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Chatterjee A.,Central Institute of Medicinal and Aromatic Plants | Kumar S.,Directorate of Medicinal and Aromatic Plants Research | Chattopadhyay S.K.,Central Institute of Medicinal and Aromatic Plants
Biomedical Chromatography | Year: 2013

A simple, rapid, accurate and reproducible reverse-phase HPLC method has been developed for the identification and quantification of two alkaloids ephedrine and cryptolepine in different extracts of Sida species using photodiode array detection. Baseline separation of the two alkaloids was achieved on a Waters RP-18 X-terra column (250×4.6mm, 5μm) using a solvent system consisting of a mixture of water containing 0.1% Trifluoroacetic acid (TFA) and acetonitrile in a gradient elution mode with detection at 210 and 280nm for ephedrine and cryptolepine, respectively. The calibration curves were linear in a concentration range of 10-250μg/mL for both the alkaloids with correlation coefficient values >0.99. The limits of detection and quantification for ephedrine and cryptolepine were 5 and 10μg/mL and 2.5 and 5μg/mL, respectively. Relative standard deviation values for intra-day and inter-day precision were 1.22 and 1.04% for ephedrine and 1.71 and 2.06% for cryptolepine, respectively. Analytical recovery ranged from 92.46 to 103.95%. The developed HPLC method was applied to identify and quantify ephedrine and cryptolepine in different extracts of Sida species. © 2013 John Wiley & Sons, Ltd. Source


Sharma S.,CSIR - Central Electrochemical Research Institute | Chattopadhyay S.K.,CSIR - Central Electrochemical Research Institute | Singh M.,CSIR - Central Electrochemical Research Institute | Bawankule D.U.,CSIR - Central Electrochemical Research Institute | Kumar S.,Directorate of Medicinal and Aromatic Plants Research
Phytochemistry | Year: 2014

From the hexane and ethyl acetate extracts of the leaves of Sesbania aculeata, three novel chemical compounds were isolated and fully characterized as compound 1, (ceramide type); compound 2, (cerebroside type) and compound 3 as a triterpene acid 3-O-α-L-rhamnopyranoside along with nine known compounds (Tricontanol, Lauric acid, Palmitic acid, Heptadecanoyl-1-tridecanoic acid, β-sitosterol, stigmasterol, poriferasterol glucoside, ononitol and pinitol). The anti-inflammatory potential of all three compounds were evaluated using in vitro target based anti-inflammatory activity in LPS-stimulated macrophages. TNF-α is one of the mediators of various chronic inflammatory disorders and treatment of hexane leaf extract (HL), Ethyl acetate leaf extract (EAL) and compounds 1, 2 and 3 at a dose of 10 μg/mL showed significant (P < 0.001) inhibition of TNF-α, a pro-inflammatory cytokine. IL-6 was significantly (P < 0.05) inhibited by compound 1 and HL at a dose of 10 μg/mL as compared with vehicle treatment. In-vitro cell cytotoxicity study using MTT assay revealed that these compounds were non toxic to the normal cells. © 2014 Elsevier Ltd. All rights reserved. Source


Das M.,Directorate of Medicinal and Aromatic Plants Research
Indian Journal of Agricultural Sciences | Year: 2014

An investigation was carried out to study the impact of sowing date and row spacing on yield components and swelling factor of Plantago indica during 2009-10 & 2010-11 at Directorate of Medicinal and Aromatic Plants Research (DMAPR), Anand, Gujarat. Its an endeavour and a step forward to develop Good Agricultural Practices (GAP) in this newly introduced species. In this study, the sowing dates in five levels (30 October, 15 November, 30 November, 15 December and 30 December) and row spacings in six levels (50 × 15 cm, 60 × 15 cm, 65 × 15 cm, 70 × 15 cm, 75 × 15 cm and 80 × 15 cm) were evaluated. Results of experiment revealed that there were significant differences for sowing date and spacing effects on plant height, number of branch per plant, number of spikes per plant, number of grains per plant, biological yield and grain yield. The maximum grain yield belonged to sowing date 15 November and row spacing 50 cm. Maximum number of spikes per plant and grains per plant also belonged to 50 cm row spacing which resulted in increasing grain yield in this row spacing. The results revealed that sowing between 15-30 November is the best time for sowing in P. indica with suitable spacing either at 50 or 60 × 15 cm. However, seeds sown on 30 November showed significantly more swelling (12.0 cc/g) compared to those sown on other dates. Swelling factor varied from 9.0 to 12.0 cc/g. Hence, it can be concluded that the sowing date played an important role on the growth parameters and seed characteristics of Plantago indica. However, swelling factor in this species is a matter of concern which was not much influenced by these factors. Source


Samantaray S.,Directorate of Medicinal and Aromatic Plants Research | Dhagat U.M.,V.V. Nagar | Maiti S.,Directorate of Medicinal and Aromatic Plants Research
Plant Biotechnology | Year: 2010

Studies were undertaken to assess genetic relationships in seven species of Plantago and to evaluate the genetic variance within populations of P. ovata (Forsk.), P. indica (L.), P. arenaria (Waldst.), P. psyllium (Linn.), P. lanceolata (Linn.), P. serraria (Linn.) and P. coronopus (Linn.) by using random amplified polymorphic DNA (RAPD) markers. A total of 629 distinct DNA fragments ranging from 0.25 to >3.0 kb pwere amplified using 75 selected random decamer primers. The cluster analysis indicated that the seven species of Plantago formed three major clusters: the first one consisted of three species and the second and third one represented by two species only. A maximum similarity of 85% was observed in P. arenaria and P. psyllium. Plantago indica shared up to 5% similarity with P. ovata. The wide genetic distance was observed within populations of different Plantago species. Thus, these RAPD markers have the potential for conservation of identified clones and evaluation of genetic relatedness among the species. This is also helpful in breeding programme and provides a major input into conservation biology. © 2010 The Japanese Society for Plant Cell and Molecular Biology. Source


Samantaray S.,Indian Central Rice Research Institute | Phurailatpam A.,Directorate of Medicinal and Aromatic Plants Research | Bishoyi A.K.,Directorate of Medicinal and Aromatic Plants Research | Geetha K.A.,Directorate of Medicinal and Aromatic Plants Research | Maiti S.,Directorate of Medicinal and Aromatic Plants Research
Genetic Resources and Crop Evolution | Year: 2012

The Random Amplified Polymorphic DNA (RAPD) technique was used to amplify DNA segments, with the objective of finding markers linked to sex determination in male and female plants of Piper betle L. Two bulks of DNA were made drawing one each from male and female, by pooling an equal volume of DNA samples from each group of individual contributing to the bulk analysis. Fifty different random decamer primers were screened with the two bulks to identify markers associated with sex expression of which only four primers were found to be associated with sex expression. These four primers were then tested with individual plant DNA samples where sex-associated RAPD markers were identified. A ~1,400 and ~850 bp fragment from the primer OPA04 and OPN 02 respectively was found to be present in all the male individuals and absent in all the female plants. In another primer, a ~980 bp amplification product from the primer OPC 06 was present only in the female individuals. A common primer OPA 08 showed both male and female specific markers of 650 and 1,200 bp respectively. Thus, the three male- specific RAPD markers OPA04 1400, OPA08 650 and OPN02 850 and two female-specific markers OPA08 1200 and OPC06 980 can reliably differentiate the male and female plants of P. betle L. Ploidy comparison also showed the differences in male and female plants. © 2011 Springer Science+Business Media B.V. Source

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