Design Institute of Chengdu Military Region

Chengdu, China

Design Institute of Chengdu Military Region

Chengdu, China
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Song B.,Shanghai University | Zhang C.,Design Institute of Chengdu Military Region | Li G.,Shanghai University | Jin G.,Shanghai University | Liu C.,Shanghai University
Cellular Physiology and Biochemistry | Year: 2015

Background: Pancreatic ductal adenocarcinoma (PDAC) is an almost universally lethal disease. Deregulation or dysfunction of miRNAs contribute to cancer development. The role of miR-940 in PDAC remains unclear. Methods: The level of miR-940 in PDAC tissues and cell lines was measured by qRT-PCR. MiR-940 was over-expressed by miRNAs mimics transfection and reduced by miRNAs antisense oligonucleotides (ASO) transfection. Cell proliferation was analyzed by MTT assay and cell apoptosis was evaluated by FACS analysis. Targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. Myeloid differentiation primary response gene (88) (MyD88) protein level was assayed by immunohistochemistry and Western blot analysis. Results: Low miR-940 level and high MyD88 protein level in PDAC tissues were both correlated with low survival rate. Up-regulation of miR-940 inhibited PDAC cell lines growth while down-regulation induced cell growth. The 3' UTR of MyD88 was targeted by miR-940. Conclusions: Low level of miR-940 and high level of MyD88 in PDAC promoted PDAC cells growth which might be related to the low survival rate of PDAC patients. MiR-940 exerted its effect by targeting MyD88. © 2015 S. Karger AG, Basel.


Liu C.,Shanghai University | Zhang C.,Design Institute of Chengdu Military Region | Mitchel R.E.J.,Chalk River Laboratories | Cui J.,Shanghai University | And 4 more authors.
Cell Death and Disease | Year: 2013

Toll-like receptor-4 (TLR4) plays a critical role in innate and acquired immunity, but its role in radio-resistance is unknown. We used TLR4 knockout (KO,-/-) mice and gut commensal depletion methods, to test the influence of TLR4 and its' in vivo agonist on basal radio-resistance. We found that mice deficient in TLR4 were more susceptible to IR-induced mortality and morbidity. Mortality of TLR4-deficient mice after IR was associated with a severe and persistent bone marrow cell loss. Injection of lipopolysaccharide into normal mice, which is known to activate TLR4 in vivo, induced radio-resistance. Moreover, TLR4 in vivo ligands are required for basal radio-resistance. We found that exposure to radiation leads to significant endotoxemia that also confers endogenous protection from irradiation. The circulating endotoxins appear to originate from the gut, as sterilization of the gut with antibiotics lead to increased mortality from radiation. Further data indicated that Myd88, but not TRIF, may be the critical adaptor in TLR4-induced radio-resistance. Taken together, these data strongly suggest that TLR4 plays a critical role in basal radio-resistance. Our data suggest, it is important not to give antibiotics that may sterilize the gut before the whole body irradiation. Further, these data also suggest that management of gut flora through antibiotic or possibly probiotic therapy may alter the innate response to the total body irradiation. © 2013 Macmillan Publishers Limited. All rights reserved.


Zhu Y.,Shanghai University | Zhang C.,Design Institute of Chengdu Military Region | Gu C.,Shanghai Airport Authority | Li Q.,Shanghai University | Wu N.,Shanghai University
Cellular Physiology and Biochemistry | Year: 2016

Background/Aims: Non-small cell lung cancer (NSCLC) tissues overexpress USP14, which promotes tumor cell proliferation and is associated with shorter overall survival time. Methods: The expression of USP14 was assayed in many types of cancers. USP14 was up-and down-regulated using appropriate plasmid or lentiviral vector constructs and its effects on proliferation, cell colony number, and apoptosis rate were measured. A human NSCLC cell line was inoculated into nude mice and the survival rates were recorded. Results: We found USP14 amplification and overexpression in many different cancers. The overexpression of USP14 in USP14 low-expression cell lines promoted cell proliferation and migration, whereas USP14 downregulation suppressed tumor cell proliferation, decreased tumor cell colony number, increased apoptosis rate, and decreased cell migration and invasion. Conclusion: USP14 plays an oncogenic role in various types of cancer, and may thus represent a new cancer therapy target. © 2016 S. Karger AG, Basel.


