutrient contents. © 2014, Indian Society of Genetics and Plant Breeding. All rights reserved.

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