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Lee C.-C.,National Chung Hsing University | Lee C.-C.,Derlin Biotech Corporation Ltd. | Chiou L.-Y.,Kaohsiung Medical University | Wang J.-Y.,Kaohsiung Medical University | And 7 more authors.
The Scientific World Journal | Year: 2013

Supercritical fluid carbon dioxide extraction technology was developed to gain the active components from a Taiwan native plant, Zingiber officinale (ginger). We studied the biological effects of ginger extracts via multiple assays and demonstrated the biofunctions in each platform. Investigations of ginger extracts indicated antioxidative properties in dose-dependant manners on radical scavenging activities, reducing powers and metal chelating powers. We found that ginger extracts processed moderate scavenging values, middle metal chelating levels, and slight ferric reducing powers. The antibacterial susceptibility of ginger extracts on Staphylococcus aureus, Streptococcus sobrinus, S. mutans, and Escherichia coli was determined with the broth microdilution method technique. The ginger extracts had operative antimicroorganism potentials against both Gram-positive and Gram-negative bacteria. We further discovered the strong inhibitions of ginger extracts on lethal carcinogenic melanoma through in vivo xenograft model. To sum up, the data confirmed the possible applications as medical cosmetology agents, pharmaceutical antibiotics, and food supplements. © 2013 Chih-Chen Lee et al. Source


Lee C.-C.,National Chung Hsing University | Lee C.-C.,Derlin Biotech Corporation Ltd. | Chen H.-C.,Da - Yeh University | Ju H.-Y.,Da - Yeh University | And 5 more authors.
Biotechnology Progress | Year: 2010

The ultrasound-accelerated enzymatic synthesis of octyl hydroxyphenylpropionate (OHPP) from p-hydroxyphenylpropionic acid (HPPA) and octanol was investigated in this study. A commercially available immobilized lipase from Candida antarctica, Novozym® 435, was used as the biocatalyst. A three-level-three-factor Box-Behnken design experiment and response surface methodology were used to evaluate the effects of temperature, reaction time, and enzyme activity on percent yield of OHPP. The results indicated that temperature and enzyme activity significantly affected percent yield, whereas reaction time did not. A model for the synthesis of OHPP was established. Based on a ridge max analysis, the optimum conditions for OHPP synthesis were predicted to use a reaction temperature of 58.8°C, a reaction time of 14.6 h, and an enzyme activity of 410.5 PLU with a yield of 98.5%. A reaction was performed under these optimal conditions, and a yield of 97.5% ± 0.1% was obtained. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010 © 2010 American Institute of Chemical Engineers (AIChE). Source


Chen H.-C.,Da - Yeh University | Ju H.-Y.,Da - Yeh University | Wu T.-T.,Da - Yeh University | Liu Y.-C.,Derlin Biotech Corporation Ltd. | And 4 more authors.
Journal of Biomedicine and Biotechnology | Year: 2011

An optimal continuous production of biodiesel by methanolysis of soybean oil in a packed-bed reactor was developed using immobilized lipase (Novozym 435) as a catalyst in a tert-butanol solvent system. Response surface methodology (RSM) and Box-Behnken design were employed to evaluate the effects of reaction temperature, flow rate, and substrate molar ratio on the molar conversion of biodiesel. The results showed that flow rate and temperature have significant effects on the percentage of molar conversion. On the basis of ridge max analysis, the optimum conditions were as follows: flow rate 0.1mL/min, temperature 52.1 °C, and substrate molar ratio 1:4. The predicted and experimental values of molar conversion were 83.31±2.07 and 82.81±.98 , respectively. Furthermore, the continuous process over 30 days showed no appreciable decrease in the molar conversion. The paper demonstrates the applicability of using immobilized lipase and a packed-bed reactor for continuous biodiesel synthesis. Copyright © 2011 Hsiao-Ching Chen et al. Source


Ju H.-Y.,Da - Yeh University | Huang K.-C.,Da - Yeh University | Chen J.-H.,National Chung Hsing University | Liu Y.-C.,National Chung Hsing University | And 5 more authors.
JAOCS, Journal of the American Oil Chemists' Society | Year: 2010

The essence oil of the Alpinia oxyphylla seed has been used as a vasodilatatory and analgesic agent in pharmacology. The extraction of the essence oil in supercritical carbon dioxide (SC-CO2) from Alpinia oxyphylla seeds was investigated. Small particles were obtained after breaking open, sieving, and drying from the Alpinia oxyphylla seeds. The small particles were placed in a 5-L extraction tank in a temperature-controlled system. The CO2 flow rate of the system was set at 1 L/min in this study. Response surface methodology with a three-factor and three-level Box-Behnken experimental design was used to evaluate the effects of the reaction parameters such as extraction time (1, 2, 3 h), temperature (45, 55, 65 °C), and pressure (20, 30, 40 MPa), on the extraction yield of the essence oil from Alpinia oxyphylla seeds. The results indicate that the extraction pressure was the most important parameter affecting the yield of the essence oil. A model for the estimation of the yield was developed. Based on the analysis of ridge max, the optimal extraction conditions were established as an extraction time of 2.8 h, a temperature of 67.5 °C, and a pressure of 28.5 MPa, with an expected yield of 2.78%. Extraction of Alpinia oxyphylla essence oil in SC-CO2 under these optimal conditions was conducted, and a yield of 2.77 ± 0.19% was obtained. © 2010 AOCS. Source


Lee C.C.,National Chung Hsing University | Lee C.C.,Derlin Biotech Corporation Ltd. | Chen H.C.,Da - Yeh University | Ju H.Y.,Da - Yeh University | And 5 more authors.
Journal of Industrial Microbiology and Biotechnology | Year: 2012

A solvent-free system to produce octyl hydroxyphenylpropionate (OHPP) from p-hydroxyphenylpropionic acid (HPPA) and octanol using immobilized lipase (Novozym 435) as a catalyst in an ultrasoundassisted packed-bed bioreactor was investigated. Responsesurface methodology (RSM) and a three-level-three-factor Box-Behnken design were employed to evaluate the effects of reaction temperature (x 1), flow rate (x 2) and ultrasonic power (x 3) on the percentage of molar production of OHPP. The results indicate that the reaction temperature and flow rate were the most important variables in optimizing the production of OHPP. Based on a ridge max analysis, the optimum conditions for OHPP synthesis were predicted to consist of a reaction temperature of 65°C, a flow rate of 0.05 ml/min and an ultrasonic power of 1.74 W/cm 2 with a yield of 99.25%. A reaction was performed under these optimal conditions, and a yield of 99.33 ± 0.1% was obtained. © Society for Industrial Microbiology and Biotechnology 2011. Source

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