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Uczyski W.,Endocrinology | Wawrusiewicz-Kurylonek N.,Diabetology and Internal Medicine | Szypowska A.,Medical University of Warsaw | Iendo E.,Laboratory Diagnostics | And 3 more authors.
Experimental and Clinical Endocrinology and Diabetes | Year: 2012

There is increasing evidence that T-regulatory (Treg) cells could be used to prevent or cure autoimmune diseases including type 1 diabetes mellitus (T1DM). The aim of the present study was to verify the hypothesis that functional Treg cells can be generated from conventional T-cells separated from a small amount of peripheral blood of children with newly diagnosed T1DM (N=25). Methods: CD4 +CD25 - cells were cultured with Treg expander (CD3/CD28) and IL-2 for generating de novo Treg cells. The assessment of the expression of selected genes and proteins critical to Treg function and the proliferation assays were performed with the use of real-time RT-PCR and flow cytometry. Results: After a 4-week stimulation with Treg expander and IL-2, the percentage of T-regulatory cells was significantly higher compared to the cells treated with medium alone (with no difference between diabetic and control children). However, we found some disturbances in the gene expression at mRNA level for molecules crucial for T-reg function. The induced Tregs from diabetic and control children were fully functional as assessed in proliferation assays. In summary: despite some disturbances at mRNA level in the critical gene expression, the suppressive properties of induced Treg cells from diabetic and control children were effective. © Georg Thieme Verlag KG Stuttgart New York. Source


Kuzmicki M.,Medical University of Bialystok | Telejko B.,Diabetology and Internal Medicine | Lipinska D.,Diabetology and Internal Medicine | Pliszka J.,Medical University of Bialystok | And 6 more authors.
Gynecological Endocrinology | Year: 2014

Irisin is a novel myokine and adipokine which induces an increase in total body energy expenditure, improving insulin sensitivity and glucose tolerance in experimental animals. In the present study, serum irisin concentration was measured by an enzyme immunoassay in 130 women with gestational diabetes mellitus (GDM) and 140 BMI-matched patients with normal glucose tolerance (NGT). Median irisin level was significantly lower in the patients with GDM than in the NGT subjects (1703.3 [1354.8-2097.9ng/ml] versus 1873.8 [1519.8-2294.8ng/ml] , p=0.01); however, 3 months after childbirth its concentrations did not differ markedly between the two groups (1165.9 [872.1-1497.5] ng/ml versus 1139.0 [984.0-1376.7] ng/ml). In the whole group, irisin concentration correlated negatively with 2h glucose level (R=-0.14, p=0.03). In the women with NGT, irisin concentration correlated positively with ISOGTT (R=0.22, p=0.04) and the disposition index (DI120) (R=0.24, p=0.03), as well as negatively with 2h insulin level (R=-0.23, p=0.03) and HOMA-IR (R=-0.24, p=0.02). Multiple regression analysis revealed that 2h glucose and DI120 were the only variables significantly influencing serum irisin (β=0.158, p=0.03 and β=0.159, p=0.02, respectively). Our results suggest that serum irisin concentration increases markedly in pregnant women, but this increase seems to be significantly lower in patients with GDM. © 2014 Informa UK Ltd. All rights reserved. Source

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