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Rajendranagar, India

Srivastava R.K.,Nd University Of Agriculture And Technology | Singh R.K.,Nd University Of Agriculture And Technology | Prasad R.D.,Directorate of Oilseed Research
National Academy Science Letters | Year: 2012

The use of bioagents is risk free and the best alternative to sustain plant protection. Rhizoctonia solani affect the seed and seedling health of chickpea by causing seed and seedling rot. It showed that all the Trichoderma isolates inhibited significantly the growth of the pathogen and it ranged from 41.4 (T. viridi 1) to 52.8 (T. viridi 2). Germination per cent ranged from 74 (T. viridi 4) to 90.3 (PDBTCH 10) was when treated with bio-agent where as in pathogen check it was only 32.6%. Maximum vigor index (2239.4) was recorded in T. harzianum (PDBCTH 10) where as in pathogen check it was 169.4 only. All bioagents treatment recorded significantly less disease incidence as compared to pathogen check. Minimum (13.3%) pre emergence mortality was recorded in seeds treated with T. viridi 2 Lowest post emergence mortality was recorded in T. viridi 1 (4.8%). In remaining bioagents treatments the post emergence mortality ranged from 16.7 to 27.9%. The results obtained clearly indicated that the efficacy of different isolates of same species also showed variation when tested against the same pathogen, therefore, it may be concluded that before application in the field the potentiality of every strain of the bioagents should be tested against target pathogen. Graphical Abstract: The results showed that all the Trichoderma isolates inhibited significantly the growth of the Rhizoctonia solani and also affects the seed germination. All bioagents treatment recorded significantly less disease incidence and post emergence mortality as compared to pathogen check. The results obtained clearly indicated that the efficacy of different isolates of same species also showed variation when tested against the same pathogen, therefore, it may be concluded that before application in the field the potentiality of every strain of the bioagents should be tested against target pathogen.[Figure not available: see fulltext.] © 2012 The National Academy of Sciences, India. Source


Kumar S.,CCS Haryana Agricultural University | Kumar S.,Central Institute of Post Harvest Engineering and Technology | Yadav P.,Directorate of Oilseed Research | Jain V.,CCS Haryana Agricultural University | Malhotra S.P.,CCS Haryana Agricultural University
Journal of Food Science and Technology | Year: 2014

Isozyme profile of antioxidative enzymes viz. superoxide dismutase (SOD), peroxidase (POX), catalase (CAT) and ascorbate peroxidase (APX) was studied during ripening and storage of two cultivars of ber fruit (Ziziphus mauritiana Lamk.) differing in their shelf-lives viz. Umran (shelf-life, 8-9 d) and Kaithali (shelf-life, 4-5 d). The profile revealed that Umran variety exhibited three bands each of SOD and POX while in Kaithali, these enzymes had two isoenzymes throughout ripening. CAT and APX, however, showed two isozymes each during ripening of both the varieties and the pattern remained the same at all the stages of ripening except at the initial stage i.e immature green stage where single CAT isozyme was visible. During storage, one extra band each of SOD and POX present only in Umran got disappeared at later stages of storage, whereas in Kaithali, the pattern remained unchanged. Also, there was no change in the pattern of CAT and APX isozymes during storage of both the varieties. One isozyme of CAT could be considered as ripening related while one isozyme each of SOD and POX could be related to higher shelf life of fruits. © 2011 Association of Food Scientists & Technologists (India). Source


Hannachi I.,National Institute of Agronomy of Tunisia | Hannachi I.,University of Turin | Hannachi I.,National Agronomic Institute of Tunisia | Hannachi I.,Directorate of Oilseed Research | And 21 more authors.
European Journal of Plant Pathology | Year: 2015

Fusarium oxysporum f. sp citri is a new soil-borne pathogen of mature citrus trees in Tunisia. ISSR and RAPD markers were used in this study to characterize strains of Fusarium oxysporum from citrus in Tunisia. Pathogenicity assays revealed that Fusarium oxysporum isolates caused from 32 to 95 % disease on seedlings of Citrus aurantium. Results showed that pathogenic isolates were classified into two groups on the basis of ISSR and RAPD fingerprints, suggesting a level of genetic specificity and diversity not previously identified on Fusarium oxysporum f. sp citri. Cluster analysis, principal coordinate analysis and population structure indicated that the different isolates appears to be related only to the host. Potential primers related to ISSR markers could be selected to characterize Tunisian strains of Fusarium oxysporum and determine genetic differences of the pathogen virulence. With this investigation we characterize Fusarium wilt on citrus trees by the mean of ISSR and RAPD markers. © 2015, Koninklijke Nederlandse Planteziektenkundige Vereniging. Source

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