PubMed | Design Institute of Chengdu Military Region, Nanjing University and Shanghai University
Type: | Journal: Scientific reports | Year: 2015

The role of Toll-like receptor-2 (TLR2) in radio-resistance remained largely unknown. TLR2 knockout (TLR2(-/-)) mice received radiation of 6.5 Gy, and then were studied. We found that radiation resulted in more severe mortality and morbidity rates in TLR2(-/-) mice. The cause of death in TLR2(-/-) mice may be severe and persistent bone marrow cell loss. Injection of the TLR2 agonist Pam3CSK4 into wild type (WT) mice induced radio-resistance. Myd88(-/-) mice were more susceptible to radiation. In conclusion, our data indicate that, similar to TLR4, TLR2 plays a critical role in radio-resistance.


Lei W.,Peoples Hospital Of Mianzhu | Liu Y.-E.,The PLA Second Artillery General Hospital | Zheng Y.,Third Peoples Hospital Of Chengdu | Qu L.,Design Institute of Chengdu Military Region
Medical Science Monitor | Year: 2015

Background: Oral squamous cell carcinoma (OSCC) is the sixth most common human malignancy worldwide. To develop new therapeutics requires elucidation of the underlying mechanism of OSCC pathogenesis. The role of miR-429 in OSCC remains unknown.Material/Methods: The level of miR-429 and ZEB1 in OSCC tissues and cell lines was measured by qRT-PCR. MiR-429 was downregulated by miRNAs antisense oligonucleotides (ASO) transfection and up-regulated by miRNAs mimics. Cell proliferation was analyzed by MTT assay. Cell apoptosis was revealed by FACS analysis. Targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay.Results: MiR-429 was down-regulated in OSCC tissues, and miR-429 overexpression inhibited OSCC cell lines growth and vice versa. Further, we found that miR-429 could inhibit zinc finger E-boxbinding homeobox 1 (ZEB1) expression, and that miR-429 and ZEB1 expression in OSCC tissues were negatively correlated.Conclusions: Our data demonstrate the tumor suppressor role of miR-429 in OSCC, and may provide a potential therapeutic target that warrants further investigation. © Med Sci Monit.


Cao G.,Design Institute of Chengdu Military Region | Yang X.,PLA University of Science and Technology | Li H.,PLA University of Science and Technology
Advances in Intelligent Systems and Computing | Year: 2014

The main objective of this work is the development of an intelligent monitoring system based on the Internet of Things (IoT). The core of the system is embedded in ARM microcontroller of LPC2478 together with technology of Internet of Things, which has so far applied in fields of emergency response, intelligent shopping, smart product management, and military field sensing and so on. This system can implement fault detection, processing, memory storage, and alarming of the operating status parameters, such as temperature of cooling system, oil temperature, oil pressure, engine rotation speed, oil temperature of torque converter as well as oil level of sump by means of acquiring the operating status data from various testing sensors and the like. Specifically, it can accomplish functions of special vehicle’s orientation and track using GPS/GPRS, directing the transport routes, output the special vehicle accumulative weights, transmitting operating status to monitor center, querying history data, remote maintenance support, and querying history data. The system can record various types of operating parameters and send them to monitoring center by network. It is therefore applicable to many different types of engineering vehicle. © Springer India 2014.


Jiang M.,PLA Logistical Engineering University | Guo X.,PLA Logistical Engineering University | Xiong X.,Design Institute of Chengdu Military Region
Shiyou Xuebao, Shiyou Jiagong/Acta Petrolei Sinica (Petroleum Processing Section) | Year: 2011

Aluminum complex grease is a kind of multifunction grease. It is suited to central lubrication system for which has good pump ability. It has been used extensively in metallurgy area. The effect of composition, condition and additives on the properties of aluminum complex grease has been discussed. The thickening ability of aluminum complex soap in paraffin oil is superior to that of aluminum complex soap in naphthene oil. Increasing viscosity of base oil can improve the properties of aluminum complex grease. The method of trioxyaluminum tri-isopropylate is superior to that of aluminum isopropylate, because the technology is stable and the property is good. By optimization of composition and condition, the high performance aluminum complex grease has been prepared. It possesses high dropping point, good colloidal stability, shear stability, oxidation stability, water resistance property, anti-corrosion and anti-wear property.


