Time filter

Source Type

Nagaraja Reddy R.,Directorate of Sorghum Research DSR | Nagaraja Reddy R.,Directorate of Medicinal and Aromatic Plants Research DMAPR | Madhusudhana R.,Directorate of Sorghum Research DSR | Murali Mohan S.,Directorate of Sorghum Research DSR | And 3 more authors.
Theoretical and Applied Genetics | Year: 2013

Sorghum, a cereal of economic importance ensures food and fodder security for millions of rural families in the semi-arid tropics. The objective of the present study was to identify and validate quantitative trait loci (QTL) for grain yield and other agronomic traits using replicated phenotypic data sets from three post-rainy dry sorghum crop seasons involving a mapping population with 245 F9 recombinant inbred lines derived from a cross of M35-1 × B35. A genetic linkage map was constructed with 237 markers consisting of 174 genomic, 60 genic and 3 morphological markers. The QTL analysis for 11 traits following composite interval mapping identified 91 QTL with 5-12 QTL for each trait. QTL detected in the population individually explained phenotypic variation between 2.5 and 30.3 % for a given trait and six major genomic regions with QTL effect on multiple traits were identified. Stable QTL across seasons were identified. Of the 60 genic markers mapped, 21 were found at QTL peak or tightly linked with QTL. A gene-based marker XnhsbSFCILP67 (Sb03g028240) on SBI-03, encoding indole-3-acetic acid-amido synthetase GH3.5, was found to be involved in QTL for seven traits. The QTL-linked markers identified for 11 agronomic traits may assist in fine mapping, map-based gene isolation and also for improving post-rainy sorghum through marker-assisted breeding. © 2013 Springer-Verlag Berlin Heidelberg. Source

Abirami K.,Indian Agricultural Research Institute | Abirami K.,Directorate of Medicinal and Aromatic Plants Research DMAPR | Singh R.,Indian Agricultural Research Institute | Baskaran V.,Indian Agricultural Research Institute
Indian Journal of Horticulture | Year: 2011

Selection of dwarf mango rootstocks through long range field trials is a laborious and time consuming process. Hence investigations were carried out to screen the mango genotypes for their vigour at seedling stage. Twelve polyembryonic and ten monoembryonic genotypes were screened for their vigour at nursery stage with different physiological parameters like stomatal density, phenolic contents, chlorophyll fractions, bark percentage and relative water content. Among the different parameters phenolic contents, bark percentage and chlorophyll fractions were found to be very useful in predicting vigour of mango rootstocks at nursery stage. Source

Nagaraja Reddy R.,Directorate of Sorghum Research DSR | Nagaraja Reddy R.,Directorate of Medicinal and Aromatic Plants Research DMAPR | Madhusudhana R.,Directorate of Sorghum Research DSR | Murali Mohan S.,Directorate of Sorghum Research DSR | And 3 more authors.
Molecular Breeding | Year: 2011

Molecular variation within known genes controlling specific functions provide candidate gene-based markers which are tightly linked with the trait of interest. Unigene-derived microsatellite markers, with their unique identity and positions, offer the advantage of unraveling variation in the expressed component of the genome. We characterized ≥12-bp-long microsatellite loci from 13,899 unique sequences of sorghum [Sorghum bicolor (L.) Moench] available in the NCBI unigene database for their abundance and possible use in sorghum breeding. Analysis of 12,464 unigenes (≥200-bp) using MISA software identified 14,082 simple sequence repeats (SSRs) in 7,370 unigenes, from which 1,519 unigene SSR markers were developed. The average frequency of SSR was 1 per1.6 kb and 1.0 per 1.1 unigene; hexamers followed by trimers were found in abundance, of which 33.3% AT-rich and CCG repeats were the most abundant. Of the 302 unigene SSRs tested, 60 (19.8%) were polymorphic between the two parents, M35-1 and B35 of a recombinant inbred line (RIL) mapping population. A mapping population consisting of 500 RILs was developed using the above two parents, and a subset of random 245 RILs was used for genotyping with polymorphic SSRs. We developed a linkage map containing 231 markers, of which 228 (174 genomic and 54 genic) were microsatellites and three were morphological markers. Markers were distributed over 21 linkage groups, and spanned a genetic distance of 1235.5 cM. This map includes 81 new SSRs, of which 35 (21 unigene and 14 genomic) were developed in the present study and 46 from other studies. The order of the SSR markers mapped in the present study was confirmed physically by BLAST search against the whole-genome shotgun sequence of sorghum. Many unigene sequences used for marker development in this study include genes coding for important regulatory proteins and functional proteins that are involved in stress-related metabolism. The unigene SSR markers used together with other SSR markers to construct the sorghum genetic map will have applications in studies on comparative mapping, functional diversity analysis and association mapping, and for quantitative trait loci detection for drought and other agronomically important traits in sorghum. © 2011 Springer Science+Business Media B.V. Source

Samantaray S.,Directorate of Medicinal and Aromatic Plants Research DMAPR | Geetha K.A.,Directorate of Medicinal and Aromatic Plants Research DMAPR | Hidayath K.P.,Directorate of Medicinal and Aromatic Plants Research DMAPR | Maiti S.,Directorate of Medicinal and Aromatic Plants Research DMAPR
Plant Biotechnology Reports | Year: 2010

Decamer RAPD primers were tested on dioeceious and hermaphrodite plants of Commiphora wightii to identify sex-specific molecular markers. Sixty different random decamer primers were screened out of which only three primers were found to be associated with sex expression. A ~1,280-bp fragment from the primer OPN06 was found to be present in all the female individuals. Another primer OPN 16 produced a unique ~400-bp amplification product in only hermaphrodite individuals. The third marker, OPA20 amplified a ~1,140-bp fragment from female and hermaphrodite DNAs, but failed to do so from the male plant DNAs. © Korean Society for Plant Biotechnology and Springer 2009. Source

Discover hidden collaborations