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Eutropiichthys vacha were collected from two rivers namely Ganga (Patna, India) and Kosi (Madhepura, India) for population genetic and phylogenetic studies. Five OPA primers were used to generate the fragment patterns from the samples collected. Polymorphisms within and between populations were assayed using 5 random primers, and 45 loci were amplified ranging from 250 to 2,000 bp. The percentage of polymorphic loci was found 51.1% and 55.6% for Ganga and Kosi populations, respectively. Total genetic diversity was 0.2173, and the average coefficient of genetic differentiation was 0.0958. The highest level of genetic diversities within population as well as lower between populations suggested that lower differentiation rate between populations. Gene flow between Ganga and Kosi populations was 4.7. Nei's unbiased measure of genetic identity and genetic distances of two populations were found 0.9606 and 0.0402, respectively. Phylogenetic analysis by RAPD showed one common cluster between two wild populations (Patna and Kosi) though they are quite distant from each other but belongs to same drainage system. Source

Prusty A.K.,Project Directorate for Farming System Research PDFSR | Meena D.K.,Central Inland Fisheries Research Institute CIFRI | Mohapatra S.,Central Institute of Fisheries Education | Panikkar P.,Regional Center | And 3 more authors.
International Aquatic Research

As a new class of agricultural insecticides, synthetic pyrethroids are widely used to control insect pests. Synthetic pyrethroids have been shown to enter the aquatic environment from agricultural runoff or drift from aerial and ground-based spraying applications posing threat to fishes which are less tolerant to pesticides through direct exposure. These insecticides interfere with the sodium channel of the nervous system resulting in prolonged sodium tail current. Widespread application of these chemicals has warranted the attention of the ecologist to understand the impact of these chemicals on the aquatic environment. In this perspective, an updated account of toxicological evaluation of three type II synthetic pyrethroids, viz. deltamethrin, cypermethrin and fenvalerate in terms of their physico-chemical, metabolic, hematological, histological, behavioral and reproductive aspects with respect to the fishes has been presented which may be useful for policy makers, academics, environmental scientists and agricultural professionals needing ready access to this information. The aim of the present synoptic literature appraisal was to summarize the main effect of current use, type II synthetic pyrethroids (deltamethrin, cypermethrin and fenvalerate) on aquatic environment due to their persistence and accumulation. This article will focus on non-target organisms in inland fresh water environment with special reference to fin fishes and will critically evaluate the toxicity of these pyrethroids in terms of growth inhibition, metabolic disorders, neurotoxicity, reproductive failure, enzymatic dysfunction, haematological alterations, and tissue damages. The rationalized information in this milieu may be useful in ecological risk evaluation and human health management as fish serves as an important bio-indicator for aquatic systems health. © 2015, The Author(s). Source

Mallik S.K.,Directorate of Coldwater Fisheries Research DCFR | Shahi N.,Directorate of Coldwater Fisheries Research DCFR | Pandey N.N.,Directorate of Coldwater Fisheries Research DCFR | Haldar R.S.,Directorate of Coldwater Fisheries Research DCFR | Pande A.,Directorate of Coldwater Fisheries Research DCFR
Indian Journal of Animal Sciences

Indian snow trout (Schizothorax richardsonii) and golden mahseer (Tor putitora) from cage culture unit of Bhimtal Lake were examined for fish louse (Argulus) infestation from August 2008 to January 2009. The percentages of S. richardsonii infected were 51.21, whereas abundance and mean intensity of infestations were 1.05 and 2.06 respectively. Maximum prevalence (70.1%) was observed in September with least in December. Percentages of host infected in T. putitora were 25 with abundance and mean intensity of 0.34 and 1.36 respectively. One to two percentage of fish stock was fungal infected. Measurement of crustacean parasite illustrated carapace length (CL) comprised 75% of average total body length (5.4 mm). Prevalence was positively correlated (S. richardsonii: r = 0.88; and T. putitora: r = 0.91) with decrease in water temperature. The results of mean intensity of Argulus infestation indicated an initial stage of infection in both the species. So the present study draws immediate attentions of fish health management towards enhancement of fish production in coldwater aquatic resources of India. Source

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