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Destandau E.,CNRS Institute of Organic and Analytical Chemistry | Michel T.,University of Nice Sophia Antipolis | Toribio A.,Direction Recherche and Technology | Elfakir C.,CNRS Institute of Organic and Analytical Chemistry
Planta Medica | Year: 2015

Centrifugal partition chromatography is a liquid-liquid separation method well adapted for the fractionation or purification of natural compounds from plant extracts. However, following the preparative isolation, the fractions collected must be analysed by high-performance thin-layer chromatography or high-performance liquid chromatography to evaluate their composition and/or their purity. These additional steps are time-consuming and increase the risk of compound degradation. In order to get an instantaneous analysis of fraction content with structural information on the phytochemicals eluted, it is possible to hyphenate on-line centrifugal partition chromatography with mass spectrometry. Depending on the complexity of the extract, two different kinds of centrifugal partition chromatography-mass spectrometry coupling can be performed: centrifugal partition chromatography-mass spectrometry or centrifugal partition chromatography-high-performance liquid chromatography-mass spectrometry coupling. In the first case, one part of the centrifugal partition chromatography effluent is directly introduced in the mass spectrometry ionisation source to identify the eluted compounds, while in the second case, it is directed to a high-performance liquid chromatography-mass spectrometry system where compounds are first separated thanks to high-performance liquid chromatography and then identified using mass spectrometry. © Georg Thieme Verlag KG. Source


Genta-Jouve G.,University of Paris Descartes | Croue J.,CNRS Microbial Oceanography Laboratory | Weinberg L.,Direction Recherche and Technology | Cocandeau V.,Direction Recherche and Technology | And 5 more authors.
Phytochemistry Letters | Year: 2014

Isolation of minor compounds from natural extracts remains a complicated task and development of new protocols to facilitate the access to new structure is still needed. Application of a highly efficient 2D LC hyphenated to high resolution mass spectroscopy on the crude extract of the Mediterranean marine sponge Crambe crambe led to the identification of seven new compounds. The structure elucidation has been performed using 2D LC-MS/MS and NMR. The structure elucidation of these minor metabolites encouraged us to speculate on the metabolic pathway leading to this important family of sponge alkaloids. © 2014 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved. Source


Audoin C.,University of Nice Sophia Antipolis | Audoin C.,GREENSEA SAS | Holderith S.,Direction Recherche and Technology | Romari K.,GREENSEA SAS | And 2 more authors.
Journal of Planar Chromatography - Modern TLC | Year: 2014

Although the different practical steps of a high-performance thinlayer chromatography (HPTLC) experiment are now all automated, this technique is suffering from the lack of computational treatment of the data. A large number of qualitative and quantitative protocols have been developed using HPTLC for the study of plants extracts. While for quantitative analyses ultraviolet (UV) detection is mainly used, qualitative comparisons of different samples are often realized by direct visualization of the plates. In this context, we developed the first work-flow dedicated to the analysis of HPTLC data, including the steps commonly used in metabolomics analyses. This development resulted in the increase of features detected and, more importantly, in the reduction of the common diffusion drift observed during HPTLC plate development. The output is a peak list containing RF values, intensities, and areas that can be used for statistical analyses. © Akadémiai Kiadó, Budapest. Source

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