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Ich'ŏn, South Korea

Han J.G.,Yonsei University | Han J.G.,Development of Daesang Corporation | Kulkarni A.,Sungkyunkwan University | Kulkarni A.,BIO FD and C Co. | And 9 more authors.
Science of Advanced Materials | Year: 2015

Marine green alga Chlorella vulgaris produces secondary metabolitesincluding functional nutrients and associated bioactive peptides to protect harmful and environmental stresses. These evidences suggest that Chlorella vulgaris extracts have several beneficial effects on skin homeostasis such as protection against UV radiation and reactive oxygen species (ROS). In this study, we newly identified cysteine-alanine-serine (CAS) tripeptide from Chlorella vulgaris using LC-MS/MS and NMR. We investigated whether the identified CAS has beneficial effect of the protection from ROS species on skin cells. CAS was treated on the human skin cell line and this resulted in increment of anti-oxidant genes. In addition, we identified the significant induction of procollagen C endopeptidase enhancer (PCOLCE) which is related to skin firming by CAS. Combined all together, these results suggest that microalgae-derived CAS tripeptidemight be a valuable biomaterial in modulating skin homeostasis through its antioxidant function. © 2015 by American Scientific Publisher. Source


Jeon J.Y.,Yonsei University | Jeon J.Y.,Development of Daesang Corporation | Kwon J.-S.,Development of Daesang Corporation | Kang S.T.,Development of Daesang Corporation | And 5 more authors.
Biotechnology Progress | Year: 2014

Lutein is a carotenoid with a purported role in protecting eyes from oxidative stress, particularly the high-energy photons of blue light. Statistical optimization was performed to growth media that supports a higher production of lutein by heterotrophically cultivated Chlorella vulgaris. The effect of media composition of C. vulgaris on lutein was examined using fractional factorial design (FFD) and central composite design (CCD). The results indicated that the presence of magnesium sulfate, EDTA-2Na, and trace metal solution significantly affected lutein production. The optimum concentrations for lutein production were found to be 0.34 g/L, 0.06 g/L, and 0.4 mL/L for MgSO4·7H2O, EDTA-2Na, and trace metal solution, respectively. These values were validated using a 5-L jar fermenter. Lutein concentration was increased by almost 80% (139.64 ± 12.88 mg/L to 252.75 ± 12.92 mg/L) after 4 days. Moreover, the lutein concentration was not reduced as the cultivation was scaled up to 25,000 L (260.55 ± 3.23 mg/L) and 240,000 L (263.13 ± 2.72 mg/L). These observations suggest C. vulgaris as a potential lutein source. © 2014 American Institute of Chemical Engineers. Source

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