Yu J.,Qingdao University |
Ye J.,Qingdao University |
Liu X.,Dezhou Peoples Hospital |
Han Y.,Qingdao University |
Wang C.,Qingdao University
Neurological Research | Year: 2011
Objectives: 4-N-trimethylammonium-3-hydroxybutyric acid (L-carnitine) is an endogenous mitochondrial membrane compound and some studies have reported that L-carnitine could effectively protect various cells against oxidative injury both in vitro and in vivo. In the present study, we used the human neuroblastoma SH-SY5Y cell line as an in vitro model and assessed the effect of L-carnitine on hydrogen peroxide (H 2O 2)-mediated oxidative stress and neurotoxicity. Methods: Cells in culture were treated with different concentrations of H 2O 2 alone or pretreated with Lcarnitine. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, transmission electron microscopy, agarose gel electrophoresis, biochemical methods, and Western blotting were employed in the present study. Results: Pretreatment with L-carnitine for 3 hours inhibited H2O2-induced cell viability loss, morphological changes, intracellular reactive oxygen species generation, and lipid peroxidation in a concentrationdependent manner. Endogenous anti-oxidant defense components including total anti-oxidative capacity, glutathione peroxidase, catalase, and superoxide dismutase were also promoted by L-carnitine. Meanwhile, H 2O 2-induced down-regulation of Bcl-2, up-regulation of Bax, and DNA damage and apoptosis were also inhibited in the presence of L-carnitine. Discussion: Taken together, these results suggest that L-carnitine may function as an anti-oxidant to inhibit H 2O 2-induced oxidative stress as well as regulation of Bcl-2 family and prevent the apoptotic death of neuronal cells, which might be beneficial for the treatment of oxidative stress in neurodegenerative diseases. © W. S. Maney & Son Ltd 2011.
Gao J.,Capital Medical University |
Ding X.,Capital Medical University |
Ke S.,Capital Medical University |
Xin Z.,Zhanhua Peoples Hospital |
And 3 more authors.
Journal of Clinical Gastroenterology | Year: 2014
OBJECTIVE: Radiofrequency (RF) ablation is an accepted nonsurgical treatment of hepatic hemangiomas, but with an appreciable complication rate. Our study aimed to assess the safety and efficacy of RF ablation, administered with either multitined expandable electrodes or with internally cooled cluster electrodes, in the treatment of large (≥10 cm diameter) hepatic hemangiomas. METHODS: We retrospectively reviewed our sequential experience of treating 43 large hepatic hemangiomas in 42 patients with RF ablation/multitined expandable electrodes or with RF ablation/internally cooled electrodes. Twenty-two hemangiomas in 21 patients were treated with expandable electrode (multitined electrode group), and 21 hemangiomas in 21 patients were treated with internally cooled cluster electrode (internally cooled electrode group). RESULTS: Among the 43 large hepatic hemangiomas, 27 subcapsular lesions were treated by a laparoscopic approach, and 16 lesions located in liver parenchyma were treated by a computed tomography-guided percutaneous approach. In the multitined electrode group, RF ablation treatment was performed in all 21 patients in 1 session. In the internally cooled electrode group, 18 patients were treated by RF ablation in 1 session, and 3 patients, with ≥14.0-cm single hemangioma, were treated with RF ablation in 2 sessions. Complete ablation was achieved in 81.8% (18/22) and 90.5% (19/21) in the multitined electrode group and the internally cooled electrode group, respectively (P>0.05). Ablation time for single hemangioma was shorter with the internally cooled electrode than with the multitined electrode (P<0.05). There were 79 complications related to ablation (2 major and 77 minor) in 31 patients. All 21 patients in the multitined electrode group experienced complications, compared with 10 of 21 patients (47.6%) in the internally cooled electrode group (P<0.05). Both of the 2 major complications occurred in the multitined electrode group. All the complications were treated successfully with conservative measures. CONCLUSIONS: RF ablation is a safe and effective treatment for large hepatic hemangiomas. Use of the internally cooled cluster electrodes and a more defensive treatment algorithm can reduce the complications. Copyright © 2013 by Lippincott Williams &Wilkins.
Ye J.,Qingdao University |
Han Y.,Qingdao University |
Chen X.,Qingdao University |
Xie J.,Qingdao University |
And 3 more authors.
Neurochemistry International | Year: 2014
Both oxidative stress and endoplasmic reticulum stress (ER stress) have been linked to pathogenesis of neurodegenerative diseases. Our previous study has shown that l-carnitine may function as an antioxidant to inhibit H2O2-induced oxidative stress in neuroblastoma SH-SY5Y cells. To further explore the neuroprotection of l-carnitine, here we study the effects of l-carnitine on the ER stress response in H2O2-induced SH-SY5Y cell injury. Our results showed that l-carnitine pretreatment could increase cell viability; inhibit apoptosis and ROS accumulation caused by H2O2 or tunicamycin (TM). l-carnitine suppress the endoplasmic reticulum dilation and activation of ER stress-associated proteins including glucose-regulated protein 78 (GRP78), CCAAT/enhancer-binding protein-homologous protein (CHOP), JNK, Bax and Bim induced by H2O2 or TM. In addition, H2O2-induced cell apoptosis and activation of ER stress can also be attenuated by antioxidant N-acetylcysteine (NAC), CHOP siRNA and the inhibitor of ER stress 4-phenylbutyric acid (4-PBA). Taken together, our results demonstrated that H2O2 could trigger both oxidative stress and ER stress in SH-SY5Y cells, and ER stress participated in SH-SY5Y apoptosis mediated by H2O2-induced oxidative stress. CHOP/Bim or JNK/Bim-dependent ER stress signaling pathways maybe related to the neuroprotective effects of l-carnitine against H2O2-induced apoptosis and oxidative injury. © 2014 Elsevier Ltd. All rights reserved.