Su D.,PLA Logistical Engineering University | Su D.,Design Institute of Chengdu Military Region | Tu Y.,PLA Logistical Engineering University | Shen Y.,PLA Logistical Engineering University | And 2 more authors.
Yi Qi Yi Biao Xue Bao/Chinese Journal of Scientific Instrument | Year: 2014

When the frequency estimation of short signal is performed under low SNR condition, traditional signal fusion method has the disadvantages, such as poor noise immunity and narrow applicable range. A frequency estimation method based on cross information fusion for different frequency signals is proposed to improve the frequency estimation accuracy and extend the applicable scope. To eliminate the influence of different frequencies of multi-segment signals on spectral analysis, the focusing matrixes are constructed to focus the multi-segment different frequency signals to the same frequency and obtain the multi-segment same frequency signals. To utilize the signal information fully, the cross information fusion is carried out according to the complementary information of the multi-segment signals in time and frequency domains, and the cross information fusion spectra are obtained. To improve the performance of variance, the arithmetic mean value of the obtained cross information fusion spectra is calculated. Furthermore, the frequency estimation is achieved by searching for the spectral peak of the average cross information fusion spectrum. The simulation results show that the proposed method is superior in frequency estimation precision and applicable range in comparison with the existing methods, and the experiment results demonstrate the feasibility and effectiveness of the proposed method.


Wu N.,Design Institute of Chengdu Military Region | Zhang C.,Design Institute of Chengdu Military Region | Bai C.,Design Institute of Chengdu Military Region | Han Y.P.,Design Institute of Chengdu Military Region | Li Q.,Design Institute of Chengdu Military Region
Cellular Physiology and Biochemistry | Year: 2014

Background: Lung cancer is the leading cause of cancer-related mortality worldwide, with near 1.4 million deaths each year. NSCLC accounts for nearly 85% of all case of lung cancer. MiRNAs play important roles in regulation of gene expression at the post-transcriptional level. MiRNAs profiles may predict prognosis and disease recurrence in early-stage NSCLC. Our previous study proved that over-expression of ubiquitin specific peptidase 14 (USP14), a deubiquitinating enzyme, was associated with favorable prognosis in NSCLC patients and promoted tumor cells proliferation. Here, we tried to identify which miRNAs targeted USP14, and the roles of these miRNAs in NSCLC. Methods: MiR-4782-3p and its potential targeted genes were identified by bioinformatics algorithm. Dual luciferase reporter assay system was used to analyze the interaction between miR-4782-3p and targeted genes. Cell proliferation was assayed by MTT and BdU assay. MiRNAs and mRNA expression were assayed by qRT-PCR. USP14 protein level was assayed by Western blot. The role of miR-4782-3p in patients survival was revealed by Kaplan-Meier plot of overall survival. Results: Up-expression of miR-4782-3p in NSCLC cells decreased the USP14 expression. Down-expression of miR-4782-3p increased USP14 expression. In NSCLC specimen, Negative correlation between USP14 mRNA level and miR-4782-3p level was identified. Higher miR-4782-3p expression is associated with longer survival. USP14, ZEB2, XIAP overexpression reversed the inhibitory effect of miR-4782-3p. Conclusions: High expression of miR-4782-3p was associated with favorable prognosis in NSCLC patients. MiR-4782-3p inhibited cell proliferation in NSCLC by targeting USP14, ZEB2 and XIAP. Copyright © 2014 S. Karger AG, Basel.


Su D.,PLA Logistical Engineering University | Su D.,Design Institute of Chengdu Military Region | Tu Y.-Q.,PLA Logistical Engineering University | Luo J.-Y.,Chongqing University | And 3 more authors.
Measurement Science and Technology | Year: 2015

To improve frequency estimation accuracy, a frequency estimation algorithm based on cross information fusion was proposed. The algorithm was suitable for signals of short duration and low signal-to-noise ratio (SNR), which are common in engineering. Firstly, several different signal groups were obtained by grouping multisegment signals according to the guidelines of combination. Secondly, rotation factors were obtained according to complementary information of multisegment signals in each signal group. Thirdly, the average cross information fusion spectrum was achieved by the arithmetic mean value of all the cross information fusion spectra, which were calculated according to rotation factors. Finally, the average cross information fusion spectrum was peak-picked to get the frequency estimator. Algorithm analysis and experimental results showed that compared with the existing algorithms, the proposed algorithm was superior in precision, robustness, and noise immunity. © 2015 IOP Publishing Ltd.

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