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Song B.,Shanghai University | Zhang C.,Design Institute of Chengdu Military Region | Li G.,Shanghai University | Jin G.,Shanghai University | Liu C.,Shanghai University
Cellular Physiology and Biochemistry | Year: 2015

Background: Pancreatic ductal adenocarcinoma (PDAC) is an almost universally lethal disease. Deregulation or dysfunction of miRNAs contribute to cancer development. The role of miR-940 in PDAC remains unclear. Methods: The level of miR-940 in PDAC tissues and cell lines was measured by qRT-PCR. MiR-940 was over-expressed by miRNAs mimics transfection and reduced by miRNAs antisense oligonucleotides (ASO) transfection. Cell proliferation was analyzed by MTT assay and cell apoptosis was evaluated by FACS analysis. Targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. Myeloid differentiation primary response gene (88) (MyD88) protein level was assayed by immunohistochemistry and Western blot analysis. Results: Low miR-940 level and high MyD88 protein level in PDAC tissues were both correlated with low survival rate. Up-regulation of miR-940 inhibited PDAC cell lines growth while down-regulation induced cell growth. The 3' UTR of MyD88 was targeted by miR-940. Conclusions: Low level of miR-940 and high level of MyD88 in PDAC promoted PDAC cells growth which might be related to the low survival rate of PDAC patients. MiR-940 exerted its effect by targeting MyD88. © 2015 S. Karger AG, Basel. Source


Jiang M.,PLA Logistical Engineering University | Guo X.,PLA Logistical Engineering University | Xiong X.,Design Institute of Chengdu Military Region
Shiyou Xuebao, Shiyou Jiagong/Acta Petrolei Sinica (Petroleum Processing Section) | Year: 2011

Aluminum complex grease is a kind of multifunction grease. It is suited to central lubrication system for which has good pump ability. It has been used extensively in metallurgy area. The effect of composition, condition and additives on the properties of aluminum complex grease has been discussed. The thickening ability of aluminum complex soap in paraffin oil is superior to that of aluminum complex soap in naphthene oil. Increasing viscosity of base oil can improve the properties of aluminum complex grease. The method of trioxyaluminum tri-isopropylate is superior to that of aluminum isopropylate, because the technology is stable and the property is good. By optimization of composition and condition, the high performance aluminum complex grease has been prepared. It possesses high dropping point, good colloidal stability, shear stability, oxidation stability, water resistance property, anti-corrosion and anti-wear property. Source


Zhu Y.,Shanghai University | Zhang C.,Design Institute of Chengdu Military Region | Gu C.,Shanghai airport authority | Li Q.,Shanghai University | Wu N.,Shanghai University
Cellular Physiology and Biochemistry | Year: 2016

Background/Aims: Non-small cell lung cancer (NSCLC) tissues overexpress USP14, which promotes tumor cell proliferation and is associated with shorter overall survival time. Methods: The expression of USP14 was assayed in many types of cancers. USP14 was up-and down-regulated using appropriate plasmid or lentiviral vector constructs and its effects on proliferation, cell colony number, and apoptosis rate were measured. A human NSCLC cell line was inoculated into nude mice and the survival rates were recorded. Results: We found USP14 amplification and overexpression in many different cancers. The overexpression of USP14 in USP14 low-expression cell lines promoted cell proliferation and migration, whereas USP14 downregulation suppressed tumor cell proliferation, decreased tumor cell colony number, increased apoptosis rate, and decreased cell migration and invasion. Conclusion: USP14 plays an oncogenic role in various types of cancer, and may thus represent a new cancer therapy target. © 2016 S. Karger AG, Basel. Source


Liu C.,Shanghai University | Zhang C.,Design Institute of Chengdu Military Region | Mitchel R.E.J.,Chalk River Laboratories | Cui J.,Shanghai University | And 4 more authors.
Cell Death and Disease | Year: 2013

Toll-like receptor-4 (TLR4) plays a critical role in innate and acquired immunity, but its role in radio-resistance is unknown. We used TLR4 knockout (KO,-/-) mice and gut commensal depletion methods, to test the influence of TLR4 and its' in vivo agonist on basal radio-resistance. We found that mice deficient in TLR4 were more susceptible to IR-induced mortality and morbidity. Mortality of TLR4-deficient mice after IR was associated with a severe and persistent bone marrow cell loss. Injection of lipopolysaccharide into normal mice, which is known to activate TLR4 in vivo, induced radio-resistance. Moreover, TLR4 in vivo ligands are required for basal radio-resistance. We found that exposure to radiation leads to significant endotoxemia that also confers endogenous protection from irradiation. The circulating endotoxins appear to originate from the gut, as sterilization of the gut with antibiotics lead to increased mortality from radiation. Further data indicated that Myd88, but not TRIF, may be the critical adaptor in TLR4-induced radio-resistance. Taken together, these data strongly suggest that TLR4 plays a critical role in basal radio-resistance. Our data suggest, it is important not to give antibiotics that may sterilize the gut before the whole body irradiation. Further, these data also suggest that management of gut flora through antibiotic or possibly probiotic therapy may alter the innate response to the total body irradiation. © 2013 Macmillan Publishers Limited. All rights reserved. Source


Lei W.,Peoples Hospital of Mianzhu | Liu Y.-E.,New Era Stroke Care and Research Institute | Zheng Y.,Third Peoples Hospital of Chengdu | Qu L.,Design Institute of Chengdu Military Region
Medical Science Monitor | Year: 2015

Background: Oral squamous cell carcinoma (OSCC) is the sixth most common human malignancy worldwide. To develop new therapeutics requires elucidation of the underlying mechanism of OSCC pathogenesis. The role of miR-429 in OSCC remains unknown.Material/Methods: The level of miR-429 and ZEB1 in OSCC tissues and cell lines was measured by qRT-PCR. MiR-429 was downregulated by miRNAs antisense oligonucleotides (ASO) transfection and up-regulated by miRNAs mimics. Cell proliferation was analyzed by MTT assay. Cell apoptosis was revealed by FACS analysis. Targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay.Results: MiR-429 was down-regulated in OSCC tissues, and miR-429 overexpression inhibited OSCC cell lines growth and vice versa. Further, we found that miR-429 could inhibit zinc finger E-boxbinding homeobox 1 (ZEB1) expression, and that miR-429 and ZEB1 expression in OSCC tissues were negatively correlated.Conclusions: Our data demonstrate the tumor suppressor role of miR-429 in OSCC, and may provide a potential therapeutic target that warrants further investigation. © Med Sci Monit. Source

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