Pan Q.,Shandong University of Traditional Chinese Medicine |
Zhou J.-L.,Dezhou Peoples Hospital |
Ding C.-Z.,Shandong University of Traditional Chinese Medicine
Chinese Journal of Cancer Prevention and Treatment | Year: 2016
OBJECTIVE: At present, radical nephrectomy is the gold standard for the treatment of renal cell carcinoma(RCC), but nephron sparing surgery has been widely carried out. Through the analysis of CT and contrast-enhanced CT of renal cell carcinoma, we aimed to explore the value of dynamic enhanced CT in different subtypes of RCC. METHODS: From April 10th 2013 to April 20th 2105, the triphasic dynamic enhanced CT documents of 136 patients with RCC were reviewed. Three subtypes of renal cell carcinoma were analyzed, including 110 cases of renal clear cell carcinoma, 10 cases of papillary RCC and 16 cases of chromophobe RCC. The presence or absence of cystic degeneration or calcification of pattern in different subtypes of RCC were analyzed. The CT values of each stage were measured and the enhancement index of the tumor cortical were calculated. The analysis of variance was used for comparison. RESULTS: The frequency of cystic change and necrosis was higher in clear cell carcinoma than in papillocarcinoma and chromophobe renal carcinoma (86.4%>60.0%>12.5%). Respective stage of CT values of renal clear cell carcinoma, papillary RCC and chromophobe RCC were listed as follows: flat scanning (35.6±5.2, 41.2±8.4, 36.2±4.6), cortical phase (118.3±18.4, 56.8±7.6, 65.5±7.5), parenchymal phase (106.5±17.6, 76.5±9.6, 86.2±8.2), excretory phase (85.6±12.8, 75.3±7.4, 82.2±6.8). The enhancement index of tumor in each stage were: cortical phase (1.23±0.26, 0.38±0.12, 0.46±0.14), parenchymal phase (0.94±0.22, 0.41±0.14, 0.58±0.18), excretory phase (0.68±0.21, 0.38±0.13, 0.61±0.14). Variance analysis of CT values in different subtypes of each stage (F values were 4.91, 116.01, 3.71, 23.77, all P<0.05). Variance analysis of enhancement index in different subtypes of each stage (F=115.42, 44.61, 10.81, all P<0.05). All the above indexes showed significant statistical difference among the three subtypes. CONCLUSIONS: Renal clear cell carcinoma, papillary RCC and chromophobe RCC have their own characteristics in CT features and the parameter of enhancement. These characteristics are closely related to their own pathological features. Enhanced feature is one of the most important parameters to identify three common subtypes of renal cancer and different CT parameters have great help in differential diagnosis. © 2016, Editorial Board of Chinese Journal of Cancer Prevention and Treatment. All right reserved.
Yang B.-K.,Peoples Hospital of Ningjin County |
Zhang X.-B.,Dezhou Peoples Hospital
Chinese Journal of Tissue Engineering Research | Year: 2015
BACKGROUND: Oyster shells and other shells of marine organisms can transfer into mineral salts, which is closer to the actual situation of human being. OBJECTIVE: To explore the effect of natural oyster shell nanocomposite material for bone defect repair. METHODS: Thirty adult big-eared white rabbits were randomly divided into two groups after establishment of bilateral radial bone defect models: experimental group and control group were given implantation of natural oyster shell nanocomposite material and injectable calcium sulfate bone graft, respectively. X-ray examination was done at weeks 2, 8, 12 after implantation to understand the osseointegration of bone grafts. Bilateral radial bone specimens were taken at 12 weeks to detect bending strength using biomechanical testing system and quantitatively analyze the osteogenesis using a color image analyzer. RESULTS AND CONCLUSION: At 2 weeks after implantation, the bone mineral density of bone grafts was lower in the two groups than the normal value, there was a clear boundary between the defect and graft, and no bone repair was found. At 8 weeks after implantation, the bone grafts were covered with thick soft tissues in the two groups, and the vascular component was reduced dramatically in the experimental group. At 12 weeks after implantation, the bone grafts were closely connected to the adjacent tissues in the two groups, with no boundary; the radius surface in the experimental group recovered to the normal level, and exhibited no difference from the normal tissues in aspects of morphology, texture and structure, but in the control group, there was still a clear projected boundary. The bending strength and osteogenic amount of the radius were significantly higher in the experimental group than the control group (P < 0. 05). These findings indicate that the natural oyster shell nanocomposite material for bone defect repair can have a good blending strength of the radius that can promote new bone formation. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.