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Ramon-Hernandez J.,University of Habana | Cosme C.,Bioterio | Nodarse H.,Direccion de Investigaciones Clinicas | Cruz O.,Direccion de Produccion | And 14 more authors.
Biotecnologia Aplicada | Year: 2015

Interferons (IFNs) are a family of cytokines of well-defined biological properties and exert immunoregulatory an-tiviral effects and antiproliferative activity. Nevertheless, the clinical application of biopharmaceuticals based on IFNs has been limited by the relatively restrained bioavailability of these molecules in blood. In the last years, the pegylation technology (i.e., the conjugation of polyethyleneglycol molecules to peptides and proteins) has provided great advances for the development of formulations showing prolonged bioavailability and surpassing much of the limitations for the use of therapeutic polypeptides, such as IFNs. In this work, the IFN α 2b molecule was chemically modified by conjugating it to a two-branched PEG polymer of 40 kDa molecular weight. This was the first report on this molecular combination. Moreover, a technological process was designed for the conjugation, purification and formulation of the IFN α 2b-PEG molecule. The physico-chemical and biological characterization demonstrated that this molecule complied with the international parameters enforced by regulatory authorities for those biopharma-ceuticals to be used to treat human diseases such as hepatitis C. This molecule received the Sanitary Registration in Cuba in 2009. It was demonstrated that pegylation increased the mean half-time of IFN α 2b-PEG in blood either in preclinical or in clinical studies, in respect with the non-modified IFN molecule. Therefore, a technology platform was established to generate products pegylated with 40 kDa PEG. This research granted the 2014 Award of the Cuban National Academy of Sciences.

Rodriguez-Estrada E.,Instituto Nacional Of Enfermedades Respiratorias | Fernandez-Sanchez M.,Instituto Nacional Of Enfermedades Respiratorias | Munoz-Delgado J.,Instituto Nacional Of Psiquiatria Ramon Of La Fuente Muniz Inprf | Fresan-Orellana A.,Direccion de Investigaciones Clinicas | Reyes-Teran G.,Instituto Nacional Of Enfermedades Respiratorias
Biological Rhythm Research | Year: 2016

The Steven-Johnson syndrome (SJS) is characterized by a sudden onset of mucous membrane erosion (predominantly oral mucosa, lips, and conjunctivae) with widespread blistering of the skin involving up to 10% of the body surface area. It is almost always a drug-related reaction, although it can be caused by infections and immunizations. A 33-year-old man with recent diagnosis of HIV infection developed antiretroviral treatment (ART)-associated SJS. Physical activity and sleep parameters were recorded by wrist actigraphy in four different consecutive scenarios: baseline assessment, first ART regimen, hospitalization, and second ART regimen. Significant differences were observed in physical activity patterns between the four phases. No differences in sleep parameters were found. To our knowledge, this is the first study recording physical activity changes and sleep during a SJS reaction. © 2015 Taylor and Francis.

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