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Bhattacharyya S.,Deys Medical Stores Manufacturing Ltd | Datta S.,University of Calcutta | Mallick B.,Lady Brabourne College | Dhar P.,University of Calcutta | Ghosh S.,University of Calcutta
Journal of Agricultural and Food Chemistry | Year: 2010

Three different cultivars of marigold flowers (Tagetes patula L.) (marigold orange, marigold yellow, and marigold red) were analyzed for the lutein ester contents, and the in vitro antioxidative activities of the flower extracts were compared. The total antioxidant capacity, reducing power, hydroxyl, DPPH, and ABTS•+ radical scavenging activities, iron chelation capacity, and inhibition of lipid peroxidation in a linoleic acid emulsion system were measured. Iron-mediated Fenton reaction was carried out to evaluate the protective effect of leutin against DNA damage. The marigold orange (MGO) variety contains the maximum amount of lutein. It also had the highest DPPH radical scavenging activity and ABTS radical scavenging activity, with an EC50 value of 0.344 mg/mL. It was also the most effective against lipid peroxidation and hydroxyl radical scavenging activities. The MGO extract has the maximum reducing power. Hepatic cell damage in iron-mediated Fenton reaction caused by free radicals was reduced by the marigold extracts. Marigold flowers of Indian variety can be effectively utilized to produce lutein ester, which can be used as a food supplement or as an accessible source of natural antioxidants. © 2010 American Chemical Society.

Bera A.K.,Deys Medical Stores Manufacturing Ltd | Pal B.,St Pauls C M College | Sen Gupta K.K.,Jadavpur University
International Journal of Chemical Kinetics | Year: 2012

Outer-sphere reduction of hexacyanoferrate(III) by some enolizable/nonenolizable aldehydes (viz., aliphatic, heterocyclic, and aromatic aldehydes) in alkaline medium has been studied spectrophotometrically at λ max = 420 nm. The reactions are first order each in [aldehyde] and [Fe(CN) 6 3-]. The rate increases with an increase in [OH -] in the oxidation of aliphatic and heterocyclic aldehydes, whereas it is independent of [OH -] in the reaction with aromatic aldehydes. The intervention of free radicals in the reaction mixture was carried out using both acrylonitrile and acrylamide scavenger in two different experiments. The kinetic results indicate that the oxidation of benzaldehyde in aqueous medium proceeds at a slower rate than the aliphatic aldehydes (other than formaldehyde) and furfural. The values of third-order rate constant (k 3) at 308 K in the oxidations of some aliphatic aldehydes and furfural follow the order (CH 3) 2CH- > CH 3CH 2- > CH 3- > C 4H 3O- > H-. The rate constants correlate with Taft's σ* value, the reaction constant being negative (-9.8). The pseudo-first-order rate constants in the oxidations of benzaldehyde and substituted benzaldehydes follow the order -NO 2 > -H > -Cl > -OCH 3. The Hammett plot is also linear with a ρ value (0.6488) for meta- and para-substituted benzaldehydes. The kinetic isotope effect for benzaldehyde (k H/k D = 1.93 at 303 K) was obtained. The rate-determining step is the outer-sphere formation of Fe(CN) 6 4- and free radicals, which is followed by the rapid oxidation of free radicals by Fe(CN) 6 3- to give products. The kinetic data and hence thermodynamic parameters have been used to distinguish enolizable and nonenolizable aldehydes. An attempt has also been made to correlate kinetic data with hydration equilibrium constants of some aliphatic aldehydes. Copyright © 2012 Wiley Periodicals, Inc.

Polley N.,se National Center For Basic Science | Saha S.,Jadavpur University | Adhikari A.,se National Center For Basic Science | Banerjee S.,Jadavpur University | And 3 more authors.
Nanomedicine | Year: 2015

Aim: Testing the potential of citrate-capped Mn3O4 nanoparticles (NPs) as a therapeutic agent for alternative rapid treatment of hyperbilirubinemia through direct removal of bilirubin (BR) from blood in mice. Materials & methods: NPs were synthesized and the mechanism of BR degradation in presence and absence of biological macromolecules were characterized in vitro. To test the in vivo BR degradation ability of NPs, CCl4-intoxicated mice were intraperitoneally injected with NPs. Results: We demonstrated ultrahigh efficacy of the NPs in symptomatic treatment of hyperbilirubinemia for rapid reduction of BR in mice compared with conventional medicine silymarin without any toxicological implications. Conclusion: These findings may pave the way for practical clinical use of the NPs as safe medication of hyperbilirubinemia in human subjects. © 2015 Future Medicine Ltd.

Darbar S.,Deys Medical Stores Manufacturing Ltd | Bhattacharya A.,Deys Medical Stores Manufacturing Ltd | Chattopadhyay S.,Deys Medical Stores Manufacturing Ltd
Asian Journal of Pharmaceutical and Clinical Research | Year: 2011

Comparative protective activity of polyherbal formulation Livina against a well-established drug Silymarin was assessed against Paracetamol induced hepatic injury in swiss albino mice. To clarify its effect on liver functions, eight-week-old male mice were injected with Paracetamol at the single dose of 200 mg kg-1 body weight intraperitonially once daily 28 days. The hepatoprotective effect of polyherbal formulation (Livina) was comparatively evaluated with Silmarin (25 mg kg-1) by measuring levels of serum markers such as alanine aminotransferase (ALT), aspertate aminotransferase (AST), alkaline phosphatase (ALP), γ-glutamyle transpeptidase (GGT), lipid peroxidase (MDA), and total and direct bilirubin levels in mice serum along with the histopathological studies to support the above mentioned parameters. The study found that the supplementation of Livina significantly (P<0.05, P<0.001) reduced the damaging effects on liver by Paracetamol. Histopathological changes (congestion of central vein, centrilobular necrosis and sinusoidal congestion) induced by Paracetamol were also reduced to a moderate extent in Livina treated mice. In both the cases the effectiveness of Livina were found to be almost parallel with Silymarin, indicating the herbal formulation to be almost as effective as the standard drug.

De Kumar A.,Deys Medical Stores Manufacturing Ltd | Bera A.K.,Deys Medical Stores Manufacturing Ltd | Ghatak S.,Deys Medical Stores Manufacturing Ltd | Chowdhury P.P.,Deys Medical Stores Manufacturing Ltd | And 2 more authors.
Journal of the Indian Chemical Society | Year: 2010

A simple, accurate, rapid, precise and economic reverse phase high performance liquid chromatographic method was developed for estimation of glimepiride in tablet dosage form using C18 (5 μ, 250 mm x 4.6 mm) column. The mobile phase was a mixture of phosphate buffer (pH = 7.0 ± 0.1) and acetonitrile in the ratio 40 : 60 v/v. The detection wavelength was 228 nm. The retention time was 1.9 min at a flow rate of 1 ml/min. The validation of the proposed method was carried out according to USP and ICH guidelines. The method was found to be linear (r2 = 0.9999), precise (%RSD ≤ 2), accurate (mean percentage recovery yields = 99.31) and selective. Due to its simplicity, accuracy and economic value, the proposed method can be used for routine quality control analysis of solid dosage forms of glimepiride.

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