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Shen Y.,Dental Branch Of Cangzhou Peoples Hospital | Ma H.-Y.,Dental Branch Of Cangzhou Peoples Hospital | Zhang Y.-S.,Dental Branch Of Cangzhou Peoples Hospital | Wang J.,Dental Branch Of Cangzhou Peoples Hospital | Shi B.-Z.,Dental Branch Of Cangzhou Peoples Hospital
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: Autogenous iliac bone graft for repair of alveolar cleft often cannot achieve a stable therapeutic effect, and the graft resorption rate varies with each individual that is influenced by many factors. OBJECTIVE: To observe the bone resorption after bone marrow mesenchymal stem cell sheet transplantation with autogenous iliac cancellous bone for alveolar cleft repair. METHODS: Bilateral alveolar cleft models were prepared in dogs. Using the self-control method, bone marrow mesenchymal stem cell sheet combined with autogenous iliac bone (experimental group) and autogenous iliac bone alone (control group) were respectively implanted into the bilateral alveolar clefts. Mandible CT and microCT were used to compare the bone resorption rate, bone volume fraction, trabecular thickness, trabecular separation, and bone mineral density. RESULTS AND CONCLUSION: The bone resorption rate was significantly lower in the experimental group than the control group at 3 and 6 postoperative months (P < 0.01); while, the bone volume fracture and bone mineral density of the experimental group were significantly higher than those in the control group at postoperative 6 months (P < 0.05), but the trabecular separation of the experimental group was significantly lower than that in the control group (P < 0.05). In addition, there was no difference in the trabecular thickness between the two groups (P > 0.05). These findings indicate that bone marrow mesenchymal stem cell sheet transplantation combined with autogenous iliac bone graft can reduce bone resorption rate and meanwhile, promote new bone formation. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


Shen Y.,Dental Branch Of Cangzhou Peoples Hospital | Ma H.-Y.,Dental Branch Of Cangzhou Peoples Hospital | Zhang Y.-S.,Dental Branch Of Cangzhou Peoples Hospital | Wang J.,Dental Branch Of Cangzhou Peoples Hospital | Shi B.-Z.,Dental Branch Of Cangzhou Peoples Hospital
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: Some cleft lip and palate patients at 9-10 years of age (the canine root development about 1/2-2/3) cannot achieve a good orthodontic outcome after bone grafting, and moreover, there is no unified therapeutic procedure for patients at 7-10 years of age before and after bone grafting. Cone-beam CT can reflect the fine structure accurately under the premise not to destroy normal tissue structure, and have access to the relevant measurement data by matched computer software. OBJECTIVE: To compare the therapeutic effect of orthodontic surgery combined with alveolar cleft bone grafting at different order for treatment of complete unilateral cleft lip and palate. METHODS: Sixty patients with complete unilateral cleft lip and palate admitted at Cangzhou People’s Hospital from 2012 to 2013 were enrolled and divided into three groups with 20 in each group: group A, autologous iliac graft was done followed by 3 months of wearing orthodontic retainer; Group B, autologous iliac graft was done immediately after orthodontic treatment and then, orthodontic retainer was used for 3 consecutive months; group C, orthodontic retainer was used for 3 consecutive months following orthodontic treatment, and then, autologous iliac graft was performed followed by another 3 consecutive months of wearing orthodontic retainer. RESULTS AND CONCLUSION: There were significant differences between groups A and C in terms of SNA, SNB, ANB, U1-NA and L1-NB (P < 0.01). Group B was significantly higher than the group A in SNB and L1/NB (P < 0.05). Compared with the group B, the SNB and U1/NA were reduced, while the SNA and L1/NB were increased significantly in the group C (P < 0.05). These findings indicate that for complete unilateral cleft lip and palate patients, the orthodontic treatment before and after bone grafting is better than simple pre- or post-grafting orthodontic treatment, which is conductive to the stability of corrected maxilla. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


Shen Y.,Dental Branch Of Cangzhou Peoples Hospital | Ma H.-Y.,Dental Branch Of Cangzhou Peoples Hospital | Zhang Y.-S.,Dental Branch Of Cangzhou Peoples Hospital | Wang J.,Dental Branch Of Cangzhou Peoples Hospital | Shi B.-Z.,Dental Branch Of Cangzhou Peoples Hospital
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: It has been demonstrated that the effect of collagen/hydroxyapatite/recombinant human bone morphogenetic protein-2 composite graft is good, but there is no comparative analysis of this composite graft and autologous bone graft. OBJECTIVE: To detect the healing effect of autologous bone graft versus collagen/hydroxyapatite/recombinant human bone morphogenetic protein-2 composite graft in a rat model of unilateral complete cleft palate. METHODS: Firstly, we established the artificial unilateral complete cleft palate models in 32 Sprague-Dawley rats, and then the established animal models were randomly divided into control group and experimental group. The autologous iliac bone was transplanted into the fissures of control group, and the experimental group received collagen/hydroxyapatite/recombinant human bone morphogenetic protein-2 composite graft. After that, the activities of serum alkaline phosphatase and tartrate-resistant acid phosphatase, bone mineral densities in the neonatal palate, expressions of osteocalcin, osteoprotegerin, core binding factor, and osteoclast differentiation factor were detected at 1, 2, 3, 4 weeks after treatment. RESULTS AND CONCLUSION: Over time, the alkaline phosphatase activity increased gradually, while tartrate-resistant acid phosphatase activity decreased. Compared with the control group, the alkaline phosphatase activity was always higher (P < 0.05, P < 0.01) but the tartrate-resistant acid phosphatase activity was lower in the experimental group (P < 0.05, P < 0.01); the bone mineral density increased in both groups, but it was always higher in the experimental group than the control group (P < 0.01). The expression levels of osteocalcin, osteoprotegerin, and core-binding factor gene gradually rose in both groups, but they were always higher in the experimental group than the control group; in contrast, the expression of osteoclast differentiation factor was decreased in both groups, and it was lower in the experimental group than the control group. These findings indicate that the collagen/hydroxyapatite/recombinant human bone morphogenetic protein-2 composite graft has more advantages compared with bone autograft. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.

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