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Gosen, Japan

Patent
Denka Seiken Co. | Date: 2013-07-22

A latex agglutination method by which the measurement range is extended and the sensitivity of the measurement in the low concentration range is increased, is disclosed. The method for measuring a test antigen by latex agglutination uses two types of large and small particles, having different average particle sizes. Each latex particle is sensitized with an antibody which undergoes antigen-antibody reaction with the test antigen. The purity of the antibody immobilized on the latex particles is within a specific range. The ratio of the amount of the antibody immobilized per one small latex particle to the amount of the antibody immobilized per one large latex particle; the average particle size of the large latex particles; the average particle size of the small latex particles; the concentration of the large sensitized latex particles in the antigen-antibody reaction system; and the concentration of the small sensitized latex particles in the reaction system are within a specific range. The large sensitized latex particles and the small sensitized latex particles are reacted with the test antigen in the state suspended in a buffer, and then the agglutination of the sensitized latex particles is optically measured.


The object of the present invention is to provide a method for estimating the glomerular renal function in a convenient and non-invasive manner. As a result of intensive studies to achieve the above object, the present inventors found that there is a high correlation between the urinary megalin excretion rate and the estimated glomerular filtration rate (eGFR) in renal disease patients, and the glomerular filtration rate (GFR) can be estimated with high probability in a non-invasive manner by measuring the megalin level in the urine. This has led to the completion of the present invention.


Patent
DENKA SEIKEN Co. | Date: 2015-06-24

The present invention relates to a method of detecting urinary podocytes with a light microscope through use of an insoluble carrier-bound antibody that recognizes a protein specifically expressed on a podocyte surface, and more specifically, to a method of detecting urinary podocytes, including the following steps of (1) to (3) : (1) preparing an insoluble carrier-bound antibody by binding an insoluble carrier to an antibody that recognizes a protein specifically expressed on a podocyte surface; (2) bringing a urine sample of a test subject into contact with the insoluble carrier-bound antibody; and (3) observing the urine sample brought into contact with the insoluble carrier-bound antibody with a light microscope to count podocytes.


Patent
Denka Seiken Co. | Date: 2013-10-03

Disclosed is a novel method for measuring haemagglutinin of an influenza virus, which can construct an assay system in a shorter period of time than a sandwich immunoassay method using two kinds of anti-haemagglutinin antibodies. The method for measuring haemagglutinin of an influenza virus is achieved by a sandwich immunoassay method comprising sandwiching the haemagglutinin between a lectin which binds to the haemagglutinin but does not bind to an antibody, and an anti-haemagglutinin antibody which undergoes antigen-antibody reaction with the haemagglutinin.


Patent
Denka Seiken Co. | Date: 2014-03-12

The present invention provides a test kit capable of rapid and highly sensitive detection. The test kit is provided with a first member, which contains a region in which is held a labeling substance with a label immobilized on a substance that specifically binds with a substance to be detected, and a second member, which has a detection zone where the labeling substance is captured through the substance to be detected, which is connected downstream of the first member in the developing direction, and which allows the labeling substance contained in a liquid sample that flows in from the first member, to develop into the detection zone. The first member has a dropping region located furthermost upstream and containing a portion onto which the liquid sample is dropped, a labeling substance holding region connected to the second member and having a containing portion that contains the labeling substance and a non-containing portion that is located upstream of the containing portion and that does not contain the labeling substance, and a backflow prevention region, which is connected between the dropping region and the non-containing portion of the labeling substance holding region, and in which absorbency is set higher than in the dropping region.

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